Human Embryonic Stem Cell Core laboratory

人类胚胎干细胞核心实验室

• 批准号: 8598899
• 负责人: CAROL B WARE
• 金额: $ 46.1万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-12-01 至 2017-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8598899
• 关键词: Address; Adoption; Affect; Appearance; Area; Biological; Cardiac Myocytes; Cell Culture Techniques; Cell Line; Cells; Data; Development; Discipline; Educational process of instructing; Electrons; Embryo; Environment; Epiblast; Epigenetic Process; Fostering; Funding; Generations; Goals; Housing; Human; Hypoxia; Incubators; Individual; Knowledge; Laboratories; Lead; Life; Maintenance; Metabolic; Metabolic Pathway; Metabolism; Methods; Microscope; Microscopic; Mus; Noise; Phenotype; Pluripotent Stem Cells; Population; Property; Public Health; Reagent; Registries; Research Personnel; Resources; Services; Staging; Stem Cell Research; Stem cells; Testing; Tissues; United States National Institutes of Health; Veins; Vendor; Work; X Chromosome; base; cell type; cellular imaging; clinical application; design; embryonic stem cell; human embryonic stem cell; human embryonic stem cell line; human stem cells; induced pluripotent stem cell; interest; metabolomics; natural Blastocyst Implantation; novel; pluripotency; preimplantation; programs; self-renewal; stem cell biology; tissue culture; tool; vector

Core A provides expertise and resources that are essential for successful completion of each of the Projects within this Program. Core A is housed in the Ellison Stem Cell Core (ESCC), a purpose-designed and built facility providing access to tissue culture hoods, a number of Zeiss microscopes, live cell imaging capability, and hypoxia-ready incubators. Furthermore, Core A provides access to validated reagents for the culture and maintenance of human pluripotent stem cells, minimizing experimental variability that can occur between different vendors or batches. Core A also teaches and maintains a "best practices" environment that minimizes deviations that can lead to contamination and culture drift. However, perhaps of greatest importance are the advantages that result from the co-localization of POI investigators within the ESCC. By aggregating researchers from different disciplines, the ESCC aggregates expertise. This allows researchers from one laboratory to immediately benefit from advances made by other laboratories, facilitates the identification of important biological differences between pluripotent lines, and fosters collaborative efforts that benefit from contributions from multiple groups; a recent example being the adoption of vector-free methods for induced pluripotent stem cell (iPSC) generation. Core A will facilitate the goals of the overall POI through the provision of core services, by placing its knowledge and resources at the disposal of POI investigators, and by developing new knowledge and capabilities. During the current funding period Core A has made important contributions toward furthering our knowledge of stem cell biology. The current application proposes to continue in this vein by characterizing a new generation of "naive-like" human pluripotent stem cell lines, by developing culture conditions that will enhance the predictability with which human pluripotent stem cell lines generate tissues of interest, and by seeking to identify metabolic drivers of pluripotency and directed differentiation.

核心A提供了对成功完成此计划中每个项目至关重要的专业知识和资源。核心A位于Ellison干细胞核心（ESCC）中，这是一个专门设计和建造的设施，可访问组织培养引擎盖，许多Zeiss显微镜，实时细胞成像能力和缺氧就可以的孵化器。此外，核心A提供了对人类多能干细胞培养和维持经过验证的试剂的访问，从而最大程度地减少了不同供应商或批次之间可能发生的实验变异性。核心A还教授和维护“最佳实践”环境，该环境最大程度地减少了可能导致污染和文化漂移的偏差。但是，也许是最大的 重要性是ESCC中POI研究者共定位的优势。通过汇总来自不同学科的研究人员，ESCC汇总了专业知识。这使一个实验室的研究人员能够立即受益于其他实验室的进步，从而促进了多能线索之间重要的生物学差异的识别，并促进了从多个小组的贡献中受益的协作努力；最近的一个例子是采用无矢量方法来诱导多能干细胞（IPSC）产生。 核心A将通过提供核心服务，将其知识和资源置于POI调查人员以及开发新知识和能力来促进整体POI的目标。在当前的资金期间，核心A为进一步的干细胞生物学知识做出了重要的贡献。当前的应用建议通过发展新一代的“天真状”人类多能干细胞系，通过发展培养条件来继续进行这种静脉，从而增强人类多能干细胞线产生感兴趣的组织的可预测性，并寻求识别多能性和有指导分化的代谢驱动力。