MAPP Research Networdk TIssue Analysis & Technology Core

MAPP 研究网络k 组织分析

• 批准号: 8335097
• 负责人: M. Scott Lucia
• 金额: $ 75万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-09 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8335097
• 关键词: Area; Biological Assay; Body Fluids; Collection; Computer Assisted; Core Facility; Diagnostic; Drug Kinetics; Enzyme-Linked Immunosorbent Assay; Gel Chromatography; Generations; Genomics; Genotype; Histopathology; Image; Image Analysis; Immunohistochemistry; Mass Spectrum Analysis; Microtomy; Oxidative Stress; Paraffin; Pathogenesis; Patients; Pelvic Pain; Performance; Process; Protein Array; Proteins; Proteome; Proteomics; RNA; Research; Resources; Reverse Transcriptase Polymerase Chain Reaction; Site; Specimen; Specimen Handling; Syndrome; Techniques; Technology; Tissue Microarray; Tissues; Woman; base; biobank; biological research; chronic pelvic pain; cytokine; insight; laser capture microdissection; men; metabolomics; morphometry; programs; response; success; two-dimensional

DESCRIPTION (provided by applicant): The overall aim of this application is to establish a Tissue Analysis and Technology Core (TATC) in support of the Multi-disciplinary Approach to the Study of Chronic Pelvic Pain (MAPP) Research Network as described in RFA-DK-07-003. The MAPP Research Network will utilize a multi-site and multi-disciplinary approach to provide new insights into the pathogenesis and treatment of Chronic Pelvic Pain Syndromes, and establish a new and valuable biorepository resource for current and future research efforts. In response to the RFA, the TATC will interact with all research entities in the MAPP Network and provide expertise and resources in the areas of: 1) Biorepository Collection, Management and Distribution for all body fluid and tissue specimens generated by the MAPP Discovery Sites, and the generation of specialized assay platforms from these specimens such as protein and/or tissue arrays, DMA or RNA extractions as needed for Discovery Site efforts or the Ancillary Projects Program; 2) Histopathology and Tissue Morphometry including diagnostic interpretation, paraffin processing and microtomy, cryomicrotomy, laser capture microdissection, immunohistochemistry, and computer-assisted imaging/ image analysis; 3) Proteomics/ Metabolomics by providing a state-of-the-art centralized mass spectrometry facility to assist with proper collection and handling of specimens, consultative assistance for proteomic and/or metabolomics studies, and performance of a wide variety of assays, including two-dimensional gel- and chromatography-based proteome profiling, protein arrays, cytokine arrays, multiplexed ELISA, RT-PCR, infrared imaging, mass spectrometry and NMR-based targeted and non-targeted metabolite profiling, pharmacokinetic studies, oxidative stress assessment, and mass spectrometry analyses; and 4) Genomics/ Genotyping by providing consultative assistance and a state-of-the-art genomics facility to assist with advanced genotyping techniques. It is our underlying hypothesis that the quality, reliability and applicability of biological research on pelvic pain syndromes can only be guaranteed through the use of high quality non-biased patient specimens that have been handled, processed, analyzed and interpreted in a uniform manner. The MAPP Research Network will be the largest research initiative of its kind for chronic pelvic pain syndrome, a common but understudied condition in men and women. The establishment of a Tissue Analysis and Technology Core Facility is crucial to the success of this research program.

描述（由申请人提供）：本申请的总体目的是建立组织分析和技术核心（TATC），以支持RFA-DK-07-003中所述的慢性骨盆疼痛研究（MAPP）研究网络的多学科方法。 MAPP研究网络将利用一种多站点和多学科的方法来提供有关慢性骨盆疼痛综合症的发病机理和治疗的新见解，并为当前和未来的研究工作建立了新的且有价值的生物措施资源。为了响应RFA，TATC将与MAPP网络中的所有研究实体进行互动，并在以下方面提供专业知识和资源：1）MAPP Discovery站点产生的所有身体流动和组织的生物膜收集，管理和分配，以及从这些蛋白质和/或组织或组织范围的范围或诸如诸如蛋白质或组织的discore discore或rna are necor necor necor necors或rna orna arrays或rna rane arrays，dmma dmma disma dismime disce rna或rna产生的标本中产生的，rne; 2）组织病理学和组织形态计量学，包括诊断解释，石蜡加工和显微镜切开术，冷冻切开术，激光捕获显微解剖，免疫组织化学以及计算机辅助成像/图像分析； 3）通过提供最先进的集中式质谱设施来帮助对蛋白质组学和/或代谢组学的咨询辅助以及进行多种测定法，包括基于二维的凝胶蛋白质组的蛋白质蛋白质群，cyeremine cytrive reprive reprive Rrive，成像，质谱和基于NMR的靶向和非靶向代谢产物分析，药代动力学研究，氧化应激评估和质谱分析； 4）基因组学/基因分型通过提供协商辅助和最先进的基因组学设施来帮助采用先进的基因分型技术。我们的基本假设是，只有通过使用已经以统一的方式处理，处理，分析和解释的高质量的非偏见的患者标本，才能保证生物学研究对骨盆疼痛综合征的质量，可靠性和适用性。 MAPP研究网络将是慢性骨盆疼痛综合症的最大研究计划，这是男性和女性常见但研究的一种研究。组织分析和技术核心设施的建立对于该研究计划的成功至关重要。