Molecular Definition of Brain Circuits Controlling Addiction

控制成瘾的大脑回路的分子定义

基本信息

批准号：
8551017
负责人：
NATHANIEL HEINTZ
金额：
$ 137.68万
依托单位：
ROCKEFELLER UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-07-01 至 2018-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8551017
关键词：
Addictive Behavior Advisory Committees Animal Model Behavioral Belief Biological Models Brain Cells Chronic Cocaine Core Facility Data Data Analyses Data Set Development Drug abuse Ensure Epigenetic Process Event Funding Gene Expression Alteration Generations Genetic Models Individual Informatics Investigation Laboratories Maps Mediating Methodology Methods Molecular Molecular Analysis Molecular Genetics National Institute of Drug Abuse Nature Nicotine Opioid Pharmaceutical Preparations Pilot Projects Refractory Research Research Personnel Resources Role Scientist Self Administration Services Site Stimulus Substance abuse problem Techniques Technology Training Transgenic Mice Transgenic Organisms United States National Institutes of Health Withdrawal addiction cell type comparative computing resources drug of abuse drug withdrawal epigenome in vivo investigator training meetings neural circuit new technology novel strategies novel therapeutic intervention operation outreach programs public health relevance ranpirnase recombinase research study response technology development

项目摘要

DESCRIPTION (provided by applicant): The NIDA P30 "Center for Molecular and Epigenetic Research of Cell Types Mediating Addictive Behaviors" proposed here will provide to NIH investigators state of the art resources, technologies and expertise that will significantly enhance the programs of each of the participating laboratories, and enable development of additional advanced methodologies that are of general utility for the investigation of mechanisms of drug abuse. To meet these objectives, the Center will be organized into four Cores: The Administrative Core will organize and manage all programmatic and fiscal operations of the Center, including outreach to each of the Center investigators, management of quarterly online meetings of the External Advisory Committee, and hosting of Center investigators or their staff during the conduct of on-site experiments. The BAC Recombineering and Transgenic Targeting Core will generate, characterize and distribute specialized transgenic lines for advanced molecular studies of cell types contributing to addiction circuitry; The Molecular and Epigenetic Profiling Core will provide specialized behavioral and experimental services for the conduct of TRAP translational profiling and epigenetic mapping studies in the context of chronic drug treatment, drug self-administration, and drug withdrawal; The Molecular Informatics Core will provide facilities and expertise to help investigators collect, store and analyze the very large amounts of data that will result from their microarray, TRAPseq, epigenome mapping and ChlPseq studies. The Center will also support a small number of Pilot Projects aimed at developing new technologies that will advance discovery of molecular mechanisms that contribute to drug abuse, or extension of existing approaches to mammalian model systems that have been refractory thus far to modern molecular genetic approaches The outstanding scientists participating in the Center share the belief that: it is beyond the capacity of any participating laboratory to generate the large number of specialized transgenic lines required for comprehensive genetic, molecular and epigenetic analysis of addiction circuits; and that a Center in which their laboratories can apply advanced methods for analysis of addiction circuits will significantly advance their research programs.

描述（由申请人提供）：NIDA P30“介导的成瘾行为的细胞类型的分子和表观遗传研究中心”将为NIH研究人员提供艺术资源，技术和专业知识的状态，这些水平，技术和专业知识将显着增强每种参与实验室的计划，并能够开发出对一般药物的其他高级药物的开发。为了实现这些目标，该中心将分为四个核心：行政核心将组织和管理该中心的所有程序和财政运营，包括向每个中心调查人员进行宣传，外部咨询委员会的季度在线会议管理，并在现场实验中举办中心调查人员或其员工的主持人。 BAC重组和转基因靶向核心将产生，表征和分布专门的转基因线，用于对成瘾回路的细胞类型的高级分子研究；分子和表观遗传分析核心将在慢性药物治疗，药物自我给药和戒断药物的背景下，为陷阱翻译分析和表观遗传学映射研究提供专门的行为和实验服务；分子信息学核心将提供设施和专业知识，以帮助研究人员收集，存储和分析其微阵列，Trapseq，Epegenome映射和CHLPSEQ研究所产生的大量数据。该中心还将支持少量的试点项目，旨在开发新技术，以提高发现有助于滥用药物的分子机制，或者将现有方法扩展到迄今为止对现代分子遗传学方法难治的哺乳动物模型系统的现有方法扩展到现代分子遗传学方法，使杰出的杰出科学家参与该中心的能力超出了：它与该中心共享：它与之共享：它是：它是：它与之共享：它是：它与之共享。在任何参与的实验室中生成综合遗传，分子和表观遗传分析所需的大量专门转基因线；而且，他们的实验室可以应用高级方法进行成瘾电路的中心将大大提高其研究计划。