PROMOTER CHARACTERIZATION OF THE CART GENE

Cart 基因的启动子特征

• 批准号: 8357398
• 负责人: MICHAEL J KUHAR
• 金额: $ 3.29万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357398
• 关键词: Animals; Behavioral; Brain; Funding; Genes; Grant; Injection of therapeutic agent; National Center for Research Resources; Peptides; Primates; Principal Investigator; Research; Research Infrastructure; Resources; Source; United States National Institutes of Health; Viral; cocaine- and amphetamine-regulated transcript protein; cost; particle; promoter; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Most studies of CART peptides involve injecting the peptide into the brain and studying its effects. Therefore, this complementary project aimed at reducing endogenous CART was initiated. Several siRNAs have been found that, in preliminary experiments, reduce CART peptide levels. Also, injection of viral particles that will express siRNAs long term has been carried out. Useful behavioral studies are being carried out in animals where CART peptide levels have been reduced.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 大多数 CART 肽研究都涉及将肽注射到大脑中并研究其效果。 因此，启动了这个旨在减少内源性 CART 的补充项目。 在初步实验中，已发现几种 siRNA 可以降低 CART 肽水平。此外，已经进行了长期表达 siRNA 的病毒颗粒的注射。有用的行为研究正在动物身上进行，其中 CART 肽水平已降低。