Morphology, Imaging, and Instrumentation (Core B)

形态学、成像和仪器（核心 B）

• 批准号: 8657066
• 负责人: THOMAS C TRUSK
• 金额: $ 29.5万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8657066
• 关键词: Adult; Award; Biocompatible Materials; Biomedical Engineering; Cardiovascular Diseases; Cardiovascular system; Cell Separation; Cells; Cellular biology; Centers of Research Excellence; Charge; Client; Collection; Colon Carcinoma; Computer Assisted; Computer software; Computers; Confocal Microscopy; Contracts; Core Facility; Data; Data Analyses; Development; Developmental Biology; Education and Outreach; Educational workshop; Electron Microscopy; Environment; Equipment; Experimental Designs; Faculty; Fees; Flow Cytometry; Fostering; Funding; Future; Goals; Grant; Growth; Histology; Human; Human Resources; Image; Image Analysis; Imagery; Imaging technology; Immunohistochemistry; In Situ Hybridization; Institution; Investments; Leadership; Letters; Licensing; Maintenance; Methods; Microscope; Microscopy; Minor; Morbidity - disease rate; Morphology; National Center for Research Resources; National Heart, Lung, and Blood Institute; Performance; Phase; Positioning Attribute; Price; Procedures; Program Research Project Grants; Recovery; Recruitment Activity; Regenerative Medicine; Research; Research Infrastructure; Research Personnel; Research Project Grants; Resources; Running; Scientist; Series; Services; Sorting - Cell Movement; Source; South Carolina; Spain; Staging; Stem cells; Stretching; Students; Subcellular Anatomy; Supervision; System; Techniques; Technology; Textbooks; Three-dimensional analysis; Training; Transmission Electron Microscopy; Ultrasonography; United States National Institutes of Health; Universities; Wages; Work; base; cardiogenesis; computing resources; cost; design; digital; digital imaging; experience; experimental analysis; instrument; instrumentation; investigator training; medical schools; meetings; mortality; outreach program; programs; reconstruction; research study; tissue processing; tissue regeneration; tool

The Morphology, Imaging and Instrumentation Core (B) provides the space, facilities, supervision, and training for investigators who use histological methods, and advanced imaging technology to answer research questions. This core builds on research investments from NIH/NCRR Shared Instrumentation Grants, NIH/NCRR COBRE Phase I and II programs, and an NSF Research Infrastructure Improvement Award. Core B provides skilled personnel capable of performing and training users in highly specialized techniques, such as confocal imaging, 3D reconstruction, immunohistochemistry, in situ hybridization, cell sorting, ultrasound, and image analysis; and offers advice with experimental design and analysis of data. Specific aims are: (a) to maintain the resources for safe and effective use by properly trained research personnel; (b) to assist research projects perform high quality and state-of-the-art image acquisition and data analyses; (c) to help in the design and performance of immunohistochemical and in situ hybridization analyses; (d) to provide a state-of-the-art cell sorting service, and (e) provide programs of outreach and education to investigators using methods and instrumentation available in the core. Faculty and student investigators have access to core facilities to process tissue and operate the histological tools, microscopes, and analysis computers, and are assisted by core staff concerning experimental design, data interpretation and technical information to best utilize facilities and instrumentation. The Core has a 15-year record of providing state-of-the-art service and training to investigators at MUSC and throughout South Carolina. Core staff participates in a weeklong training workshop on confocal microscopy (since 2004), acts as a Beckman Coulter Center of Excellence for development of flow cytometry, and has strong interactions with imaging cores in COBREs at Clemson (Biomaterials) and the University of South Carolina (Colon Cancer).

形态，成像和仪器核心（b）提供了空间，设施，监督和 使用组织学方法和高级成像技术回答的研究人员的培训 研究问题。该核心以NIH/NCRR共享工具的研究投资为基础 赠款，NIH/NCRR COBRE第一阶段和II计划，以及NSF研究基础设施的改进 奖。 Core B提供了能够在高度专业化的情况下执行和培训用户的熟练人员 技术，例如共聚焦成像，3D重建，免疫组织化学，原位杂交，细胞 分类，超声和图像分析；并通过实验设计和数据分析提供建议。 具体目的是：（a）维护经过适当培训的研究的资源，以实现安全有效的利用 人员; （b）协助研究项目执行高质量和最先进的图像获取以及 数据分析； （c）帮助免疫组织化学和原位杂交的设计和性能 分析； （d）提供最先进的细胞分类服务，（e）提供外展计划和 使用核心中可用的方法和仪器对研究人员进行教育。教师和学生 研究人员可以使用核心设施来处理组织并操作组织学工具，显微镜， 和分析计算机，并得到有关实验设计的核心人员的协助，数据解释 和技术信息以最好地利用设施和仪器。核心有15年的记录 向MUSC和整个南卡罗来纳州的调查人员提供最先进的服务和培训。核 工作人员参加了为期一周的共聚焦显微镜培训研讨会（自2004年以来）担任贝克曼 流式细胞仪开发发展的卓越中心，与成像有很强的相互作用 克莱姆森（Biomatirials）和南卡罗来纳大学（结肠癌）的核心核心。