Long-term In Vivo Monitoring of Neuromuscular Performance in Mice

小鼠神经肌肉性能的长期体内监测

基本信息

批准号：
8627916
负责人：
Pedro Irazoqui
金额：
$ 26.4万
依托单位：
JACKSON LABORATORY
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-09-01 至 2015-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8627916
关键词：
Action Potentials Amyotrophic Lateral Sclerosis Anesthesia procedures Animal Model Animals Appearance Biocompatible Chronic Clinic Collaborations Collection Communities Computers Conscious Data Defect Denervation Development Devices Diagnosis Diagnostic Disease Disease Progression Electromyography Evaluation Evaluation Studies Event Face Funding Gait Gene Mutation Genes Genetic Goals Human Implant Individual Measurement Measures Methods Modeling Molecular Genetics Monitor Morphologic artifacts Motor Motor Neurons Movement Mus Muscle Mutation Nerve Neuromuscular Diseases Output Pathology Patients Performance Phenotype Prosthesis Protocols documentation Reflex action Relative (related person)Research Research Personnel Resolution Superoxide Dismutase Technology Technology Transfer Testing Time Transgenes Universities Validation Wireless Technology Work awake base clinically relevant congenic design electrical measurement implantable device improved in vivo meetings mouse model mutant neuromuscular novel public health relevance reinnervation research and development response tool

项目摘要

PROJECT SUMMARY/ABSTRACT Our ability to investigate the genetic basis of neuromuscular disease has entered a new era as advances in sequencing allow more rapid identification of underlying mutations. Mutations identified in patients can be moved quickly to model organisms, such as mice, for the detailed mechanistic studies not possible in patients. The number of available mouse models of neuromuscular disease is increasing constantly, as is the repertoire of available molecular genetics tools. By comparison, there is a relative lack of sophisticated tools for in vivo phenotypic characterization in mice with neuromuscular disease. In the clinic, electromyography (EMG) and nerve action potentials (NAP) data are routinely collected from patients for diagnosis and research. These measures of neuromuscular performance are particularly useful because they can be repeated and used to monitor progression or response to treatment over time. We propose to develop similar capabilities in mice by refining existing implantable technology that will allow longitudinal recording of EMG and NAP in untethered, freely moving mice.

项目摘要/摘要随着进步的发展，我们研究神经肌肉疾病遗传基础的能力已经进入了一个新时代测序可以更快地识别潜在的突变。患者发现的突变可能是迅速移动到建模小鼠等生物，以便患者无法进行详细的机械研究。神经肌肉疾病的可用小鼠模型的数量不断增加，曲目也是如此可用的分子遗传学工具。相比之下，相对缺乏体内体内的复杂工具神经肌肉疾病的小鼠表型表征。在诊所，肌电图（EMG）和神经动作电位（NAP）数据通常是从患者中进行诊断和研究的。这些神经肌肉性能的度量特别有用，因为它们可以重复并用于随着时间的流逝，监测对治疗的进展或反应。我们建议通过完善现有的可植入技术，该技术将允许在不受限制的emg和nap中纵向记录自由移动老鼠。