COBRE: UNE MED CTR: CORE A: ADMINISTRATIVE

COBRE：UNE MED CTR：核心 A：行政

• 批准号: 8168355
• 负责人: Shelley D Smith
• 金额: $ 48.67万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168355
• 关键词: Address; Advisory Committees; Animal Model; Blindness; Cells; Centers of Research Excellence; Collaborations; Communication; Computer Retrieval of Information on Scientific Projects Database; Core Facility; Development; Education; Ensure; Esthesia; Evaluation; Faculty; Funding; Gene Expression; Gene Mutation; Genetic; Genome engineering; Goals; Grant; Hearing; Hearing Impaired Persons; Histology; Institution; Intervention; Labyrinth; Maintenance; Mentors; Mitotic; Molecular; Molecular Biology; Natural regeneration; Nebraska; Occupations; Participant; Pathology; Pathway interactions; Phase; Pluripotent Stem Cells; Quality of life; Regenerative Medicine; Research; Research Activity; Research Infrastructure; Research Personnel; Resources; Retina; Sensory; Source; Syndrome; System; United States National Institutes of Health; Vision; cell injury; design; interdisciplinary approach; member; molecular phenotype; mouse genome; mouse model; operation; reconstitution; retinal progenitor cell; stem cell population

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The loss of neurosensory function, particularly the sensations of hearing and vision, has devastating effects on communication, education, occupation, and quality of life. Since neurosensory cells of the inner ear and retina are not replaced after damage or degeneration, such losses are permanent. The purpose of the Nebraska Center for the Molecular Biology of Neurosensory Systems is to characterize the genetic mechanisms controlling the development and maintenance of neurosensory functions and the corresponding pathology associated with relevant gene mutations to identify potential avenues for intervention. We will focus our efforts on promising regenerative medicine strategies such as the reconstitution of damaged cells through the use of pluripotent stem cells or de-differentiation and regeneration of post-mitotic cells in the inner ear and retina. These studies utilize specialized animal models that allow control of gene expression at specific critical points in development, with detailed evaluation of the associated findings at the molecular and phenotypic levels. To facilitate these studies, 3 independent research institutions have joined to build an interactive Center with a multidisciplinary approach designed to address to these well-defined objectives. With a core of 3 senior researchers and an exemplary External Advisory Committee, we have successfully mentored 14 junior faculty members and created a collaborative group united by similar thematic and mechanistic approaches. Our research has been facilitated by 3 scientific cores: a Mouse Genome Engineering core for the development of mouse models, a Microarray core for comprehensive assessment of gene expression levels and determination of possible genetic networks, and a Molecular Phenotyping/Histology core to study the results of experimental alterations. These cores are an important addition to the research infrastructure of all 3 institutions. In the second phase of this COBRE project, we will continue to support 6 junior researchers: 2 are studying the mechanisms of specific syndromes of blindness and deaf/blindness, 1 is concentrating on the development and enrichment of retinal stem cell populations, and 3 are studying the molecular pathways of inner ear differentiation with the goal of facilitating sensory regeneration. As those projects obtain external funding and rotate off Center funding, new projects will be added. The addition of new investigators, along with continued support of research activities and the operation of core facilities, will ensure a growing "critical mass" of collaboration that will sustain the Center, and enable participants to obtain external funding which will ensure its long term viability.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 神经感觉功能的丧失，特别是听觉和视觉的感觉，对沟通、教育、职业和生活质量产生毁灭性影响。由于内耳和视网膜的神经感觉细胞在损伤或退化后不会被替换，因此这种损失是永久性的。内布拉斯加州神经感觉系统分子生物学中心的目的是表征控制神经感觉功能发育和维持的遗传机制以及与相关基因突变相关的相应病理学，以确定潜在的干预途径。我们将集中精力研究有前途的再生医学策略，例如通过使用多能干细胞重建受损细胞或内耳和视网膜有丝分裂后细胞的去分化和再生。这些研究利用专门的动物模型，可以在发育的特定关键点控制基因表达，并在分子和表型水平上详细评估相关发现。为了促进这些研究，三个独立研究机构联合建立了一个互动中心，采用多学科方法来实现这些明确的目标。我们以 3 名高级研究人员为核心，并设有一个堪称典范的外部咨询委员会，成功指导了 14 名初级教员，并创建了一个通过类似的主题和机制方法联合起来的协作小组。我们的研究由三个科学核心推动：用于开发小鼠模型的小鼠基因组工程核心、用于全面评估基因表达水平和确定可能的遗传网络的微阵列核心、以及用于研究结果的分子表型/组织学核心的实验性改变。这些核心是对所有 3 个机构的研究基础设施的重要补充。在这个COBRE项目的第二阶段，我们将继续支持6名初级研究人员：2名正在研究失明和耳聋/失明的特定综合征的机制，1名正在专注于视网膜干细胞群体的发育和富集，3名正在研究研究内耳分化的分子途径，以促进感觉再生。随着这些项目获得外部资金并轮换中心资金，新项目将会增加。新研究人员的加入，以及对研究活动和核心设施运营的持续支持，将确保不断增长的合作“临界质量”，以维持该中心，并使参与者能够获得外部资金，从而确保其长期生存能力。