CF LUNG MICROBIOME IN ADULTS

成人 CF 肺微生物组

• 批准号: 8167474
• 负责人: George A. O'Toole
• 金额: $ 7.59万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8167474
• 关键词: Aftercare; Antibiotic Therapy; Bacterial DNA; Bacterial Infections; Cessation of life; Chronic; Collaborations; Computer Retrieval of Information on Scientific Projects Database; Cystic Fibrosis; Disease Outcome; Funding; Goals; Grant; Infection; Institution; Lead; Lung; Methodology; Patients; Population; Pseudomonas aeruginosa; Research; Research Personnel; Resources; Respiratory Failure; Respiratory physiology; Source; Sputum; Staphylococcus aureus; Treatment Protocols; United States National Institutes of Health; cystic fibrosis patients; medical schools; microbial; microbiome; pathogen

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our overall goal is to define the microbial populations found in the cystic fibrosis (CF) lung using "deep sequencing" methodologies. The chronic bacterial infections associated with the CF lung contribute to the long-term damage to the lungs of these patients, resulting in respiratory failure. While Pseudomonas aeruginosa and Staphylococcus aureus are the primary pathogens in the CF lung, there is evidence emerging that additional pathogens can contribute to disease outcome, and furthermore, microbial populations may shift upon exacerbation of infection and subsequent treatment of the exacerbations. These exacerbations lead to progress loss of lung function, and eventually to the death of CF patients due to respiratory failure. Detailed studies of shifts in microbial populations over the course of stable infection, exacerbations and post-treatment regimens have not been assessed. Thus, our central hypothesis is that important bacterial pathogens responsible for causing reduction of lung function in CF patients have not yet been identified. A better understanding of the microbial populations under these varied conditions may help facilitate treatment of these chronic infections in the CF lung. In this study, we aim to analyze the bacterial populations within expectorated sputum from patients with stable infections, as well as patients undergoing exacerbations before and after antibiotic therapy. This study represents a collaboration between Drs. George O'Toole, Bruce Stanton, Deborah Hogan (Dartmouth Medical School) and Dr. Worth Parker and colleagues (DHMC). The 454 sequencing will be performed by Dr. Mitch Sogin at WHOI using bacterial DNA prepared at Dartmouth.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 我们的总体目标是使用“深度测序”方法来定义囊性纤维化 (CF) 肺中发现的微生物种群。 与 CF 肺相关的慢性细菌感染会导致这些患者的肺部长期受损，导致呼吸衰竭。虽然铜绿假单胞菌和金黄色葡萄球菌是 CF 肺中的主要病原体，但有证据表明其他病原体可能导致疾病结果，此外，微生物种群可能会在感染恶化和随后的恶化治疗时发生变化。这些恶化导致肺功能逐渐丧失，并最终导致 CF 患者因呼吸衰竭而死亡。尚未评估稳定感染、恶化和治疗后方案过程中微生物种群变化的详细研究。因此，我们的中心假设是，导致 CF 患者肺功能下降的重要细菌病原体尚未确定。更好地了解这些不同条件下的微生物种群可能有助于促进 CF 肺部慢性感染的治疗。在这项研究中，我们的目的是分析稳定感染患者以及抗生素治疗前后病情加重的患者咳痰中的细菌群。这项研究代表了博士之间的合作。 George O'Toole、Bruce Stanton、Deborah Hogan（达特茅斯医学院）以及 Worth Parker 博士及其同事（DHMC）。 454 测序将由 WHOI 的 Mitch Sogin 博士使用达特茅斯制备的细菌 DNA 进行。