CORE--TISSUE CULTURE AND MONOCLONAL ANTIBODY

核心——组织培养和单克隆抗体

• 批准号: 6664429
• 负责人: Dean P Edwards
• 金额: $ 25.04万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-06 至 2003-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6664429
• 关键词: Baculoviridae; antitumor antibody; biomedical facility; hybridomas; monoclonal antibody; tissue /cell culture

Description: (Applicant's Description) The Tissue Culture/Monoclonal Antibody Core (TC/MB) has served for the past 12 years as an efficient resource for UCCC investigators to access specialized cell culture technologies and produce monoclonal antibodies (MAbs). Cell fusion and construction of MAb secreting hybridoma cell lines has been offered since the founding of the facility and continues to be a major function. Production of MAbs from large scale growth of hybridomas in conventional spinner vessels and in hollow-fiber bioreactors was established more recently and this component of hybridoma services has grown by approximately 300% over the past four years. Construction of baculovirus vectors and expression of recombinant proteins in insect cells, established as a service at the last review, has also grown significantly (200-300%) to become a major function of the Core. The TC/MB Core has developed and maintains a repository of more than 150 cancer cell lines in liquid nitrogen and provides UCCC investigators with seed cultures for experiments or cell pellets from large volume cultures as a source for isolation of macromolecules or other cellular factors. In 1997, an On-Site Media Supply Center was established that provides commercially prepared liquid growth medium, supplements and serum at substantially reduced prices and has saved UCCC investigators over $180,000. In addition to technical services, the TC/MB core provides consultation/training and quality control procedures. One or more of the TC/MB Core services has been used by 117 UCCC investigators since the last review of the TC/MB. The users are from 9 of 10 Programs and more than 95% hold peer-reviewed extramural research grants. The custom technological services have provided support for more than 180 publications of UCCC investigators over the last 4 years. The future goal of the TC/MB Core is to continue to provide existing quality services and expand the capacity of custom baculovirus services and large scale growth of hybridomas that have increased the most. To expand the capacity of these services, a small annual increase of 5.5% in Core grant budget is requested so that the technical staff can increase from 3 to 4 individuals. Additionally, new equipment is requested in the second year.

描述：（申请人的描述）组织培养/单克隆 过去 12 年来，Antibody Core (TC/MB) 一直作为一种高效的 供 UCCC 研究人员获取专门细胞培养物的资源 技术并生产单克隆抗体（MAb）。细胞融合和 分泌单克隆抗体的杂交瘤细胞系的构建自 该设施的建立并仍然是一项主要职能。生产 来自传统旋转容器中大规模生长的杂交瘤的单克隆抗体 最近建立了中空纤维生物反应器，该组件 杂交瘤服务的数量在过去四年中增长了约 300% 年。杆状病毒载体的构建及重组表达 昆虫细胞中的蛋白质在上次审查中被确立为一项服务， 也显着增长（200-300%），成为核心的主要功能。 TC/MB 核心开发并维护了超过 150 个的存储库 液氮中的癌细胞系，并为 UCCC 研究人员提供种子 用于实验的培养物或来自大体积培养物的细胞沉淀物作为 分离大分子或其他细胞因子的来源。 1997 年，一个 成立现场媒体供应中心，提供商业化媒体 制备的液体生长培养基、补充剂和血清大幅降低 价格，并为 UCCC 调查人员节省了超过 180,000 美元。此外 技术服务，TC/MB核心提供咨询/培训和质量 控制程序。一项或多项 TC/MB 核心服务已被使用 自上次 TC/MB 审查以来有 117 名 UCCC 调查员。用户来自 10 个项目中的 9 个项目以及超过 95% 的项目拥有经过同行评审的校外研究 补助金。定制技术服务已为多家企业提供支持 UCCC 研究人员在过去 4 年发表的 180 篇出版物。未来的目标 TC/MB 核心的目标是继续提供现有的优质服务和 扩大定制杆状病毒服务的能力和大规模增长 增加最多的杂交瘤。为了扩大这些设施的容量 服务，要求核心拨款预算每年小幅增加 5.5%，以便 技术人员可以从3人增加到4人。此外， 第二年需要新设备。