Re-wiring of gene expression in the meiotic state

减数分裂状态下基因表达的重新布线

• 批准号: 8451428
• 负责人: BRUCE Bruce FUTCHER
• 金额: $ 143.77万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2015-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8451428
• 关键词: Antisense RNA; Cells; Chromatin; Core Facility; Fission Yeast; Gene Expression; Gene Expression Regulation; Genes; Meiosis; Play; RNA Processing; Research Project Grants; Role; Saccharomyces cerevisiae; Vegetative States; histone modification; novel; programs; transcription factor

DESCRIPTION (provided by applicant): Overview - This Program Project explores the idea that when a cell enters the meiotic state, it undergoes a radical re-wiring of gene expression programs. A more conservative view was that meiotic cells are broadly similar to vegetative cells, but express additional, meiosis-specific genes. We explore a more radical view in which even genes that are expressed in both vegetative and meiotic cells are expressed in a different fashion, regulated by different transcription factors in meiotic cells. Furthermore meiotic cells may use a larger repertoire of mechanisms to control gene expression than vegetative cells. In short, when cells differentiate from the vegetative state into the meiotic state, their gene expression programs are extensively re-wired. We will study this re-wiring, and novel, meiosis-specific regulatory mechanisms. The first three projects use S. cerevisiae, and focus on regulatory mechanisms in early, middle, and late meiosis, respectively. The fourth project uses S. pombe, and focuses on novel meiotic regulatory mechanisms. Projects. 1. Wiring of Gene Expression in the Early Meiotic Program (Dr. Futcher) 2. Meiotic Cdc7 switches and substrates, and the activation of Ndt80 (Dr. Hollingsworth) 3. Histone modification and Chromatin Switches in the Meiotic state (Drs. Neiman and Sternglanz) 4. Novel mechanisms of gene regulation in Meiosis: Antisense RNAs and RNA processing. (Dr. Leatherwood) The research Projects will be supported by a Microarray Core Facility, which will play a crucial role in all four projects.

描述（由申请人提供）：概述 - 该程序项目探讨了一个观念，即当一个单元进入减数分裂状态时，它会对基因表达程序进行根本性重新培训。一个更保守的观点是，减数分裂细胞与营养细胞大致相似，但表达了额外的减数分裂特异性基因。我们探讨了一种更加激进的观点，即在营养和减数分裂细胞中表达的基因也以不同的方式表达，受减数分裂细胞中不同转录因子的调节。此外，减数分裂细胞可能使用更大的机制来控制基因表达，而不是营养细胞。简而言之，当细胞从营养状态区分到减数分裂状态时，它们的基因表达程序被广泛重新连接。我们将研究这种重新布局，新颖的减数分裂特异性调节机制。前三个项目分别使用酿酒酵母，并分别专注于早期，中期和晚期减数分裂的调节机制。第四个项目使用S. pombe，专注于新型的减数分裂调节机制。 项目。 1。早期减数分裂计划中基因表达的接线（Futcher博士） 2。减数分裂CDC7开关和底物，以及NDT80的激活（Hollingsworth博士） 3。减数分裂状态的组蛋白修饰和染色质切换（Neiman和Sternglanz博士） 4。减数分裂中基因调节的新型机制：反义RNA和RNA处理。 （博士 莱特伍德） 研究项目将得到微阵列核心设施的支持，该设施将在所有四个项目中发挥关键作用。