PNPASE COMPLEX

PNP酶复合物

• 批准号: 8168586
• 负责人: Wen-Hwa Lee
• 金额: $ 1.08万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-15 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168586
• 关键词: Complex; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Human Genome; In Vitro; Institution; Knowledge; Mitochondria; Mitochondrial RNA; Monitor; Proteins; RNA Degradation; Recombinants; Research; Research Personnel; Resources; Role; Source; Testing; United States National Institutes of Health; Work; degradosome; mitochondrial dysfunction

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. There is a readily recognizable PNPase-like molecule in the human genome, which localizes to mitochondria, and its deficiency leads to mitochondrial dysfunction. Importantly, we have found a direct interaction between recombinant SUV3 and PNPase in vitro. We plan to further examine their interaction in detail to test whether SUV3 works in concert with PNPase in monitoring mitochondrial RNA degradation. Are there other proteins in the degradosome together with SUV3/PNPase? If yes, what are their roles in RNA degradation? Isolating and identifying the SUV3/PNPase complex from mitochondria are critical steps to advance our current knowledge of the mammalian mitochondrial degradosome.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 人类基因组中有一种易于识别的 PNPase 样分子，其定位于线粒体，其缺陷会导致线粒体功能障碍。重要的是，我们在体外发现了重组 SUV3 和 PNPase 之间的直接相互作用。我们计划进一步详细研究它们的相互作用，以测试 SUV3 是否与 PNPase 协同监测线粒体 RNA 降解。降解体中是否还有其他蛋白质与 SUV3/PNPase 一起？如果是，它们在 RNA 降解中起什么作用？从线粒体中分离和鉴定 SUV3/PNPase 复合物是推进我们目前对哺乳动物线粒体降解体了解的关键步骤。