Epidemiological Research on Autism in Jamaica - Phase II

牙买加自闭症流行病学研究 - 第二阶段

基本信息

批准号：
8411562
负责人：
MOHAMMAD HOSSEIN RAHBAR
金额：
$ 60.74万
依托单位：
UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-09-12 至 2018-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8411562
关键词：
2 year old 3 year old African Age American Area Arsenic Autistic Disorder Behavior Disorders Birth Birth Order Blood capillaries CYP1A1 gene Cadmium Centers for Disease Control and Prevention (U.S.)Chemicals Chemistry Child Clinic Cohort Studies Collaborations Community Health Complex Consumption DNA Data Data Analyses Data Collection Decision Making Developed Countries Development Dietary Questionnaires Economic Burden Educational workshop Enrollment Environment Environmental Exposure Environmental Pollution Environmental Risk Factor Epidemiologic Studies Epidemiology Ethical Issues Etiology Exposure to Family Family Sizes Family history of Fishes Frequencies Funding GSTM1 gene GSTP1 gene GSTT1 gene Gas Chromatography Genes Genetic Genetic Polymorphism Genotype Glutathione S-Transferase Goals Gonadal Steroid Hormones Grant Health Sciences Human Genetics Impairment Intellectual functioning disability Interview Island Jamaica Japanese Population Knowledge Lead Manganese Manuscripts Matched Case-Control Study Measures Medical Research Mercury Metabolism Michigan National Institute of Child Health and Human Development Neurodevelopmental Disorder Newborn Infant Occupations Parents Perinatal Exposure Pharmaceutical Preparations Phase Play Polychlorinated Biphenyls Polymorphism Analysis Population Population Heterogeneity Pregnancy Pregnant Women Prevalence Public Health Public Health Schools Questionnaires Recording of previous events Research Role Saliva Sampling Schools Seafood Serum Smoking Social Interaction Societies Socioeconomic Status Source Statistical Methods Stereotyped Behavior Stereotyping Testing Texas Toxicology Training Transferase Gene Umbilical Cord Blood Universities West Indies aged autism spectrum disorder capillary case control cohort developmental disease drinking early childhood environmental chemical environmental chemical exposure epidemiologic data gene environment interaction in utero indexing infancy neurotoxicity organochlorine pesticide population based prenatal psychologic public health relevance sex social communication steroid metabolism

项目摘要

DESCRIPTION (provided by applicant): Autism Spectrum Disorders (ASDs) are complex lifelong neurodevelopmental and behavioral disorders manifesting in infancy or early childhood, characterized by impairments in social interaction and communication, and by repetitive, stereotyped behavior. Although the causes of ASD are unknown, the evidence suggests that the origins are likely the result of gene- environment interaction occurring in utero or very early in infancy. The long term goal of this project is to expand our capacity for conducting large scale population-based ASD studies in Jamaica. During the past 2-3 years, with funding from NICHD/FIC (R21 HD057808; PI: Rahbar) our research team at the University of Texas Health Science Center at Houston (UTHealth) has collaborated with Dr. Maureen Samms-Vaughan and her colleagues at the University of the West Indies (UWI) in Jamaica to investigate the role of the glutathione-S-transferase (GST) genes and five heavy metals (lead, mercury, arsenic, cadmium, manganese) in relation to ASD. In the proposed project, we will assess the levels of polychlorinated biphenyls (PCBs) and organochlorine (OC) pesticides in the cord-blood isolated from 144 newborns enrolled in the 2011 Jamaican birth cohort to test the hypothesis that children in Jamaica are exposed in utero to these chemicals and identify factors associated with such exposures. In addition, we will conduct an age- and sex-matched case-control study (240 matched pairs) to investigate whether environmental exposures to PCBs and OC pesticides play a role in ASD. Furthermore, we will assess the role of select polymorphisms in GST genes (GSTM1, GSTT1, GSTP1), and their potential interactions with these OCs in relation to ASD. For both cases and controls we will administer a questionnaire to assess the demographic and socioeconomic position, occupation, smoking and drinking status of the parents. In addition, information about family history of developmental disorders, family size, birth order of the index child and whether the child is taking any medications will be collected. Moreover, a dietary questionnaire aimed at capturing possible exposure to environmental contaminants such as PCBs and OC pesticides will be administered. At the end of interview, 1-2 mLs of saliva and about 4-5 mLs of serum will be collected from the case and control children. The DNA isolation and analysis of polymorphisms will be conducted at the Center for Human Genetics at UT School of Public Health. Serum samples will be analyzed for 92 of the 209 PCBs congeners and 18 OC pesticides using capillary column gas chromatography by a CDC-certified lab at the Division of Chemistry and Toxicology at Michigan Department of Community Health in Lansing, Michigan.

描述（由申请人提供）：自闭症谱系障碍（ASD）是复杂的终身神经发育和行为障碍，在婴儿期或幼儿期表现出来，其特征是社会互动和交流的障碍，以及重复性的，刻板的行为。尽管ASD的原因尚不清楚，但证据表明，起源可能是子宫内基因环境相互作用的结果，或者是婴儿期很早就发生的。该项目的长期目标是扩大我们在牙买加进行大规模的基于人群的ASD研究的能力。在过去的2 - 3年中，我们在休斯敦德克萨斯大学健康科学中心（UTHEALTH）的研究团队获得了NICHD/FIC（R21 HD057808; PI：RAHBAR）的资助，与Maureen Samms-Vaughan和她的同事在West Indies（Uwi-sica）中与Maureen Samms-Vaughan和她的同事合作，该作用是jamaia in jamaica in jama in the jamaia in the jama in the jama，（GST）与ASD相关的基因和五种重金属（铅，汞，砷，镉，锰）。在拟议的项目中，我们将评估与从2011年牙买加出生队列中招募的144名新生儿分离的多氯联苯（PCB）（PCB）和有机氯（OC）农药的水平，以测试牙买加的儿童在子宫内对这些化学物质和识别因子的儿童曝光。此外，我们还将进行年龄和性别匹配的病例对照研究（240对匹配），以研究环境对PCB和OC农药的暴露是否在ASD中起作用。此外，我们将评估选择多态性在GST基因（GSTM1，GSTT1，GSTP1）中的作用，以及它们与ASD相关的OCS的潜在相互作用。对于案例和控制，我们将管理问卷调查，以评估父母的人口和社会经济地位，职业，吸烟和饮酒状态。此外，有关发育障碍家族史，家庭规模，指数儿童的出生顺序以及孩子是否服用任何药物的信息。此外，旨在捕获可能接触到环境污染物（例如PCB和OC农药）的饮食问卷。在采访结束时，将从病例和对照儿童中收集1-2毫升的唾液和约4-5毫升的血清。多态性的DNA隔离和分析将在UT公共卫生学院的人类遗传学中心进行。在密歇根州兰辛市密歇根州社区健康部的化学和毒理学部，使用毛细管柱气体色谱法分析了209个PCB同类物中的92例血清样品和18种OC农药。