Microscope Imaging Module

显微镜成像模块

基本信息

批准号：
8685380
负责人：
RICHARD H KRAMER
金额：
$ 13.84万
依托单位：
UNIVERSITY OF CALIFORNIA BERKELEY
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8685380
关键词：
Animals Biological Assay Cell physiology Cells Collaborations Collection Communities Cornea Custom Development Engineering Equipment Eye Financial Support Fluorescent Probes Glutamates Goals Grant Hour Image Imaging technology Individual Ion Channel Length Light Lighting Maintenance Measures Microscope Microscopic Microscopy Modification Mus Optics Participant Pattern Photons Photoreceptors Phototoxicity Research Research Personnel Resolution Retina Scientist Sorting - Cell Movement Specific qualifier value Structure System Technical Expertise Techniques Technology Time Tissues Vision Vision research Wages Work adaptive optics austin azobenzene base cost cost effective design in vivo instrument instrumentation interest meetings member millisecond molecular imaging new technology optical imaging prevent response two-photon vision science

项目摘要

Purpose: Microscopic Imaging Module Vision scientists have used optical imaging to assay function at a variety of length scales - from subcellular processes to collections of cell organized into networks. With the advent of new fluorescent probes and imaging technologies, the possibilities of both measuring and manipulating cells and circuit function are more powerful than ever. Two notable breakthroughs in the past few years have revolutionized the ability of vision scientists to manipulate and probe excitable cells in intact tissue. First, the development of two-photon microscopy allows for the stimulation of fluorescent probes deep in tissue with a minimum amount of phototoxicity. Second, ion channels have been engineered to open and close in response to light, allowing for spatially localized stimulation and/or silencing of individual cells with millisecond time resolution. The vision science community at UC Berkeley is unique in that several of its members have been at the forefront of developing and utilizing these and other new optical technologies that are revolutionizing vision research. UC Berkeley is also fortunate in having several advanced microscopy systems on campus. In particular, the Molecular Imaging Center (MIC) in the LSA building has five confocal/2-photon imaging systems, a spinning disk confocal system with a spatial-light-modulator used for optical excitation, and soon-to-be installed PALM microscope for super-resolution imaging, all designated for shared use

用途：显微成像模块视觉科学家使用光学成像来分析各种长度尺度的功能——从亚细胞过程到组织成网络的细胞集合。随着新的荧光探针和成像技术的出现，测量和操纵细胞和电路功能的可能性比以往任何时候都更加强大。过去几年的两项显着突破彻底改变了视觉科学家操纵和探测完整组织中可兴奋细胞的能力。首先，双光子显微镜的发展允许以最小的光毒性刺激组织深处的荧光探针。其次，离子通道被设计为响应光而打开和关闭，从而允许以毫秒时间分辨率对单个细胞进行空间局部刺激和/或沉默。加州大学伯克利分校的视觉科学界是独一无二的，因为它的一些成员一直处于开发和利用这些以及其他正在彻底改变视觉研究的新光学技术的最前沿。加州大学伯克利分校还幸运地在校园内拥有多个先进的显微镜系统。特别是，LSA 大楼的分子成像中心 (MIC) 拥有五个共焦/2 光子成像系统、一个带有用于光学激发的空间光调制器的转盘共焦系统，以及即将安装的 PALM 显微镜用于超分辨率成像，全部指定供共享使用