CRYSTALLOGRAPHIC ANALYSIS OF A BACTERIAL MEMBRANE PROTEASE

细菌膜蛋白酶的晶体分析

• 批准号: 8169303
• 负责人: YA HA
• 金额: $ 1.4万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169303
• 关键词: Bacteria; Computer Retrieval of Information on Scientific Projects Database; Crystallization; Funding; Grant; Image; Institution; Integral Membrane Protein; Membrane; Peptide Hydrolases; Process; Research; Research Personnel; Resolution; Resources; Source; Testing; Thick; United States National Institutes of Health; base; beamline; improved; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The objective of the experiment is to screen crystals of type IV prepilin peptidase (TFPP), an integral membrane protein responsible for the cleavage of type IV prepilins in bacteria. TFPP has 8 transmembrane helices. It generated only small-size crystals (<10¿m thickness) and showed low-resolution diffraction at NSLS X29. We wanted to test these crystals at a higher intensity beamline equipped with a microdiffractometer. We were able to take full advantage of the features of 24-ID-E beamline and have obtained some diffraction images with improved resolution (although still insufficient to allow structural determination). Based on the information we obtained from the trip, we are in the process of making further improvement of crystallization conditions.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以出现在其他 CRISP 条目中 列出的机构是。 对于中心来说，它不一定是研究者的机构。 该实验的目的是筛选 IV 型前菌毛蛋白肽酶 (TFPP) 的晶体，TFPP 是一种负责裂解细菌中 IV 型前菌毛蛋白的整合膜蛋白，具有 8 个跨膜螺旋，它仅产生小尺寸晶体（<10¿ m 厚度）并在 NSLS X29 上显示出低分辨率衍射，我们希望在配备微衍射仪的更高强度光束线上测试这些晶体。我们能够充分利用 24-ID-E 光束线的特征，并获得了一些分辨率有所提高的衍射图像（尽管仍不足以进行结构确定），根据我们从这次旅行中获得的信息，我们正在进行中。进一步改进结晶条件。