ENHANCEMENT OF DENGUE VIRUS TRANSMISSION DUE TO SALIVARY PROTEINS OF ITS VECTOR

由于其载体的唾液蛋白而增强登革热病毒的传播

• 批准号: 8167889
• 负责人: Christopher Mores
• 金额: $ 7.38万
• 依托单位: LOUISIANA STATE UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8167889
• 关键词: Aedes; Affect; Antibodies; Arbovirus Infections; Binding; Bite; Complex; Computer Retrieval of Information on Scientific Projects Database; Culicidae; Dengue; Dengue Virus; Ecology; Funding; Grant; Immune response; Infection; Institution; Life; Proteins; Research; Research Personnel; Resources; Salivary Glands; Salivary Proteins; Serotyping; Source; United States National Institutes of Health; base; protein expression; tool; transmission process; vector

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Section 1. Dengue-induced differential protein expression in salivary gland extracts of Aedes aegypti mosquitoes Based on the observed differences in protein expression between infected and uninfected mosquito bodies, we decided to evaluate if this phenomenon was also observed in their salivary glands. Infection of salivary glands by dengue virus is a required step in dengue fever transmission. Section 2. Interaction of Dengue virus with salivary gland proteins Recently, several salivary gland proteins from Aedes mosquitoes were found to bind to dengue virus (Cao-Lormeau et al. 2009). However, it was not clear whether these proteins were structural or secreted. We plan to study salivary gland proteins that are able to bind any of the four serotypes of dengue virus. Section 3. Humoral immune response against Ae. aegypti SGE Salivary gland proteins have shown to be a very useful tool for evaluating mosquito bite exposure. We wanted to evaluate the anti  Ae. aegypti SGE antibody level in people living in dengue endemic regions. Section 4. Transmission dynamics of complex dengue virus ecologies Globally, dengue virus is the most significant arboviral infection, affecting millions of people throughout the tropics. We are investigating the transmission dynamics of dengue virus in the context of multiple, co-circulating serotypes and strains.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 第1节. 登革热诱导的埃及伊蚊唾液腺提取物中差异蛋白表达 根据观察到的受感染和未受感染蚊体之间蛋白质表达的差异，我们决定评估这种现象是否也在它们的唾液腺中观察到。登革热病毒感染唾液腺是登革热传播的必要步骤。 第 2 节. 登革热病毒与唾液腺蛋白的相互作用 最近，发现伊蚊的几种唾液腺蛋白与登革热病毒结合（Cao-Lormeau 等，2009）。然而，尚不清楚这些蛋白质是结构蛋白还是分泌蛋白。我们计划研究能够结合登革热病毒四种血清型中任何一种的唾液腺蛋白。 第 3 节：针对 Ae 的体液免疫反应。埃及伊蚊SGE 唾液腺蛋白已被证明是评估蚊虫叮咬暴露情况的非常有用的工具。我们想要评估抗AE。居住在登革热流行地区的人群中埃及伊蚊 SGE 抗体水平。 第 4 节 复杂登革热病毒生态的传播动力学 在全球范围内，登革热病毒是最重要的虫媒病毒感染，影响着整个热带地区的数百万人。我们正在研究登革热病毒在多种共循环血清型和毒株背景下的传播动态。