Core B -Comprehensive Mouse Physiology and Phenotyping

核心 B - 综合小鼠生理学和表型分析

• 批准号: 9118292
• 负责人: Lan Mao
• 金额: $ 25.18万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9118292
• 关键词: Adrenergic Receptor; Animal Model; Animals; Blood Pressure; Cardiac; Catheters; Conscious; Data; Development; Echocardiography; Electrocardiogram; Evaluation; Exercise; Genetic Models; Goals; Growth; Heart; Heart Hypertrophy; Heart failure; Hypertrophy; Laboratories; M-Mode Echocardiography; Measurement; Measures; Medicine; Methodology; Methods; Modeling; Molecular; Monitor; Mus; Muscle Cells; Myocardial Ischemia; Operative Surgical Procedures; Pathologic; Phenotype; Physiological; Physiology; Procedures; Protocols documentation; Receptor Signaling; Research Personnel; Running; Services; Signal Pathway; Stress; Stretching; Surgical Models; Swimming; Training; constriction; exercise capacity; hemodynamics; in vivo; mechanical pressure; member; molecular targeted therapies; mouse model; novel; physiologic model; pressure; professor; rat Ran 2 protein

SUMMARY OF WORK: Core B – Comprehensive Mouse Physiology and Phenotyping Comprehensive mouse physiology and mouse phenotyping including surgical models of pressure overload to induce cardiac hypertrophy and heart failure. The PI's laboratory has considerable expertise in a wide-range of methodologies for the phenotyping of mice and will provide Core service to members of the PPG. The PI has also been involved in the development of numerous genetic and surgical models of heart failure. For example, microsurgical procedures and hemodynamic evaluation of pressure overload cardiac hypertrophy induced through transverse aortic constriction was developed by the PI, and is now considered a routine procedure in many labs around the world. Transthoracic 2D guided M-mode echocardiography, hemodynamic and contractile function by pressure-volume loops and stress strain analyses in mice are standard procedures, many which were developed in the PI's laboratory. Exercise training by both swimming and wheel running for mice are methods currently being used.

工作摘要：核心B - 全面的鼠标生理和表型 全面的小鼠生理学和小鼠表型，包括压力超负荷的手术模型 诱导心脏肥大和心力衰竭。 PI的实验室考虑了广泛的专业知识 小鼠表型的方法，将为PPG成员提供核心服务。 pi 还参与了许多心力衰竭的遗传和外科手术模型的发展。为了 例如，压力超负荷心脏肥大的显微外科手术和血液动力学评估 PI通过横向主动脉收缩诱导的诱导，现在被认为是常规 世界许多实验室的程序。经胸2D引导M模式超声心动图，血流动力学 在小鼠中，压力卷回路和应力应变分析是标准程序，而收缩功能是标准程序， 许多是在PI实验室中开发的。游泳和轮子跑步的运动训练 小鼠是当前正在使用的方法。