LONGITUDINAL 1H NMR SPECTROSCOPY OF MOUSE HD

小鼠 HD 纵向 1H NMR 波谱

• 批准号: 8170446
• 负责人: JANET M DUBINSKY
• 金额: $ 2.57万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170446
• 关键词: Biochemical; Brain Diseases; Brain region; Computer Retrieval of Information on Scientific Projects Database; DNA; Defect; Funding; Genes; Grant; Human; Huntington Disease; Inherited; Institution; Methods; Molecular; Monitor; Mus; NMR Spectroscopy; Nerve Degeneration; Neurodegenerative Disorders; Process; Research; Research Personnel; Resources; Source; Transgenic Mice; United States National Institutes of Health; brain cell; in vivo; magnetic field; mouse model; neurochemistry; programs

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this project is to investigate the neurochemical changes occurring during the progression of the Huntington's disease (HD) using transgenic mouse models. Huntington's disease is an inherited neurodegenerative disease resulting from genetically programmed degeneration of brain cells in certain brain regions. Genetically manipulated mice, which DNA contain the defected human gene, are used to mimic this human brain disease. In vivo 1H NMR spectroscopy at high magnetic fields is used for a non-invasive quantification of metabolites from selected mouse brain regions. Non-invasive character of this method enables a longitudinal monitoring of biochemical changes underlying the neurodegeneration. The aim of this project is a better understanding of the mechanisms and processes of the neurodegeneration in HD on the molecular level.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 该项目的目的是利用转基因小鼠模型研究亨廷顿病 (HD) 进展过程中发生的神经化学变化。亨廷顿舞蹈症是一种遗传性神经退行性疾病，由某些大脑区域的脑细胞基因程序性退化引起。基因改造小鼠的 DNA 中含有缺陷的人类基因，被用来模拟这种人类大脑疾病。高磁场下的体内 1H NMR 光谱用于对选定的小鼠大脑区域的代谢物进行非侵入性定量。该方法的非侵入性特征使得能够对神经变性背后的生化变化进行纵向监测。该项目的目的是在分子水平上更好地了解 HD 神经变性的机制和过程。