COBRE: U OF KANSAS MEDICAL CTR: MOLECULAR BIOLOGY CORE

COBRE：堪萨斯大学医学 CTR：分子生物学核心

• 批准号: 8167659
• 负责人: Yu-Jui Yvonne Wan
• 金额: $ 5.64万
• 依托单位: UNIVERSITY OF KANSAS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8167659
• 关键词: Antibodies; Arts; Bacteria; Biological Assay; Centers of Research Excellence; Computer Retrieval of Information on Scientific Projects Database; DNA Sequence; Databases; Densitometry; Equipment; Equipment and supply inventories; Funding; Genetic; Grant; Image; Incubators; Institution; Kansas; Liquid substance; Medical; Molecular Biology; Mouse Cell Line; Plasmids; Polymerase Chain Reaction; Reagent; Research; Research Personnel; Resources; Scientist; Source; System; Time; Tissues; Training; United States National Institutes of Health; Work; cost effective; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Molecular Biology Core will provide all COBRE scientists with technical support, state-of-the-art equipment, and essential molecular biology reagents, so that experiments that require a molecular biology approach can be carried out in a cost-effective way. The facilities provided and maintained by the Core include an ABI Prism 3100 Genetic Analyzer for DNA sequencing, two real-time PCR machines, a Biomek FX Assay Work Station (liquid dispenser), five thermocyclers for polymerase chain reaction, and other commonly used molecular biology equipment, such as an imaging system with the capability for densitometry analysis, bacteria incubators, and ultracentrifuges. The Core is responsible for maintaining and training researchers to use these facilities. In addition, the Core maintains inventory databases for probes, primers, antibodies, cell lines, mouse tissues, and plasmids, which can be shared by COBRE investigators.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 分子生物学核心将为所有 COBRE 科学家提供技术支持、最先进的设备和必要的分子生物学试剂，以便能够以经济有效的方式进行需要分子生物学方法的实验。核心提供和维护的设施包括用于 DNA 测序的 ABI Prism 3100 基因分析仪、两台实时 PCR 机、一台 Biomek FX 分析工作站（液体分配器）、五台用于聚合酶链式反应的热循环仪以及其他常用的分子生物学设备，例如具有光密度分析能力的成像系统、细菌培养箱和超速离心机。核心负责维护和培训研究人员使用这些设施。此外，Core 还维护探针、引物、抗体、细胞系、小鼠组织和质粒的库存数据库，可供 COBRE 研究人员共享。