National Epidemiologic Survey on Alcohol and Related Conditions: DNA Repository

全国酒精及相关疾病流行病学调查：DNA 存储库

• 批准号: 8386959
• 负责人: JAY Arnold TISCHFIELD
• 金额: $ 229.23万
• 依托单位: RUTGERS, THE STATE UNIV OF N.J.
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-12-01 至 2015-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8386959
• 关键词: Accidents; Alcoholism; Alcohols; Aliquot; Automation; Bacterial DNA; Biocompatible Materials; Biological Assay; Biological Preservation; Caring; Cells; Chemistry; Collection; DNA; DNA Repository; DNA Sequence; Disease; Dyes; Engineering; Ensure; Environment; Epidemiology; Equipment and supply inventories; Ethnic Origin; Event; Fingerprint; Future; Gender; Genome; Genomics; Human; Laboratories; Libraries; Liquid substance; Management Information Systems; Measurement; Medical; Molecular Genetics; Molecular Weight; Monitor; National Institute on Alcohol Abuse and Alcoholism; Nucleic Acids; Population; Prevention strategy; Process; Productivity; Qualifying; Quality Control; RNA; Robot; Saliva; Sampling; Secure; Sequence Analysis; Services; Surveys; System; Technology; Tube; Universities; Violence; alcohol use disorder; base; cost; cost effective; design; gene environment interaction; genetic epidemiology; genetic risk factor; instrument; next generation; next generation sequencing; novel; programs; response; social; success; treatment strategy

DESCRIPTION (provided by applicant): The National Epidemiological Surveys on Alcohol and Related Conditions (NESARC I and II) are the largest and most ambitious epidemiological surveys on alcoholism and related disorders in the civilian, non-institutionalized US population. NESARC-III is even more ambitious as it aims to collect saliva samples from as many as 46,500 subjects and extract DNA from each in an effort to identify genetic risk factors for alcohol use disorders (AUDs) and co-morbid disorders through large-scale DNA re-sequencing and to examine gene-environment interactions. We propose to establish the Saliva DNA Repository for the NIAAA NESARC-III study. Our Aims are: 1. Genomic DNA Extraction from Human Saliva Samples: We will employ state-of-the-art automation and bead-based chemistry to enrich for high molecular weight human genomic DNA relatively free of contaminants and of RNA and undesirable DNA species (i.e., bacterial DNA contamination). 2. Comprehensive Nucleic Acid Quality Control: We have engineered and validated a novel, comprehensive analytical and functional quality control (QC) program that ensures the highest quality of DNA and its utility for future sequencing analyses. Analytical QC consists of absorbance and dye-based concentration and purity measurements, precision non-contact volume measurements, and specific quantification of the amount of human genomic DNA in each sample. Functional QC employs a high-throughput, molecular genetic fingerprinting SNP assay (the RUID). We will also use several different Next Generation sequencing platforms to demonstrate the utility of this saliva DNA for future sequencing. 3. Integrated Nucleic Acid Storage and Distribution: All NESARC-III samples will be stored at -80 degrees C in dedicated freezers that are monitored 24/7/365 in a secure facility that contains state-of-the-art redundant power and HVAC systems. All sample extraction, QC, storage, biomaterial requests and distribution workflows are documented and managed by the RUCDR STARLIMS, an enterprise-level laboratory information management system.

描述（由申请人提供）：全国酒精及相关病症流行病学调查（NESARC I 和 II）是针对美国非机构化平民人口中酒精中毒及相关疾病开展的规模最大且最雄心勃勃的流行病学调查。 NESARC-III 的目标更加雄心勃勃，它的目标是收集多达 46,500 名受试者的唾液样本，并从每个受试者中提取 DNA，以便通过大规模 DNA 识别酒精使用障碍 (AUD) 和共病疾病的遗传风险因素重新测序并检查基因与环境的相互作用。 我们建议为 NIAAA NESARC-III 研究建立唾液 DNA 存储库。 我们的目标是： 1. 从人类唾液样本中提取基因组 DNA：我们将采用最先进的自动化和基于微珠的化学方法来富集高分子量人类基因组 DNA，相对不含污染物、RNA 和不需要的 DNA 种类（即细菌 DNA 污染）。 2. 全面的核酸质量控制：我们设计并验证了一种新颖、全面的分析和功能质量控制 (QC) 程序，可确保 DNA 的最高质量及其对未来测序分析的实用性。分析 QC 包括吸光度和基于染料的浓度和纯度测量、精密非接触式体积测量以及每个样品中人类基因组 DNA 量的具体定量。功能 QC 采用高通量分子遗传指纹 SNP 测定 (RUID)。我们还将使用几种不同的下一代测序平台来展示这种唾液 DNA 在未来测序中的实用性。 3. 集成核酸储存和分配：所有 NESARC-III 样本都将储存在 -80 摄氏度的专用冰柜中，并在配备最先进冗余电源和 HVAC 的安全设施中进行 24/7/365 全天候监控系统。所有样品提取、质量控制、存储、生物材料请求和分配工作流程均由企业级实验室信息管理系统 RUCDR STARLIMS 记录和管理。