Neural Processing in Behaving Adolescent Rats

青春期大鼠行为的神经处理

• 批准号: 8454981
• 负责人: Nicholas W Simon
• 金额: $ 5.39万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-01 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8454981
• 关键词: Address; Adolescence; Adolescent; Adult; Affect; Age; Animal Model; Anterior; Attention; Basic Science; Behavior; Behavioral; Behavioral inhibition; Biological Neural Networks; Brain; Brain region; Climacteric; Cognitive; Communication; Complex; Corpus striatum structure; Cues; Data; Data Analyses; Development; Dissociation; Dorsal; Drug Addiction; Drug abuse; Electrophysiology (science); Ensure; Event; Goals; Intervention; Investigation; Measures; Mediating; Mental disorders; Methodology; Nature; Neurobiology; Neurons; Noise; Outcome; Pattern; Performance; Phase; Prefrontal Cortex; Procedures; Process; Public Health; Rattus; Rodent; Staging; Structure; Testing; Time; Training; addiction; age group; age related; base; cingulate cortex; design; expectation; experience; information processing; insight; motivated behavior; novel; public health relevance; relating to nervous system; research study; response; social; stressor

DESCRIPTION (provided by applicant): This application outlines a training plan and experimental procedures to ensure that the applicant will receive technical and intellectual development toward independence. The application focuses on neuronal bases of motivated behavior in adolescents, a topic with important implications for basic science and public health. Adolescents are particularly vulnerable to drug addiction and other psychopathological illnesses. This vulnerability may be a result of ongoing prefrontal cortex (PFC) and striatal development during adolescence. However, little is known about how neuronal activity within these brain regions during the expression of motivated behavior differs as a function of age. The applicant has developed the Cued Response Inhibition Task, or CRIT, to measure the ability to withhold a response during an inhibitory cue, then respond promptly following cue termination. Preliminary data demonstrate that adolescent rats are unable to respond as quickly as adults following cue termination, a behavioral difference that appears to be related to inability to adequately sustain attention to the cue. It is hypothesized that this behavioral profile will be associated with aberrant neuronal encoding of task events in PFC and striatal subregions in adolescent compared to adult rats. To test this hypothesis, two aims are proposed, both of which involve application of methodologies and data analysis that are novel for the applicant. The first aim will involve recording single unit activity simultaneously from two brain regions during CRIT performance: anterior cingulate cortex (ACC) and dorsal striatum (DS), These regions were chosen because previous rodent recording studies demonstrate that ACC phasic responses correlate with sustained attention, and that neuronal encoding in the DS differs in adolescents from adults during motivated behavior. Neural activity will be analyzed during cue presentation, instrumental response, and outcome expectation/delivery, then compared between adults and adolescents. In the second aim, neuronal interactions within ACC and DS, as well as communication between ACC and DS during CRIT will be quantified. This will involve within, and between region analysis of noise correlations between unit pairs, local field potential (LFP) spectral power, and phase-locking between single unit activity and LFP. This will provide information about communication and direction of information flow between ACC and DS, and how this relates to behavior in adolescent and adult rats. Collectively, these experiments will provide novel information on how neural processing differs between adult and adolescent rats, and how these differences correlate with age related behavioral differences.

描述（由申请人提供）：本申请概述了培训计划和实验程序，以确保申请人获得技术和智力发展以实现独立性。该申请的重点是青少年动机行为的神经元基础，这是对基础科学和公共卫生的重要影响的主题。青少年特别容易受到药物成瘾和其他心理病理学疾病的影响。这种脆弱性可能是青春期前额叶皮层（PFC）和纹状体发育的结果。然而，对于这些动机行为表达期间这些大脑区域内的神经元活动的差异与年龄的函数不同，知之甚少。申请人已经制定了提示响应抑制任务或犯罪，以衡量在抑制提示期间扣留响应的能力，然后在提示终止后立即做出响应。初步数据表明，青少年大鼠在提示终止后无法像成年人一样快地做出反应，这一行为差异似乎与无法充分保持对提示的关注有关。假设与成年大鼠相比，这种行为特征将与青少年的PFC和纹状体子区域中任务事件的异常神经元编码有关。为了检验这一假设，提出了两个目标，这两者都涉及对申请人新颖的方法和数据分析的应用。第一个目标 在攻击性能期间，参与来自两个大脑区域的单位活性：前扣带回皮层（ACC）和背纹状体（DS），之所以选择这些区域，是因为先前的啮齿动物记录研究表明，ACC Phasic响应与持续的注意力相关，与成人的神经元编码相关。在提示表现，工具反应和结果期望/传递过程中，将分析神经活动，然后比较成人和青少年之间。在第二个目标中，将量化ACC和DS内的神经元相互作用，以及Crit期间ACC和DS之间的通信。这将涉及内部和区域分析单位对之间的噪声相关性，局部场电位（LFP）光谱功率以及单个单位活动和LFP之间的相锁定。这将提供有关ACC和DS之间信息流的通信和方向的信息，以及与青少年和成年大鼠的行为之间的关系。总的来说，这些实验将提供有关成年大鼠和青少年大鼠之间神经过程如何不同的新信息，以及这些差异与年龄相关的行为差异如何相关。