Elucidating Essential Components of Bone Marrow Metastasis

阐明骨髓转移的重要组成部分

• 批准号: 8522170
• 负责人: Jungwoo Lee
• 金额: $ 8.94万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-03 至 2014-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8522170
• 关键词: 3-Dimensional; Adipocytes; Adipose tissue; Biocompatible Materials; Blood Circulation; Blood Vessels; Bone Marrow; Bone Marrow Neoplasms; Bone Marrow Stem Cell; Breast; Cancer Biology; Cell Culture Techniques; Cells; Cessation of life; Development; Discontinuous Capillary; Distant; Engineering; Engraftment; Goals; Growth; Growth and Development function; Hematopoietic stem cells; Human; Image; Implant; In Vitro; Location; Lung; Malignant Neoplasms; Mammary Neoplasms; Marrow; Mediating; Mesenchymal Stem Cells; Metastatic Neoplasm to the Bone Marrow; Microfluidics; Modeling; Molecular; Mus; Neoplasm Metastasis; Osteoblasts; Pharmaceutical Preparations; Positioning Attribute; Preparation; Process; Prostate; Prostatic Neoplasms; Public Health; Recruitment Activity; Research; Research Personnel; Simulate; Site; Skin; Stromal Cells; System; Technology; Therapeutic; Tissue Engineering; Tissue Model; Training; Tumor Biology; Undifferentiated; Universities; analog; bone; cancer cell; carcinogenesis; chemokine; improved; in vivo; leukemia; malignant breast neoplasm; microchip; microsystems; migration; neoplastic cell; oncology; osteogenic; prevent; prostate cancer cell; scaffold; therapeutic target; tumor; tumor growth; tumor microenvironment

DESCRIPTION (provided by applicant): Metastasis to the bone marrow is observed in a number of fatal cancers. Tumor cells preferentially migrate to the bone cavity as a function of composition of the bone marrow. We have developed a tissue engineered analogue of the bone marrow and plan to use this construct to identify the key stromal cellular components of a bone marrow metastatic niche. We propose to: 1) Develop a microfluidic platform to capture migratory tumor cells in vitro, 2) Create tumor-stroma microenvironments on-chip, and 3) In vivo comparison and treatment study of BMSC scaffolds on tumor growth. This project will provide essential training to candidate in microfluidics, imaging, and cancer biology during the K99 training period and propel him to be a successful independent investigator in the field of biomaterials and oncology as an R00 investigator at a research university.

描述（由申请人提供）：在许多致命的癌症中观察到向骨髓的转移。肿瘤细胞优先迁移到骨腔，这是骨髓组成的函数。我们已经开发了骨髓的组织工程类似物，并计划使用该构造来识别骨髓转移性壁基的关键基质细胞成分。我们提出：1）开发一个微流体平台，以在体外捕获迁移肿瘤细胞，2）在片上创建肿瘤 - 细胞瘤微环境，以及3）BMSC支架对肿瘤生长的体内比较和治疗研究。该项目将在K99培训期间为微流体，成像和癌症生物学的候选人提供必不可少的培训，并推动他成为研究大学的R00研究者，成为生物材料和肿瘤学领域的成功独立研究者。