Microarray Core

微阵列核心

• 批准号: 7917082
• 负责人: BRUCE Bruce FUTCHER
• 金额: $ 17.91万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2015-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7917082
• 关键词: Bioinformatics; Cataloging; Catalogs; Cells; Core Facility; Custom; Data; Databases; Freezing; Gene Expression; Home environment; Instruction; Label; Methods; Phase; Process; RNA; Research Personnel; Services; computerized data processing; member; programs

The Microarray Core Facility will provide microarray services, and bioinformatic services, to the members of the Program Project. The microarray services will include home-made gene expression microarrays (although we are phasing this out); catalogue and custom Agilent microarrays; and catalogue Affymetric microarrays, for both gene expression and ChlP-Chip analysis. Typically the Microarray Core Facility will receive a pellet of frozen cells from the investigator. The frozen pellet will be processed to yield RNA, and this RNA will be labeled and hybridized to a micorarray. Data will be processed and loaded to an SQL database. Investigators will access this partially processed data in the database. Thus the Core facility can handle all steps from the frozen cell pellet to the storage of partially processed data. All members of the Program Project will use this facility heavily, and the commonality of methods and analysis, and the presence of all relevant data in the same database, will greatly increase our ability to interpret the data.

微阵列核心设施将为该计划项目的成员提供微阵列服务和生物信息服务。微阵列服务将包括自制基因表达微阵列（尽管我们正在逐步淘汰）；目录和定制安捷伦微阵列；和目录 Affymetric 微阵列，用于基因表达和 ChlP 芯片分析。通常，微阵列核心设施将从研究者处接收冷冻细胞颗粒。冷冻沉淀将被加工以产生 RNA，该 RNA 将被标记并与微阵列杂交。数据将被处理并加载到 SQL 数据库中。研究人员将访问数据库中部分处理的数据。因此，核心设施可以处理从冷冻细胞沉淀到部分处理数据存储的所有步骤。该计划项目的所有成员都将大量使用该设施，方法和分析的通用性以及所有相关数据都存在于同一数据库中，将大大提高我们解释数据的能力。