Endocrinology of Mosquito Reproduction

蚊子繁殖的内分泌学

基本信息

批准号：
8181109
负责人：
MARK R BROWN
金额：
$ 31.28万
依托单位：
UNIVERSITY OF GEORGIA
依托单位国家：
美国
项目类别：
财政年份：
1992
资助国家：
美国
起止时间：
1992-07-01 至 2016-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8181109
关键词：
Address Aedes Affect Affinity Amino Acids Anopheles gambiae Area Arthropod Vectors Award Binding Biological Biology Blood Brain Complex Cues Culicidae Development Digestion Disabled Persons Disease Ecdysteroids Egg Yolk Proteins Endocrinology Evolution Exhibits Family member Fat Body Feeding behaviors Female Foundations Goals Hormone Receptor Hormones Human Individual Insecta Insulin Insulin Receptor Insulin Signaling Pathway Laboratories Link Longevity Measures Mediating Midgut Mosquito Control Neurohormones Nutrient Ochlerotatus Oocytes Outcome Ovary Parasites Peptides Process Production Protein Biosynthesis Relative (related person)Reproduction Role Signal Transduction Sirolimus Translating Vitellogenins Work absorption computerized data processing disease transmission disorder control egg feeding inhibitor/antagonist insight knowledge base meetings novel novel strategies pathogen receptor binding research study transmission process uptake vector

项目摘要

DESCRIPTION (provided by applicant): Mosquitoes are well recognized as the most important arthropod vectors of disease-causing pathogens. This is because most species are anautogenous and must feed on the blood of a human or other vertebrate to produce eggs. Multiple cycles of blood feeding and egg development in turn favors frequent contacts with hosts, which makes mosquitoes ideal vectors for pathogen transmission. Since disruption of egg production also disables pathogen transmission, understanding the reproduction of anautogenous mosquitoes is an important area of study. This application is a renewal request to continue our work on how egg development is regulated. Our current award focuses on Aedes aegypti, which is the species we best understand how egg maturation is regulated. Our studies indicate that two neurohormones, ovary ecdysteroidogenic hormone (OEH) and insulin-like peptides (ILPs) from the brain, function as key regulators of the egg development process. However, we also have found that OEH but not ILPs is also able to stimulate egg formation in the absence of blood feeding. To advance these results, we need to better understand OEH function in Ae. aegypti, other anautogenous species like Anopheles gambiae, and non-blood feeding autogenous species like. Our Specific Aims to meet these goals are: 1. Characterize OEH mediated nutrient mobilization and egg maturation in Ae. aegypti. 2. Assess the roles of OEH and ILPs in egg development of An. gambiae and O. atropalpus. 3. Assess the effects of OEH, ILP3, rapamycin and insulin receptor inhibitor PQIP in disabling mosquito reproduction. Expected outcomes of our work will include an enhanced understanding of how egg production is regulated in anautogenous and autogenous species. Our study will also provide important insights into how egg development can be disabled. PUBLIC HEALTH RELEVANCE: Mosquitoes are the most important arthropod vectors of disease-causing pathogens. Our work will help us understand the mechanisms that control blood feeding and egg production in mosquitoes, which are critical issues in transmission of disease-causing parasites. This will provide the foundation for development of new approaches for controlling mosquito reproduction and reducing disease transmission.

描述（由申请人提供）：蚊子被广泛认为是最重要的致病病原体节肢动物载体。这是因为大多数物种都是厌食的，必须以人或其他脊椎动物的血液为食来产生卵。多个循环的血液喂养和卵发育又有利于与宿主的频繁接触，这使蚊子成为病原体传播的理想媒介。由于产生卵的破坏也可以禁用病原体的传播，因此了解肛门蚊子的繁殖是一个重要的研究领域。该申请是一个续签请求，以继续我们在如何调节鸡蛋开发方面的工作。我们目前的奖项重点介绍了埃及埃及的奖项，这是我们最好理解如何调节卵的物种。我们的研究表明，来自大脑的两种神经激素（OEH）和胰岛素样肽（ILP）是卵发育过程的关键调节剂。但是，我们还发现，在没有血液喂养的情况下，OEH而不是ILP也能够刺激卵的形成。为了推进这些结果，我们需要更好地了解AE中的OEH功能。埃及，其他卵巢源性物种，如冈比亚的巨蛋和非血液喂养自源物种。我们实现这些目标的具体目的是：1。表征OEH介导的营养动员和AE中的鸡蛋成熟。埃及。 2。评估OEH和ILP在An的卵发育中的作用。冈比亚和O. Atropalpus。 3。评估OEH，ILP3，雷帕霉素和胰岛素受体抑制剂PQIP对蚊子繁殖的影响。我们工作的预期结果将包括对在肛门和自源物种中如何调节卵的产生的了解。我们的研究还将提供有关如何残疾卵发育的重要见解。公共卫生相关性：蚊子是致病病原体的最重要的节肢动物载体。我们的工作将帮助我们了解控制蚊子中血液喂养和产卵的机制，这是引起疾病寄生虫传播的关键问题。这将为开发用于控制蚊子繁殖和减少疾病传播的新方法的基础。