Molecular Photoacoustic Imaging for the Detection of Sentinel Lymph Node Metastas

用于检测前哨淋巴结转移的分子光声成像

• 批准号: 8317207
• 负责人: Geoffrey P. Luke
• 金额: $ 3.85万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-01 至 2015-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8317207
• 关键词: Academia; Algorithms; Caliber; Cancer Biology; Cancer Model; Cancer Patient; Cancerous; Cells; Clinical; Coupled; Coupling; Detection; Diagnosis; Endocytosis; Endosomes; Enrollment; Epidermal Growth Factor Receptor; Epithelial; Epithelial Neoplasms; Ethics; Exhibits; Frequencies; Goals; Gold; Histology; Image; Imaging Techniques; Imaging technology; In Vitro; Incubated; Injection of therapeutic agent; Knowledge; Lasers; Lymphatic; Lymphatic System; Malignant Neoplasms; Measures; Mediating; Medical; Mentors; Methods; Micrometastasis; Modeling; Molecular; Mus; Nanosphere; Neoplasm Metastasis; Patients; Physiologic pulse; Protocols documentation; Public Health; Research; Research Personnel; Resected; Resolution; Sampling; Sentinel; Sentinel Lymph Node; Squamous cell carcinoma; System; Technical Expertise; Technology; Testing; Therapeutic; Time; Training; Ultrasonography; Writing; absorption; bioimaging; cancer cell; cancer imaging; career; cost; design; experience; image processing; improved; in vivo; lymph nodes; mouse model; nanobiotechnology; nanosecond; nanosensors; outcome forecast; overexpression; plasmonics; pre-clinical; programs; receptor; receptor mediated endocytosis; research clinical testing; skills; spectroscopic imaging

DESCRIPTION (provided by applicant): In cancer patients, determination of whether a malignancy has spread is the single most important factor used to develop a therapeutic plan and to predict prognosis. In most cases, cancer cells initially spread through regional lymph nodes. Therefore, clinical evaluation for the presence of regional lymph node metastases is of paramount importance. Unfortunately, there are no real-time, non-invasive clinical methods that can reliably detect and diagnose micrometastases in lymph nodes. Thus, there is an urgent clinical need for an imaging technique that is widely available, is non-invasive and simple to perform, is safe, and can reliably detect and adequately diagnose lymph node micrometastases in real time. The overall goal of our research program is to develop an advanced, in vivo, noninvasive, molecular specific imaging technology, i.e., integrated ultrasound and photoacoustic imaging combined with targeted plasmonic nanosensors, capable of immediate and accurate assessment of sentinel lymph node micrometastases in real time. The first aim of the project is to develop a combined photoacoustic and ultrasound imaging system and protocol. The system will consist of a high frequency ultrasound machine coupled with a pulsed nanosecond tunable laser. The second aim is to develop spectroscopic image processing algorithms to detect sentinel lymph node metastasis after the injection of plasmonic nanosensors. The imaging system and algorithms will be tested on in vitro cell samples of nanosensors incubated with normal and cancerous cells. The final aim is to use the imaging system and spectroscopic algorithms to detect lymph node micrometastases in a murine cancer model in vivo. The imaging results will be correlated with histology of the resected lymph nodes. The training plan proposed to accomplish these goals has been designed to establish trainee mentors with specific technical expertise. The selected mentors are experts in ultrasound and photoacoustic imaging and image processing. The PI will enroll in formal training in the ethical conduct of research and in training specific to his research field, to prepare him for independent research. PUBLIC HEALTH RELEVANCE: In cancer patients, the determination of the spread of malignancy is the single most important factor to develop a therapeutic plan and predict prognosis. In most cases, cancer cells initially spread through regional lymph nodes. Thus, a technology such as molecular specific ultrasound and photoacoustic lymphatic imaging, capable of in-vivo, noninvasive and accurate assessment of regional metastases in real time, can simplify and improve management of patients with epithelial malignancies, significantly improve public health, and reduce medical costs.

描述（由申请人提供）：在癌症患者中，确定恶性肿瘤是否已扩散是用于制定治疗计划和预测预后的最重要因素。在大多数情况下，癌细胞最初通过区域淋巴结扩散。因此，临床评估区域淋巴结转移的存在至关重要。不幸的是，没有实时、非侵入性的临床方法可以可靠地检测和诊断淋巴结中的微转移。因此，临床迫切需要一种可广泛使用、非侵入性、操作简单、安全、能够可靠地实时检测和充分诊断淋巴结微转移的成像技术。我们研究计划的总体目标是开发一种先进的体内、无创、分子特异性成像技术，即集成超声和光声成像与靶向等离子体纳米传感器相结合，能够实时、准确地评估前哨淋巴结微转移。该项目的首要目标是开发组合光声和超声成像系统和协议。该系统将由高频超声机和脉冲纳秒可调谐激光器组成。第二个目标是开发光谱图像处理算法来检测注射等离子体纳米传感器后的前哨淋巴结转移。成像系统和算法将在与正常细胞和癌细胞一起孵育的纳米传感器的体外细胞样本上进行测试。最终目标是使用成像系统和光谱算法来检测体内小鼠癌症模型中的淋巴结微转移。成像结果将与切除的淋巴结的组织学相关。为实现这些目标而提出的培训计划旨在建立具有特定技术专长的实习导师。选定的导师是超声和光声成像以及图像处理领域的专家。 PI 将参加有关研究道德行为的正式培训以及针对其研究领域的培训，为他进行独立研究做好准备。 公共卫生相关性：在癌症患者中，确定恶性肿瘤的扩散是制定治疗计划和预测预后的最重要因素。在大多数情况下，癌细胞最初通过区域淋巴结扩散。因此，分子特异性超声和光声淋巴成像等技术能够对区域转移进行体内、无创、准确的实时评估，可以简化和改善上皮恶性肿瘤患者的治疗，显着改善公众健康，并减少医疗费用。成本。