Mouse Physiology

小鼠生理学

• 批准号: 8469559
• 负责人: Lan Mao
• 金额: $ 21.78万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8469559
• 关键词: Animals; Biological Models; Blood Pressure; Calsequestrin; Cardiac; Catheters; Chronic; Conscious; Development; Echocardiography; Evaluation; Exercise; Genetic Models; Heart Hypertrophy; Heart failure; Hypertrophy; Knock-out; Laboratories; Measurement; Methodology; Methods; Modeling; Mus; Muscle Cells; Myocardial; Myocardial Infarction; Phenotype; Physiological; Physiology; Procedures; Running; Services; Stress; Surgical Models; Swimming; Techniques; Telemetry; Treadmill Tests; constriction; hemodynamics; in vivo; mouse model; muscle LIM protein; novel; overexpression; pressure; programs

1. Core Functions Mouse Physiology and Phenotyping: The small animal physiology core will provide two major services to all four of the projects in this PPG: 1) pathological and physiological models of cardiac hypertrophy and heart failure in mice, and 2) complete and comprehensive phenotyping of wild type and genetically modified mice. Mouse Models systems include: A) Mechanically- induced pressure overload hypertrophy and heart failure using the technique of transverse aortic constriction (TAC), both chronic and intermittent. B) Myocardial Infarction C) Genetic models of heart failure: muscle LIM protein knock out (MLP[-/-]) and calsequestrin (CSQ) overexpression. D) Swimming and running exercise. Mouse Phenotyping will include: A) An in-depth analysis of in vivo cardiac function by serial echocardiography in conscious mice. B) In vivo measurements of intrinsic myocardial contractility by pressure-volume (PV) loop analysis. C) In vivo end-systolic myocardial stress using stress-strain relations. D) In vivo PAR responsiveness by catheter derived hemodynamics. E) Invasive and non-invasive blood pressure recordings and exercise capacity by programmed treadmill testing. F) Ambulatory electrocardiographic telemetry. G) Ex vivo measurements of isolated myocyte contractility. The leaders of this core have been involved in the development of numerous surgical and genetic models of cardiac hypertrophy and heart failure. The Pi's laboratory has considerable expertise in a wide-ranging array of methodologies for the phenotyping of mice. The PI has also been involved in the development of a number of genetic and surgical models of heart failure. For example, microsurgical procedures and hemodynamic evaluation of pressure overload hypertrophy induced through transverse aortic constriction developed by the PI, is now a standard method used by many laboratories around the world.

1。核心功能 小鼠生理和表型： 小型动物生理核心将为本ppg的所有四个项目提供两项主要服务：1） 小鼠心脏肥大和心力衰竭的病理和生理模型，2）完整和 野生型和转基因小鼠的全面表型。 鼠标模型系统包括： a）使用横向技术机械诱导的压力超负荷肥大和心力衰竭 主动脉收缩（TAC），包括慢性和间歇性。 b）心肌梗塞 c）心力衰竭的遗传模型：肌肉lim蛋白敲除（MLP [ - / - ]）和calsequestin（CSQ） 过表达。 d）游泳和跑步运动。 鼠标表型将包括： a）在有意识的小鼠中串行超声心动图对体内心脏功能的深入分析。 b）通过压力卷（PV）环分析对内在心肌收缩性的体内测量。 c）使用应力 - 应变关系在体内终端性心肌压力。 d）导管衍生的血液动力学的体内反应性。 e）通过编程跑步机的侵入性和非侵入性血压记录和运动能力 测试。 f）卧床心电图遥测。 g）分离的肌细胞收缩性的体内测量。 该核心的领导者参与了许多手术和遗传模型的发展 心脏肥大和心力衰竭。 PI的实验室在广泛的范围内具有相当大的专业知识 小鼠表型的方法学阵列。 PI也参与了 心力衰竭的遗传和手术模型的数量。例如，显微手术程序和 通过横向主动脉收缩引起的压力超负荷肥大的血液动力学评估 由PI开发，现在是世界上许多实验室使用的标准方法。