Animal Models Core

动物模型核心

• 批准号: 8529524
• 负责人: Susan Bailey Gurley
• 金额: $ 32.38万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8529524
• 关键词: Adoption; Affect; Animal Model; Animals; Area; Atherosclerosis; Awareness; Biological Assay; Blood Pressure; Blood Vessels; Cardiac; Cardiology; Cardiovascular Diseases; Cardiovascular Physiology; Cardiovascular system; Chronic Kidney Failure; Clinical; Communities; Complex; Conscious; Core Facility; Data; Data Analyses; Data Collection; Disease model; End stage renal failure; Etiology; Experimental Designs; Experimental Models; Functional disorder; Genomics; Heart; Heart Diseases; Heart failure; Human; Human Genetics; Human Resources; Hypertension; Image; Individual; Institution; International; Investigation; Kidney; Kidney Diseases; Kidney Transplantation; Laboratories; Link; Measures; Metabolic; Methods; Modeling; Mus; Nephrology; Outcome; Patients; Phenotype; Physiological; Physiological Processes; Procedures; Research; Research Personnel; Risk; Robotics; Services; Study models; Technical Expertise; Techniques; Technology; Testing; Training; Transplantation; Vascular Diseases; Work; Zebrafish; base; cardiovascular disorder risk; clinically relevant; cost; design; genetic manipulation; genetic variant; human data; human disease; in vivo; interest; mortality; mouse model; multidisciplinary; pressure; research study; skills; tool; translational study

Chronic kidney disease (CKD) is associated with a marked increase in risk for cardiovascular disease (CVD), such that patients with CKD are more likely to die from CVD than to progress to end-stage renal disease. Hypertension (HTN) amplifies risk for CVD and affects more than 1 billion people worldwide. However, the causes of HTN cannot be determined in most patients and the mechanisms underlying the complex interactions among the kidney, HTN and cardiovascular disease and subsequent risk for mortality are not clear. We suggest that precise definition of these mechanisms will require multi-disciplinary teams with expertise in renal and cardiovascular systems. Accordingly, our objective is to provide a panel of sophisticated tools to support and facilitate multi-disciplinary, basic discovery research in animal models on the unique links relating the kidney, cardiovascular disease and HTN. The Core is configured to provide access to a range of experimental models of kidney, heart and vascular diseases, and Core personnel have comprehensive expertise in utilizing mouse models of CKD, CVD, and HTN, as well as functional kidney and cardiovascular screens in non-mammalian models. The Core will serve kidney or cardiovascular investigators who may be working in isolation to raise their awareness of the clinical impact of the heart-kidney axis and to provide access to models and techniques that can entice them to expand their efforts into our broader area of thematic interest. We will provide comprehensive phenotyping services for kidney, blood pressure and other cardiovascular functions. The scope of the Core will be Regional, National and International. Core staff will assist in experimental design as well as data collection and analysis. Capabilities of the Core will also be useful for determining functional relevance of genetic variants discovered in studies based in the Renal Genomics Core as well as providing preliminary data for human studies in the Clinical and Translational Core. The Animal Models Core has four specific aims: (1) to provide access to mouse models of CKD, HTN, and CVD; (2) to provide state-of-the-art kidney and cardiovascular phenotyping in mice; (3) to generate models of kidney cross-transplantation for distinguishing renal and systemic contributions to physiologic, metabolic and/or immunological functions in mice; and (4) to provide rapid screens in zebrafish for genetic variants with functional effects in kidney and/or cardiovascular system.

慢性肾脏疾病（CKD）与心血管疾病（CVD）的风险显着增加有关，因此CKD患者更有可能死于CVD，而不是发展为终末期肾脏疾病。高血压（HTN）扩大了CVD的风险，并影响全球超过10亿人。但是，在大多数患者中无法确定HTN的原因，并且肾脏，HTN和心血管疾病之间复杂相互作用的机制以及随后死亡率的风险尚不清楚。我们建议对这些机制的精确定义需要具有肾脏和心血管系统专业知识的多学科团队。因此，我们的目标是提供一系列精致的工具，以支持和促进动物模型中的多学科基本发现研究，该研究与肾脏，心血管疾病和HTN有关的独特联系。该核心的配置为提供肾脏，心脏和血管疾病的一系列实验模型，并且核心人员在利用CKD，CVD和HTN的小鼠模型以及功能性肾脏和心血管屏幕中具有全面的专业知识。核心将为肾脏或心血管调查员提供服务，他们可能会孤立地工作，以提高他们对心脏 - 基德尼轴的临床影响的认识，并提供对模型和技术的访问，以吸引他们将其努力扩展到我们更广泛的主题兴趣领域。我们将为肾脏，血压和其他心血管功能提供全面的表型服务。核心的范围将是区域，国家和国际。核心人员将协助实验设计以及数据收集和分析。核心的能力也将有助于确定基于肾脏基因组核心的研究中发现的遗传变异的功能相关性，并为临床和转化核心的人类研究提供初步数据。动物模型核心具有四个特定的目的：（1）提供对CKD，HTN和CVD的小鼠模型的访问； （2）在小鼠中提供最先进的肾脏和心血管表型； （3）生成肾脏交叉移植模型，以区分小鼠的生理，代谢和/或免疫功能的肾脏和全身贡献； （4）在斑马鱼中提供快速筛选，以使其在肾脏和/或心血管系统中具有功能效应的遗传变异。