Human Embryonic Stem Cell Core Laboratory

人类胚胎干细胞核心实验室

基本信息

批准号：
7903428
负责人：
CAROL B WARE
金额：
$ 58.86万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/7903428
关键词：
129 Mouse Aliquot Antiepileptic Agents Basic Science Binding Biology Butyrates Canis familiaris Carbon Cell Culture Techniques Cell physiology Chickens Collaborations Commit Communities Conditioned Culture Media Cryopreservation Dependency Dicer Enzyme Drosophila genus Embryo Employee Strikes Ethanol Fatty Acids Felis catus Fibroblasts Fred Hutchinson Cancer Research Center Functional RNA Funding Generations Genetic Translation Gifts Goals Human Institution Karyotype Ketone Bodies Laboratories Macaca mulatta Maintenance Marshal Messenger RNA Methods MicroRNAs Mink Mission Mus Phenotype Plants Play Prevalence Process RNA Research Design Research Personnel Research Support Resources Role Stem Cell Research Stem cells Testing Time Training Trichostatin A Undifferentiated United States National Institutes of Health Universities Valproic Acid Volatile Fatty Acids Washington Work Zebrafish base embryonic stem cell human embryonic stem cell human embryonic stem cell line improved investigator training matrigel mutant programs self-renewal stem cell differentiation

项目摘要

The hESC Core has been in existence since 2003, and has acquired and partially characterized 14 of the 21 NIH approved hESC lines. During this time it has established a reputation as an effective resource for hESC expertise within a highly collaborative scientific community. The Core will continue to assist PPG and UW/FHCRC investigators,characterize and store ESC lines, make the lines and derivatives available to investigators within the parameters of the MTA's, train investigators,devise new methods of hESC culture and characterize mico(mi)RNAsin undifferentiated and differentiated hESCs. In Specific Aim 1 we will support hESC research at UW/FHCRC by acquiring and characterizing the remaining seven NIH approved hESC lines, we will build an ES cell zoo,and we will continue to provide advice and assistance to UW/FHCRC investigators. In Specific Aim 2 we will develop second generation hESC culture conditions, building upon our initial observation that the 4 carbon fatty acid, butyrate,can support hESC self renewal in the absence of conditioned medium. We will also examine the effects of various matrices in the presence of butyrate. In Specific Aim 3 we will characterize all the NIH-Approved hESC lines for RNA and micro RNA Profiles, studies designed to reveal the function of microRNAs in hESCs.

自2003年以来，HESC核心一直存在，并获得了21个中的14个。 NIH批准了HESC线。在此期间，它已建立了hESC的有效资源的声誉高度协作的科学界的专业知识。核心将继续协助PPG和 UW/FHCRC调查人员，表征和存储ESC线路，使线路和衍生物可用 MTA参数中的调查人员，火车调查人员，设计了hESC文化的新方法并表征MICO（MI）RNASIN未分化和分化的hESC。在特定目标1中，我们将通过获取和表征UW/FHCRC的HESC研究剩余的七个NIH批准的hESC线，我们将建立一个ES Cell Zoo，我们将继续提供对UW/FHCRC调查人员的建议和帮助。在特定目标2中，我们将发展第二代 hESC培养条件，基于我们最初的观察，即4个碳脂肪酸，丁酸酯可以在没有条件培养基的情况下，支持hESC自我更新。我们还将研究在丁酸酯存在下各种矩阵。在特定目标3中，我们将表征所有NIH批准的 RNA和微RNA轮廓的hESC线，旨在揭示microRNA在中的功能的研究 hESC。