ULTRASTRUCTURAL ANALYSIS OF MEMBRANE-ASSOCIATED COMPLEXES IN S CEREVISIAE

酿酒酵母膜相关复合物的超微结构分析

• 批准号: 8362742
• 负责人: Jeremy W. Thorner
• 金额: $ 1.96万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362742
• 关键词: Cell division; Cell membrane; Cells; Complex; Cytokinesis; Dendritic Spines; Development; Eukaryota; Eukaryotic Cell; Filament; Fingers; Fluorescence; Fluorescence Microscopy; Funding; GTP-Binding Proteins; Genes; Genome; Grant; Homo sapiens; Image; Location; Membrane; National Center for Research Resources; Neurons; Principal Investigator; Proteins; Research; Research Infrastructure; Resources; Saccharomyces cerevisiae; Source; Staging; United States National Institutes of Health; X-Ray Tomography; Yeasts; cell type; cost; paralogous gene; polymerization; retinal rods

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Septins are GTP-binding proteins encoded by paralogous genes found in the genomes of nearly every eukaryote examined. These proteins are involved in the remodeling of and demarcation of compartments within the plasma membrane, for example during cytokinesis (Finger, 2005) and in the maturation of dendritic spines in neurons (Caudron and Barral, 2009). Unicellular eukaryote Saccharomyces cerevisiae (baker's yeast) encodes seven septin genes, and Homo sapiens fourteen (Pan et al., 2007). Any given eukaryotic cell type expresses multiple sets of septins, often in a cell type- and developmental stage-specific manner. In all cases known, the protein products of those genes associate in a defined order and assemble into a linear array (McMurray and Thorner, 2008a; Weirich et al., 2008). Moreover, the resulting linear hetero-oligomer ("rod") is a building block that has the capacity to undergo end-on-end polymerization, thereby forming filaments. It is known that these filaments are involved with cell division, but fluorescence microscopy alone has not revealed sufficient details to thoroughly understand the exact mechanism. We propose to use correlated fluorescence and x-ray imaging to determine the location of each of the septins in the yeast, Saccharomyces cerevisiae.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 Septins是GTP结合蛋白，该蛋白几乎由所检查的每个真核生物的基因组中发现。这些蛋白质参与了质膜内隔室的重塑和分界，例如 细胞因子（手指，2005）和神经元中树突状棘的成熟（Caudron和Barral，2009）。酿酒酵母单细胞真核生物糖果（Baker's酵母）编码七个九月基因，而Homo Sapiens十四（Pan等，2007）。任何给定的真核细胞类型通常以细胞类型和发育阶段特异性的方式表达多组septins。在所有已知的情况下，这些基因的蛋白质产物以定义的顺序相关并组装成线性阵列（McMurray和Thorner，2008a； Weirich等，2008）。此外，由此产生的线性杂音寡聚物（“杆”）是一个有能力进行末端聚合的构件，从而形成了丝。众所周知，这些丝与细胞分裂有关，但是仅荧光显微镜尚未揭示足够的细节来彻底理解确切的机制。我们建议使用相关的荧光和X射线成像来确定酵母中每个隔s的位置，即酿酒酵母。