Behavioral and Physiological Phenotyping

行为和生理表型

基本信息

批准号：
8179838
负责人：
ALEXANDER A BACHMANOV
金额：
$ 12.77万
依托单位：
MONELL CHEMICAL SENSES CENTER
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-07-05 至 2016-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8179838
关键词：
Anesthesia procedures Animal Model Animals Anthropology Applications Grants Area Basic Science Behavior Behavioral Biochemistry Body Weight Brain Carbon Dioxide Chemical Engineering Chemical Models Chemicals Collaborations Consultations Data Data Analyses Development Dissection Ecology Electrophysiology (science)Endocrinology Ensure Equipment Equipment and Supplies Experimental Designs Faculty Feeding behaviors Fiber Flavoring Funding Genetic Genetic Engineering Genetically Engineered Mouse Genotype Goals Grant Histology Human Resources Immunology Individual Inflammation Institutes Instruction Interdisciplinary Study Laboratories Maintenance Maps Measures Metabolic Microbiology Microscopic Microscopy Mission Molecular Biology Monitor Motor Activity National Institute on Deafness and Other Communication Disorders Nerve Neurosciences Operative Surgical Procedures Organic Chemistry Oxygen Pharmacology Phenotype Physiological Physiology Production Protocols documentation Psychophysics Public Health Research Research Personnel Resources Scientist Services Smell Perception System Taste Perception Taste Threshold Techniques Testing Tissues Training Training Support Translations Use Effectiveness Zoology base conditioning design drinking behavior experience hedonic improved instrumentation irritation multidisciplinary nutrition preference prototype repaired research study response

项目摘要

SIGNIFICANCE. Many Monell scientists conduct studies with animals and need to characterize their chemosensory phenotypes. They will benefit from the Phenotyping Core in the following ways. 1) This Core will provide a centralized resource of equipment and supplies that will receive dedicated support and regular maintenance. The Core will also centralize labor-intensive tasks, such as making equipment for preference tests. Studies will be designed and/or conducted by Core personnel with specialized expertise in phenotyping techniques. This will be more efficient than replicating these techniques and personnel in individual laboratories. Regular use and service will ensure that equipment is always operational and available for experimentation. 2) The Core will provide access to equipment and facilities (e.g., LabMaster, surgical facility) that are not practical to maintain in individual laboratories. The Core will offer a range of services and experimental designs that are not available in individual laboratories. 3) Scientists with no experience in animal phenotyping will receive training and will have access to equipment and expertise of the Core. This will facilitate their research and help them to collect preliminary data for grant applications. 4) This Research Core will be an integral component of the Core Center. Interactions between the Phenotyping Core and other Research Cores will facilitate collaborative and interdisciplinary studies that are often not feasible for individual laboratories. For example, the Phenotyping and Histology Cores will be used to characterize genetically engineered mice generated from constructs produced using the Molecular Biology Core. In forward genetics studies, the same animals will be examined using the Phenotyping and Genotyping Cores, and the data obtained will be used for chromosomal mapping studies. We estimate that 9 of the 13 R01 grants in our Research Base will use services provided by this Core at moderate to extensive levels. We also expect that 11 current faculty research groups at Monell will use the services provided by this Core. As a result, the Phenotyping Core will improve the efficiency and quality of animal model studies at the Center, accelerate existing NIDCD-funded projects, and advance translation of the results into benefits for public health.

意义。许多莫内尔科学家对动物进行研究，需要表征它们的化学感应表型。他们将通过以下方式受益于表型核心。 1) 该核心将提供集中的设备和物资资源，并将获得专门的支持和定期维护。该核心还将集中处理劳动密集型任务，例如制造用于偏好测试的设备。研究将由具有表型技术专业知识的核心人员设计和/或进行。这将比在各个实验室复制这些技术和人员更有效率。定期使用和维护将确保设备始终运行并可用于实验。 2) 核心将提供对单个实验室无法维护的设备和设施（例如 LabMaster、手术设施）的访问。该核心将提供一系列服务和实验设计，这是单个实验室所不具备的。 3) 没有动物表型分析经验的科学家将接受培训，并获得核心的设备和专业知识。这将促进他们的研究并帮助他们收集拨款申请的初步数据。 4) 该研究核心将成为核心中心的一个组成部分。表型核心和其他研究核心之间的相互作用将促进协作和跨学科研究，而这对于单个实验室来说通常是不可行的。例如，表型分析和组织学核心将用于表征由使用分子生物学核心产生的构建体产生的基因工程小鼠。在正向遗传学研究中，将使用表型和基因分型核心检查相同的动物，获得的数据将用于染色体作图研究。我们估计，我们研究基地的 13 项 R01 资助中有 9 项将在中等至广泛水平上使用该核心提供的服务。我们还预计莫内尔大学现有的 11 个教职研究小组将使用该核心提供的服务。因此，表型分析核心将提高该中心动物模型研究的效率和质量，加速现有 NIDCD 资助的项目，并推动将结果转化为公共健康效益。