PROTEOMICS

蛋白质组学

• 批准号: 8180951
• 负责人: Dean P Edwards
• 金额: $ 16.63万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-17 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8180951
• 关键词: Acetylation; Affinity; Affinity Chromatography; Albumins; Aliquot; Amino Acid Sequence; Amino Acids; Ammonium Sulfate; Antibodies; Antibody Affinity; Antibody Formation; Antigens; Apoptosis; Applications Grants; Area; Arts; Award; Bacteria; Baculoviruses; Binding; Binding Proteins; Binding Sites; Biological Assay; Biological Markers; Biometry; Bioreactors; Biotin; Biotinylation; Blood capillaries; Budgets; Buffers; C-terminal; CD8B1 gene; Cancer Biology; Cancer Center; Cancer Center Support Grant; Cattle; Cell Culture Techniques; Cell Extracts; Cell Line; Cells; Chemistry; Chimeric Proteins; Chromatography; Color; Complement component C1s; Complementary DNA; Complex; Computer software; Consult; Consultations; Coomassie blue; Coupled; Coupling; Culture Media; Custom; Cyclization; Cytolysis; Data; Data Analyses; Data Reporting; Data Set; Databases; Denmark; Detection; Devices; Diagnosis; Diamond; Dimensions; Dissociation; Dyes; Enzyme-Linked Immunosorbent Assay; Epitopes; Equipment; Equipment and supply inventories; Eye; Faculty; Fiber; Fingerprint; Fluorescence; Fractionation; Gel; Genes; Genomics; Glycoproteins; Goals; Gold; Grant; Growth Factor; Height; High Pressure Liquid Chromatography; Human Resources; Hybridomas; Hybrids; Immune response; Immunoblotting; Immunoglobulin G; Immunohistochemistry; Immunoprecipitation; In Vitro; Inbred BALB C Mice; Infection; Injection of therapeutic agent; Insecta; Institution; Investments; Ion-Exchange Chromatography Procedure; Ions; Label; Laboratories; Laboratory culture; Lasers; Ligands; Lipids; Liquid substance; Logistics; Malignant Neoplasms; Maps; Marker Discovery; Mass Spectrum Analysis; Measurement; Mediating; Medicine; Membrane; Metabolic Marker; Methods; Methylation; Mitogen-Activated Protein Kinases; Modeling; Modification; Molecular; Monoclonal Antibodies; Multiple Myeloma; Mus; N-terminal; Nature; Nitrogen; Noise; Nuclear Extract; Nucleopolyhedrovirus; Organization administrative structures; Oryctolagus cuniculus; Pathway interactions; Patients; Peptide Hydrolases; Peptide Sequence Determination; Peptide Synthesis; Peptide antibodies; Peptide/MHC Complex; Peptides; Phase; Phosphoproteins; Phosphorylation; Phosphorylation Site; Plaque Assay; Plasma; Plasmids; Post-Translational Modification Site; Post-Translational Protein Processing; Precipitation; Preparation; Prevention; Procedures; Process; Production; Protein Analysis; Protein Array; Protein Chemistry; Protein Databases; Protein Sequence Analysis; Proteins; Proteome; Proteomics; Proteomics Shared Resource; Prowl; Quality Control; RNA Splicing; Recombinant Proteins; Recombinants; Research; Research Personnel; Resolution; Resource Sharing; Resources; Robot; Running; Sampling; Scanning; Screening procedure; Secondary to; Sepharose; Serum; Serum Proteins; Services; Set protein; Shuttle Vectors; Signal Pathway; Signal Transduction; Silver Staining; Site; Slide; Sodium Dodecyl Sulfate-PAGE; Solutions; Source; Specificity; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spleen; Spottings; Stable Isotope Labeling; Staining method; Stains; Streptavidin; Supervision; Suspension Culture; System; T-Cell Receptor; T-Lymphocyte; Techniques; Technology; Testing; Time; Tissues; Training; Translational Research; Translations; Trypsin; Tube; Two-Dimensional Gel Electrophoresis; UV sensitive; Universities; Validation; Vendor; Viral; Wages; Writing; amidation; base; cancer therapy; capillary; college; cooking; cost effective; cyanine dye 5; cytokine; data acquisition; detector; experience; gel electrophoresis; genetic regulatory protein; homologous recombination; immunocytochemistry; informatics shared resource; instrument; instrumentation; interest; mass spectrometer; meetings; monoclonal antibody production; monomer; mouse model; myristoylation; nano; nano-electrospray; new technology; novel; polyvinylidene fluoride; pre-clinical; pressure; protein complex; protein expression; protein profiling; recombinant virus; reversed phase chromatography; sample collection; tool; tumor; two-dimensional; validation studies; vector; viral DNA

The goal of this shared resource is to provide investigators with cost effective state-of-the-art instrumentation, and specialized expertise for analysis of proteomes with the goal of identifying novel protein biomarkers with applications for prevention, diagnosis and treatment of cancer. Because of the extremely diverse nature of proteins through post-translation modifications (PTMs), secretion, and RNA splicing coupled with their wide dynamic range of expression, no single platform exists for differential analysis of global protein expression. This Shared Resource therefore combines and integrates the instrumentation and expertise of several different faculty co-directors under a single organizational unit directed by Dr. Dean P. Edwards. Through a combined investment by the Institution and the Dan L. Duncan Cancer Center of $1.7M in equipment, start-up salary for new staff and operational support, the Proteomics Shared Resource (PSR) has expanded its technological capabilities and services and has grown tremendously to become one of the heaviest utilized Resources of the Cancer Center. The PSR is currently staffed by four co-directors (Drs Jun Qin, Richard Cook, Shixia Huang, Eastwood Leung) and six highly trained technical staff. New technologies and services developed since the CCSG submission in 2006 include non-mass spectrometry-based protein profiling platforms such as the Luminex fluorescence bead system, two dimensional liquid and gel electrophoresis and antibody/peptide arrays; mass spectrometry identification and PTM analysis of endogenous protein complexes isolated from cells, quantitative mass spectrometry protein profiling by SILAC (stable isotope labeling with heavy amino acids in cells) and enhanced throughput production of monoclonal antibodies (MAbs). These new technologies and services, together with the previously established technologies of protein chemistry, MALDI-TOF identification and PTM analysis of proteins, baculovirus expression of recombinant proteins and conventional hybridoma/MAb production, have created a highly comprehensive Proteomic Shared Resource capable of facilitating both protein biomarker discovery research and translational validation studies through custom antibody production. We will continue to expand and meet the needs of the Cancer Center through shared instrument grant applications and by providing expertise and new leading edge proteomic technologies as the field rapidly evolves.

这一共享资源的目标是为研究人员提供具有成本效益的最先进仪器和蛋白质组分析的专业知识，目的是识别新型蛋白质生物标志物，用于预防、诊断和治疗癌症。由于蛋白质通过翻译后修饰 (PTM)、分泌和 RNA 剪接而具有极其多样化的性质，再加上其广泛的动态表达范围，因此不存在用于全局蛋白质表达差异分析的单一平台。因此，该共享资源将多个不同教职联合主任的仪器和专业知识结合并整合到由 Dean P. Edwards 博士领导的单一组织单位下。通过该机构和 Dan L. Duncan 癌症中心 170 万美元的设备、新员工起薪和运营支持的联合投资，蛋白质组学共享资源 (PSR) 扩大了其技术能力和服务，并取得了巨大发展成为癌症中心利用最频繁的资源之一。 PSR 目前由四名联合主任（秦军博士、Richard Cook、Shixia Huang、Eastwood Leung）和六名训练有素的技术人员组成。自 2006 年 CCSG 提交以来开发的新技术和服务包括基于非质谱的蛋白质分析平台，例如 Luminex 荧光珠系统、二维液体和凝胶电泳以及抗体/肽阵列；对从细胞中分离的内源蛋白质复合物进行质谱鉴定和 PTM 分析，通过 SILAC（细胞中重氨基酸的稳定同位素标记）进行定量质谱蛋白质分析，并提高 单克隆抗体 (MAb)。这些新技术和服务，与先前建立的蛋白质化学、MALDI-TOF 鉴定和蛋白质 PTM 分析、重组蛋白质的杆状病毒表达和常规杂交瘤/单克隆抗体生产技术一起，创建了高度综合的蛋白质组学共享资源，能够促进通过定制抗体生产进行蛋白质生物标志物发现研究和转化验证研究。随着该领域的快速发展，我们将通过共享仪器拨款申请以及提供专业知识和新的前沿蛋白质组技术，继续扩大和满足癌症中心的需求。