STRUCTURAL STUDIES OF THE PHOTOSYNTHETIC REACTION CENTER FROM R SPHAEROIDES

R SPHAEROIDES光合反应中心的结构研究

• 批准号: 8170338
• 负责人: MELVIN Y OKAMURA
• 金额: $ 0.03万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170338
• 关键词: Affect; Bacteriochlorophylls; Binding; Charge; Chemicals; Complex; Computer Retrieval of Information on Scientific Projects Database; Cytochromes c2; Electron Transport; Event; Fibrin fragment D; Funding; Grant; Harvest; Institution; Integral Membrane Protein; Light; Photosynthetic Reaction Centers; Pigments; Proteins; Protons; Quinones; Reaction; Research; Research Personnel; Resources; Source; Structure; United States National Institutes of Health; Water; Work; metal complex; mutant

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Reaction Center (RC), an integral membrane protein, undergoes light induced electron transfer reactions which are the primary steps in converting light into chemical energy. Upon excitation by light, electron transfer occurs from the excited state of a bacteriochlorophyll dimer (D) through intermediate acceptor molecules to two quinone molecules, QA and QB. The RC complexes with another pigment-containing integral membrane protein, the light-harvesting complex (LH1) which gathers and transfers light energy to the RC resulting in photo-oxidized D. Subsequently, a 14-kilodalton water soluble-cytochrome c2 transiently binds to the RC and re-reduces D. Our ongoing work at SSRL will focus on three structure-function aspects of the RC: 1). Determination of the structures of RC-metal complexes and mutants that affect proton and electron transfer events in the RC. 2). Determination of the structures of intermediate states involved in the charge separation. 3). Determination of the structure of the RC complexed with partner proteins: a). RC and light harvesting protein (LH1), and b). Mutants that affect the binding and electron transfer between RC with cyt c2.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 反应中心（RC）是一种完整的膜蛋白，经历光诱导的电子转移反应，这是将光转化为化学能的主要步骤。在光激发下，电子从细菌叶绿素二聚体 (D) 的激发态通过中间受体分子转移到两个醌分子 QA 和 QB。 RC 与另一种含有色素的整合膜蛋白（光捕获复合物 (LH1)）形成复合物，该复合物收集光能并将其转移到 RC，从而产生光氧化 D。随后，14 千道尔顿的水溶性细胞色素 c2 瞬时结合到RC 并重新减少 D。我们在 SSRL 正在进行的工作将重点关注 RC 的三个结构功能方面：1）。确定影响 RC 中质子和电子转移事件的 RC-金属配合物和突变体的结构。 2）。确定电荷分离涉及的中间态结构。 3）。确定与伙伴蛋白复合的 RC 的结构：a)。 RC 和光捕获蛋白 (LH1)，以及 b)。影响 RC 与细胞色素 c2 之间的结合和电子转移的突变体。