Animal Phenotyping Core

动物表型核心

• 批准号: 8490370
• 负责人: Martin Myers
• 金额: $ 4.5万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8490370
• 关键词: Animal Model; Animals; Body Composition; Cannulations; Cardiovascular system; Catheterization; Consultations; Development; Diagnostic; Diet; Disease; Education; Equipment; Ethers; Euglycemic Clamping; Event; Exercise; Genetic; Glucose Clamp; Goals; Individual; Intervention; Metabolic; Metabolic Diseases; Metabolism; Michigan; Molecular Genetics; Mus; Nutrient; Obesity; Obesity associated disease; Pharmaceutical Preparations; Phenotype; Prevention strategy; Process; Public Health; Rattus; Research; Research Personnel; Rodent; Rodent Model; Services; System; Techniques; Technology; Telemetry; Tissue Harvesting; combat; cost effective; design; food consumption; improved; insight; mouse model; new technology; novel; nutrition; respiratory; response; tool; treatment strategy

Animal Phenotyping Core A thorough understanding of the processes controlling the response to nutrients and of the mechanisms that contribute to obesity and related metabolic diseases is required if we are to effectively combat these conditions. The detailed analysis of animals with altered metabolism (e.g. due to dietary, molecular, genetic, or pharmacological manipulation) at a level that reveals basic underlying mechanisms of control requires specialized expertise and technology not normally available to individual investigators. Established in 2006, the goals of the MNORC Animal Phenotyping Core was are to provide state-of-the art equipment, services and consultative advice regarding the detailed metabolic phenotyping of rodent models of metabolic diseases. The Animal Phenotyping Core makes the metabolic analysis of rodent models of disease available, expeditious, affordable, effective, and convenient for individual investigators. In addition to providing education, consultation and advice regarding the analysis of rat and mouse models with altered metabolism, the Core provides phenotyping services on specialized equipment that it operates. Specifically, the core determines body composition and utilizes the CLAMS apparatus and other systems to examine metabolic rate, respiratory quotient, food consumption, and activity in rodent models of metabolic disease. The Core also examines the response to exercise and examines cardiovascular and ether parameters by telemetry in rodents. The Core performs hyperinsulinemic/euglycemic clamp studies including specialized analysis of metabolite storage and release in rats and mice, as well as providing catheterization/cannulation services and tissue harvesting in rodents. Thus, overall, the Animal Phenotyping Core will consultatively aid individual investigators in designing an appropriate experimental plan for the metabolic analysis of animal models relevant to obesity and then provide the tools and services necessary to effect this analysis. This research is relevant to public health because it will increase our understanding of the events that underlie the development of obesity and its complications, and hence will facilitate the development of improved diagnostic, prevention and treatment strategies.

动物表型核心 如果要有效地应对这些疾病，则需要对控制对养分的反应以及有助于肥胖和相关代谢疾病的机制的透彻理解。对具有改变代谢的动物的详细分析（例如，由于饮食，分子，遗传或药理学操纵）的详细分析，该水平揭示了控制的基本潜在机制，需要专业的专业知识和技术通常不可供个人研究人员使用。 MNORC动物表型核心的目标成立于2006年，是为了提供有关代谢疾病啮齿动物模型的详细代谢表型的最先进的设备，服务和咨询建议。动物表型核心使对个别研究人员的可用，快速，负担得起，有效且方便的疾病啮齿动物模型的代谢分析。除了提供有关用改变新陈代谢的老鼠和鼠标模型分析的教育，咨询和建议外，核心还为其运营的专用设备提供表型服务。具体而言，核心确定身体成分，并利用蛤s设备和其他系统来检查代谢疾病啮齿动物模型中的代谢率，呼吸商，食物消耗和活动。核心还检查了对运动的反应，并检查了啮齿动物中遥测的心血管和醚参数。该核心进行高胰岛素/尤格血糖夹的研究，包括对大鼠和小鼠的代谢产物储存和释放的专业分析，以及提供导管/插管服务以及啮齿动物中的组织收获。因此，总体而言，动物表型核心将协商个人研究人员设计适当的实验计划，以对与肥胖相关的动物模型的代谢分析，然后提供对该分析进行必要的工具和服务。这项研究与公共卫生有关，因为它将增加我们对肥胖发展及其并发症发展的事件的理解，因此将有助于改善诊断，预防和治疗策略的发展。