Transgenic Mouse

转基因小鼠

基本信息

批准号：
8300294
负责人：
MAX S. WICHA
金额：
$ 18.37万
依托单位：
UNIVERSITY OF MICHIGAN AT ANN ARBOR
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-06-01 至 2017-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8300294
关键词：
129 Mouse Alleles Animal Model Animals Assisted Reproductive Technology Bacteria Bacterial Artificial Chromosomes Biological Breeding Cancer Center Cancer Center Support Grant Cancer Etiology Cell Culture Techniques Cells Chimera organism Chromosomes Colon Carcinoma Consultations Cryopreservation DNA Data Data Analyses Derivation procedure Drug resistance ES Cell Line Electroporation Embryo Embryo Transfer Engineering Equipment Experimental Designs Fertilization in Vitro Fostering Freezing Gel Gene Targeting Gene-Modified Generations Genes Genetic Genetic Engineering Genetically Engineered Mouse Genotype Human Human Resources Inbred F344 Rats Infectious Agent Institution Investigation Knockout Mice Laboratories Life Literature Malignant Neoplasms Malignant neoplasm of pancreas Malignant neoplasm of prostate Medicine Microinjections Micromanipulation Modeling Modification Molecular Biology Monitor Mothers Mouse Strains Mouse, Founder, Transgenic Mus Mutation Mycoplasma Organism Partner in relationship Phenotype Physiologic pulse Plasmids Procedures Production Publishing Rattus Reagent Recovery Reporter Genes Reproductive Techniques Research Research Personnel Resistance Resources Restriction Mapping Review Literature Scientist Services Skin Cancer Source Specific Pathogen Frees Stem Cell Research Technology Testing Training Transgenes Transgenic Mice Transgenic Organisms University of Michigan Comprehensive Cancer Center Vial device Virus Work Zinc Fingers antibiotic G 418 assisted reproduction base blastocyst design egg embryonic stem cell fetal bovine serum gene function germ free condition homologous recombination in vivo innovation interest malignant breast neoplasm malignant stomach neoplasm meetings member new technology nuclease pathogen pup quality assurance recombinase repository sperm cell stem tool transmission process vector web site zygote

项目摘要

The Transgenic Core works with Investigators to generate animal models what will increase our understanding of cancer and the function of genes in involved in cancer. This Core was established in 1989 and produces transgenic mice and rats, and gene-targeted mice (knockouts) for Cancer Center members. The Transgenic Core maintains specialized equipment for micromanipulation, mouse embryonic stem (ES) cell culture, embryo cryopreservation, bacterial artificial chromosome (BAC) recombineering. This collaborative Core combines the expertise of Center members in the molecular biology of Important genes with the Core's expertise in producing genetically engineered mice, Unique capabilities that set this Transgenic Core apart are 1) guaranteed production of transgenic mice and rats. 2) routine production of BAC transgenic mice, 3) production of transgenic mice In unique genetic backgrounds, 4) gene targeting In C57BL/6 ES cell lines in addition to 129/Sv ES cells, 5) de novo derivation of mouse ES cell lines, 6) genetic modification of BACs, 7) open access to reagents and equipment, and 8) training in ES cell culture and micromanipulation. Access to the Transgenic Core eliminates the need for Investigators to purchase specialized equipment and train personnel in embryo micromanipulation, ES cell culture, and BAG recomblneering. Consultation on all aspects of transgenic and ES cell research is provided, from the design of transgenes and conditional targeting vectors to mouse breeding and phenotype analysis. We deliver an average of nine transgenic founder mice and guarantee that at least three founders will be produced for each DNA construct submitted to the Core, The Core electroporates totipotent ES cells with targeting vectors, selects 480 ES cell clones, and provides Investigators with ES cell DNA to screen for homologous recombination with targeting vectors. We guarantee that ES cell clones with desired genetic changes will be microinjected Into at least 60 mouse blastocysts to produce ES cell-mouse chimeras. The efficiency of these procedures meets or exceeds published values in the literature. A full suite of assisted reproductive technologies is offered; mouse cryopreservation, mouse In vitro fertilization, sperm cryopreservation, recovery of mice from cryopreserved embryos or sperm. Center members have taken advantage of these capabilities to establish develop models of gastric cancer, pancreatic cancer, skin cancer, colon cancer, prostate cancer, breast cancer, and medulioblastoma.

转基因核心与研究人员合作生成动物模型，这将增进我们对癌症和癌症相关基因功能的了解。该核心成立于 1989 年，为癌症中心成员生产转基因小鼠和大鼠以及基因靶向小鼠（基因敲除）。转基因核心拥有用于显微操作、小鼠胚胎干 (ES) 细胞培养、胚胎冷冻保存、细菌人工染色体 (BAC) 重组的专用设备。这个协作核心结合了中心成员在重要基因分子生物学方面的专业知识和核心在生产基因工程小鼠方面的专业知识，使该转基因核心与众不同的独特功能是 1) 保证转基因小鼠和大鼠的生产。 2) BAC 转基因小鼠的常规生产，3) 在独特的遗传背景下生产转基因小鼠，4) 除 129/Sv ES 细胞外，在 C57BL/6 ES 细胞系中进行基因靶向，5) 小鼠 ES 细胞系的从头衍生，6）BAC 的基因改造，7）开放试剂和设备，8）ES 细胞培养和显微操作培训。获得转基因核心研究人员无需购买胚胎显微操作、ES 细胞培养和 BAG 重组方面的专门设备和培训人员。提供转基因和胚胎干细胞研究各个方面的咨询，从转基因和条件靶向载体的设计到小鼠育种和表型分析。我们平均提供 9 只转基因创始小鼠，并保证提交给 Core 的每个 DNA 构建体将产生至少 3 个创始小鼠，Core 使用靶向载体电穿孔全能 ES 细胞，选择 480 个 ES 细胞克隆，并为研究人员提供 ES 细胞用于筛选与靶向载体同源重组的 DNA。我们保证将具有所需遗传改变的 ES 细胞克隆显微注射到至少 60 个小鼠囊胚中，以产生 ES 细胞-小鼠嵌合体。这些程序的效率达到或超过文献中公布的值。提供全套辅助生殖技术；小鼠冷冻保存、小鼠体外受精、精子冷冻保存、从冷冻胚胎或精子中回收小鼠。中心成员利用这些能力建立了胃癌、胰腺癌、皮肤癌、结肠癌、前列腺癌、乳腺癌和髓母细胞瘤的模型。