Molecular Mechanisms Core

分子机制核心

基本信息

批准号：
8565076
负责人：
Leslie Kozak
金额：
$ 26.56万
依托单位：
LSU PENNINGTON BIOMEDICAL RESEARCH CTR
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8565076
关键词：
Binding Cell Culture Techniques Cell Respiration Cell Separation Cells Cellular biology Charge Collaborations Communities Computer software Consult Consultations Custom DNA DNA Fingerprinting DNA Sequence Data Data Analyses Data Quality Development Ensure Equipment Experimental Designs Faculty Funding Gene Expression Profiling Genomics Genus Hippocampus Grant Histology Human Resources Image Individual Instruction Knowledge Label Laboratories Leadership Learning Libraries Link Louisiana Measures Methods Microarray Analysis Molecular Monitor Names Nutritional Nutritional Study Pathway interactions Postdoctoral Fellow Preparation Printing Process Production Protocols documentation Quality Control RNA Research Research Design Research Personnel Resources Robotics Running Scanning Scientist Sequence Alignment Services Signal Transduction Solutions Source Specimen Speed Students Sulfites Techniques Technology Time Training Western Blotting Writing aerobic glycolysis base bioimaging biological research cost cost effective design extracellular instrument instrumentation meetings next generation sequencing programs research study skills tissue culture web site

项目摘要

Overview of the Molecular Mechanisms Core: During the past five years of the NORC grant period major changes in the Molecular Mechanisms Core have occurred. These changes represent improvements occasioned by the introduction of major technologies in genomic analysis and in bioimaging, cell sorting and traditional histology. To accommodate these changes and to provide more services to more scientists, we have simplified the organization of the leadership to provide more funds for technical personnel who will establish the technologies in each subcore. We monitored Core service usage during this period and based on low levels of use, we eliminated the cell culture service. This allowed us to reallocate resources and to expand the Cell Signaling Service, now named the Cell Biology and Bioimaging Subcore. These changes are described in more detail below. Objectives of the Genomics Subcore: To perform DNA sequencing and fragment analysis service including bi-sulfite sequencing and standard sequencing of a broad range of DNA types To offer quantitative PCR instruments for cost effective shared use with support for experimental design, instrument and software use, and data analysis To offer full microarray analysis service including experiment consultation, RNA quality control, labeling of RNA, hybridization of microarray chips, scanning, and data production To provide next-generation sequencing including consultation for experimental design, grant writing support, library creation service or assistance, instrument run, and data analysis To provide high-throughput robotic pipetting for quantitative PCR and custom pipetting projects To offer instrumentation for western blot analysis including in-cell westerns of tissue cultures To offer the Seahorse XF24 Extracellular Flux Analyzer, an instrument that simultaneously measures the two major energy yielding pathways - aerobic respiration and glycolysis as a shared instrument To provide access to appropriate software analysis solutions for primer and probe design, sequence alignment, and data analysis for microarray and next-generation sequencing To develop new methods and protocols for analysis of gene expression including next-generation sequencing, quantitative PCR, microarray analysis, and DNA sequencing To provide instrumentation and expertise for quality control and accurate quantitation of RNA and DNA templates ¿ To reduce nutrition research-related costs by providing centralized genomics services and consultation at a fraction of the cost for commercial services or of maintaining instruments in individual investigators' laboratories. ¿ To provide training to principle investigators, research associates, post-doctoral researchers, and students in principles of DNA sequencing, quantitative PCR, RNA and DNA quality analysis, and next-generation sequencing ¿ To organize and make available training provided from outside sources ¿ To provide on-line and print educational and resource materials to increase institutional knowledge and speed the learning process to enhance the time required from experimental concept to data production and analysis ¿ To develop new collaborations for data analysis with Dr. Allison's group at UAB. Objectives Cell Biology and Bioimaging SubCore: ¿ To provide training on the proper use of our imaging, histology, and analytical flow instrumentation to any PBRC researcher or their staff at no charge ¿ To make high-end imaging, analytical, specimen preparation, and histological instrumentation accessible to the PBRC research community and to ensure the instrumentation is well maintained and operational to the best of our ability ¿ To provide consultation services as well as collaborate on specific scientific projects that make heavy use of CBBC equipment when possible ¿ To develop new methods or modify current techniques to increase the research potential of our imaging and analytical instrumentation ¿ To provide access to the necessary software for data analysis and assistance in its use for specific projects ¿ To make specific protocols, instructions for instrument use, links to free software for analysis, and relevant scientific articles relating to our instrumentation easily accessible to all via our web site ¿ To develop and offer high speed cell sorting capabilities to PBRC researchers ¿ To provide continued support for the development of CBBC staff skills through training via external or internal courses and/or scientific meetings

分子机理概述核心：在过去的五年中，NORC赠款期的分子机制发生了重大变化。这些变化代表了在基因组分析以及生物成像，细胞分类和传统组织学中引入主要技术引起的改进。为了适应这些变化并为更多的科学家提供更多服务，我们简化了领导力的组织，为将在每个子核心中建立技术的技术人员提供更多资金。在此期间，我们监视了核心服务的使用情况，并根据使用水平低，消除了细胞培养服务。这使我们能够重新分配资源并扩展细胞信号服务，现在称为细胞生物学和生物成像子核。这些更改在下面更详细地描述。基因组学子核的目标：执行DNA测序和碎片分析服务，包括双硫酸盐测序以及广泛的DNA类型的标准测序提供定量的PCR仪器，用于具有成本效益的共享用途，并支持实验设计，仪器和软件使用以及数据分析提供完整的微阵列分析服务，包括实验咨询，RNA质量控制，RNA的标签，微阵列芯片的杂交，扫描和数据生产提供下一代测序，包括咨询实验设计，赠款写作支持，图书馆创建服务或帮助，仪器运行和数据分析为定量PCR和自定义移量项目提供高通量机器人移液提供用于蛋白质印迹分析的仪器，包括组织培养的西部西部提供海马XF24细胞外通量分析仪，该仪器仅测量两个主要能量产生途径 - 有氧呼吸和糖酵解作为共享仪器为了提供适当的软件分析解决方案，用于底漆和探针设计，序列比对以及微阵列和下一代测序的数据分析开发新方法和方案，用于分析基因表达，包括下一代测序，定量PCR，微阵列分析和DNA测序提供仪器和专业知识，以质量控制和精确定量RNA和DNA模板 �通过提供集中的基因组服务和咨询，以商业服务的一小部分或在个别研究人员的实验室中维护工具来降低与营养研究相关的成本。 �为主要研究人员，研究助理，博士后研究人员和学生提供培训，以DNA测序，定量PCR，RNA和DNA质量分析以及下一代测序 �组织并提供从外部来源提供的培训 �提供在线和打印教育和资源材料以增加机构知识并加快学习过程，以加快从实验概念到数据生产和分析所需的时间 »与UAB的Allison博士小组开发新的合作，以进行数据分析。目标细胞生物学和生物成像子核心： �为任何PBRC研究人员或其员工免费提供有关我们的成像，组织学和分析仪器正确使用的培训 �为PBRC研究社区访问高端成像，分析，标本准备和组织学仪器，并确保仪器维护良好，并且能够尽最大的能力运行 �提供咨询服务并在特定的科学项目上进行协作，这些项目在可能的情况下大量使用CBBC设备 �开发新方法或修改当前技术以增加成像和分析仪器的研究潜力 �为数据分析提供必要的软件，以便在特定项目中使用 �为了制定特定的协议，仪器使用的说明，用于分析的免费软件的链接以及与我们的仪器有关的相关科学文章，可以通过我们的网站轻松访问我们的仪器 �为PBRC研究人员开发和提供高速细胞分类功能 �通过外部或内部课程和/或科学会议培训，为CBBC员工技能的发展提供了持续的支持