DNA ANALYSIS CORE FACILITY

DNA 分析核心设施

• 批准号: 8357081
• 负责人: KYLE L JOHNSON
• 金额: $ 16.76万
• 依托单位: UNIVERSITY OF TEXAS EL PASO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357081
• 关键词: Biomedical Research; Blood capillaries; Core Facility; Custom; DNA; DNA Microarray Chip; DNA Sequence; DNA Sequence Analysis; DNA analysis; Data Analyses; Data Collection; Documentation; Equipment; Fees; Floor; Funding; Gel; Genetic; Grant; Human Resources; Laboratories; Mexico; National Center for Research Resources; Principal Investigator; Printing; Process; Protein Microchips; Protocols documentation; RNA; Reaction; Research; Research Infrastructure; Research Personnel; Resources; Reverse Transcription; Scanning; Services; Slide; Source; System; Technical Expertise; Techniques; Texas; Training; United States National Institutes of Health; Update; capillary; cost; design; genome sequencing

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The analysis of DNA and RNA is an essential aspect of cutting-edge biomedical research. In order to facilitate the application of state-of-the-art techniques to research problems relevant to the Texas/Mexico border region, this proposal outlines new initiatives for the DNA Analysis Core Facility. The updated Core combines existing equipment for quantitative reverse-transcription PCR (qRT-PCR), data collection and analysis (UV/VIS spectrophotometers, gel documentation system, and storage PhorphorImager), and custom microarray printing, hybridization and scanning equipment with a newly-acquired high capacity capillary DNA sequencer. The ability to perform high-throughput genome sequencing will augment existing technical strengths in microarray design and processing. Implementation of a sliding fee scale for DNA sequencing and microarray services provided to BBRC researchers, UTEP investigators outside the BBRC, and to regional and national researchers outside of UTEP will allow the DNA Analysis Core Facility to transition to a self-supporting status. The relocation of the Core to new, greatly expanded laboratory space on the 3rd floor of the Bioscience Research Building, together with the addition of new technical expertise, will permit the DNA Analysis Core to keep pace with the rate of demand placed upon it by an increasing number of biomedical researchers. We propose the following Specific Aims: 1. Respond to increasing microarry use by assisting researchers in developing protocols and optimizing reaction conditions for DNA and protein microarrays 2. Develop protocols for DNA sequence analysis using the newly acquired Genetic Analyzer and train appropriate personnel in its use 3. Transform the Core into a self-sustainable facility that provides the region with rapid, top-quality DNA sequencing and microarray services

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 DNA 和 RNA 分析是尖端生物医学研究的一个重要方面。为了促进应用最先进的技术来研究与德克萨斯州/墨西哥边境地区相关的问题，该提案概述了 DNA 分析核心设施的新举措。更新后的 Core 将现有的定量逆转录 PCR (qRT-PCR)、数据收集和分析设备（UV/VIS 分光光度计、凝胶文档系统和存储 PhorphorImager）以及定制微阵列打印、杂交和扫描设备与新的设备相结合。获得高容量毛细管DNA测序仪。进行高通量基因组测序的能力将增强微阵列设计和处理方面的现有技术优势。对向 BBRC 研究人员、BBRC 之外的 UTEP 研究人员以及 UTEP 之外的区域和国家研究人员提供的 DNA 测序和微阵列服务实行浮动收费标准，将使 DNA 分析核心设施过渡到自给自足状态。将核心搬迁到生物科学研究大楼三楼的新的、大大扩展的实验室空间，加上新技术专业知识的增加，将使 DNA 分析核心能够跟上市场对其的需求速度。生物医学研究人员数量不断增加。我们提出以下具体目标： 1. 通过协助研究人员制定 DNA 和蛋白质微阵列方案并优化反应条件，应对微阵列使用的增加 2. 使用新购置的遗传分析仪制定 DNA 序列分析方案，并对适当的人员进行使用培训 3. 将核心转变为自我可持续的设施，为该地区提供快速、优质的 DNA 测序和微阵列服务