MOTOR EFFECTS DERMAL FIBROBLAST GRAFTS IN GLOBUS PALLIDUS- PARKINSONIAN PRIMATES

苍白球-帕金森病灵长类动物真皮成纤维细胞移植物的运动效应

• 批准号: 8357537
• 负责人: Stella M Papa
• 金额: $ 3.29万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357537
• 关键词: Animals; Basal Ganglia; Behavior; Biopsy; Cultured Cells; Dermal; Dyskinetic syndrome; Fibroblasts; Funding; Globus Pallidus; Grant; Levodopa; Location; Maps; Microelectrodes; Monkeys; Motor; National Center for Research Resources; Neurons; Parkinson Disease; Parkinsonian Disorders; Primates; Principal Investigator; Research; Research Infrastructure; Resources; Rodent Model; Skin; Source; Symptoms; Testing; Transplantation; United States National Institutes of Health; behavior test; cost; improved

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Dermal fibroblasts grafted in the globus pallidus have been shown to modulate the neuronal activity and improve motor behavior in rodent models of Parkinson's disease. In this project, we will study the transplantation of fibroblasts into the globus pallidus of parkinsonian monkeys to evaluate effects on parkinsonian symptoms. We plan to obtain fibroblast from skin biopsy and subsequently culture the cells for grafting. In aim 1, we plan to test the accuracy of location of the graft using microelectrode-guided mapping of basal ganglia regions in a normal monkey. In Aim 2, the fibroblast transplantation will be carried out in MPTP parkinsonian monkeys. In these parkinsonian monkeys, tests of levodopa-induced dyskinesias will be preformed for three months after the transplantation. These animals will be sacrificed at the end of the behavioral testing period. This project has just started with Aim 1.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 移植到苍白球的真皮成纤维细胞已被证明可以调节帕金森病啮齿动物模型的神经元活动并改善运动行为。在这个项目中，我们将研究将成纤维细胞移植到帕金森猴苍白球中，以评估对帕金森症状的影响。我们计划从皮肤活检中获取成纤维细胞，然后培养细胞用于移植。在目标 1 中，我们计划使用正常猴子基底神经节区域的微电极引导绘图来测试移植物定位的准确性。在目标2中，将在MPTP帕金森猴中进行成纤维细胞移植。 在这些帕金森猴中，移植后三个月将进行左旋多巴引起的运动障碍测试。这些动物将在行为测试期结束时被处死。该项目刚刚从目标 1 开始。