Central Biochemistry Laboratory of the CKiD Consortium

CKiD 联盟中央生物化学实验室

基本信息

批准号：
8581911
负责人：
GEORGE J SCHWARTZ
金额：
$ 35万
依托单位：
UNIVERSITY OF ROCHESTER
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-09-01 至 2018-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8581911
关键词：
Address Albumins Ancillary Study Archives Award Bicarbonates Biochemical Biochemistry Biological Assay Biological Markers Blood Blood specimen Calcium Cardiovascular Diseases Cardiovascular system Child Chronic Kidney Failure Clinical Collaborations Collection Creatinine Data Data Coordinating Center Databases Disease Progression Dry Ice Electrolytes Electronic Mail Epidemiology Equation Fingers Freezing Funding Generations Glomerular Filtration Rate Glucose Goals Growth Guidelines Hair Health Health Status Human Hypertension Hyperuricemia Inflammation Instruction Iohexol Kidney Kidney Diseases Kidney Function Tests Label Laboratories Laboratory Personnel Lipids Manuals Manuscripts Measurement Measures Metabolic Methodology Microalbuminuria Monitor Nail plate National Institute of Diabetes and Digestive and Kidney Diseases Nephrons Neurocognition Observational Study Online Systems Persons Phosphorus Pilot Projects Plasma Population Procedures Process Prospective Studies Proteins Protocols documentation Publications Quality Control Reagent Renal function Reproducibility Research Personnel Risk Risk Factors Role Sampling Scientist Serum Shipping Ships Site Specimen Spottings Staging Standardization Telephone Testing Time Training Transportation Tube United States National Institutes of Health Uric Acid Urine Vial device Visit Work abstracting base clinical research site cohort data integrity improved instrument instrumentation meetings post gamma-globulins prostaglandin R2 D-isomerase public health relevance repository sample collection skeletal success symposium tool urinary

项目摘要

DESCRIPTION (provided by applicant): The Central Biochemistry Laboratory (CBL) serves the subjects and investigators of the continuing Prospective Study of Chronic Kidney Disease in Children (CKiD). The CBL provides participating clinical sites with protocols, kits, reagents, supplies, and transportation required for performing iohexol-based glomerular filtration rate (GFR) studies and collecting blood and urine for analysis at URMC and other laboratories. Blood analytes include electrolytes, BUN, creatinine, glucose, calcium, phosphorus, albumin, uric acid, lipid screen, hsCRP, intact PTH, and cystatin C. Urine analytes include creatinine, protein, and microalbumin. The CBL has determined optimal conditions for transporting these analytes, and provides ambient shippers for each visit and quarterly dry ice shippers for frozen specimens. The CBL also provides the kits and instructions for collection and shipment of blood, plasma, sera, urine, hair, and nail samples to NIH repositories. Biochemical data from each CKiD visit are reviewed and entered into Nephron, the CKiD data base. The CBL provides training at annual CKID meetings and is readily available to provide information, instruction, and support during CKiD visits, as needed by the site coordinators. The CBL contributes expertise in performing biochemical assays and clinical interpretation of the results. The CBL collaborates regularly with the Data Coordinating Center (DCC) to optimize GFR studies and to construct GFR estimating equations used during odd number visits at which no iohexol study is performed, maintain the Manual of Procedures, assure the quality control of all assays, and provide continuity of biochemical assays through changes in instrumentation. The CBL participates in all Steering Committee meetings and conference calls and collaborates with investigators in the generation of abstracts, presentations, and manuscripts, which further document the success of the CKiD study. The aims of the CBL center on continuing to provide accurate, precise, and efficient measures of iohexol-based GFR and biochemical measurements of general kidney health. A pilot study to determine if fingerprick samples can replace serum collections for the iohexol GFR continues during this cycle. We plan to develop better GFR estimating equations, particularly for the children of Cohort 2, who have higher levels of GFR than those of Cohort 1. The CBL has submitted concept sheets to further determine if hyperuricemia predicts renal progression or hypertension, if microalbuminuria is a better predictor of renal progression than urinary protein, and to examine beta trace protein to determine if it is useful in predicting GFR. Finally, the CBL will continue to provide to the participating sites subject- and visit specific- laboratory kits for the collection, handling, and transport of samples, as well as receive, process, and analyze these samples on a daily basis, and provide timely data entry into Nephron. The long term goal is to better characterize the CKiD population biochemically, maintain the integrity of the data, and to optimize the collection of specimens in a manner that maintains recruitment and retention of CKiD subjects.

描述（由申请人提供）：中央生物化学实验室（CBL）为儿童慢性肾脏病的前瞻性研究的受试者和研究者（CKID）服务。 CBL为进行基于iohexol的肾小球过滤率（GFR）研究所需的方案，试剂盒，试剂，供应和运输提供了参与的临床部位，并在URMC和其他实验室收集血液和尿液进行分析。血液分析物包括电解质，BUN，肌酐，葡萄糖，钙，磷，白蛋白，尿酸，脂质筛选，HSCRP，完整的PTH和CystatinC。 CBL已确定运输这些分析物的最佳条件，并为每次访问和季度干冰托运人提供环境托运人，用于冷冻样品。 CBL还为NIH存储库提供了血液，血浆，血清，尿液，头发和指甲样品的套件和说明。每次CKID访问中的生化数据都会审查并输入到CKID数据库Nephron。 CBL在年度CKID会议上提供培训，并且可以根据网站协调员需要在CKID访问期间提供信息，指导和支持。 CBL在进行生化测定和结果的临床解释方面有助于专业知识。 CBL定期与数据协调中心（DCC）合作，以优化GFR研究并构建在奇数访问期间使用的GFR估计方程，在奇数访问中使用iohexol研究，维护程序手册，确保所有测定法的质量控制，并提供通过仪器进行变化的生物化学分析的连续性。 CBL参加了所有指导委员会会议和电话会议，并与调查人员合作，在摘要，演示和手稿的产生中，进一步记录了CKID研究的成功。 CBL中心继续提供基于iohexol的GFR和一般肾脏健康的生化测量的准确，精确和有效的衡量标准的目的。一项试点研究以确定指纹样品是否可以在此周期中继续替代Iohexol GFR的血清收集。 We plan to develop better GFR estimating equations, particularly for the children of Cohort 2, who have higher levels of GFR than those of Cohort 1. The CBL has submitted concept sheets to further determine if hyperuricemia predicts renal progression or hypertension, if microalbuminuria is a better predictor of renal progression than urinary protein, and to examine beta trace protein to determine if it is useful in predicting GFR.最后，CBL将继续向参与的站点提供主题，并访问特定的实验室套件，以进行样品的收集，处理和运输以及每天接收，处理和分析这些样品，并及时地进入Nephron。长期目标是更好地以生物化学的形式表征CKID人群，保持数据的完整性，并以维持CKID受试者的募集和保留方式优化标本的收集。