VivaCT 40 Scanner

VivaCT 40 扫描仪

• 批准号: 8052441
• 负责人: LAURIE Hollis GLIMCHER
• 金额: $ 39.67万
• 依托单位: HARVARD SCHOOL OF PUBLIC HEALTH
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-15 至 2012-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8052441
• 关键词: Anesthesia procedures; Animal Barrier Facility; Animals; Arthritis; Behavior; Biocompatible Materials; Biomedical Research; Bone remodeling; Boston; Cartilage Matrix; Chondrocytes; Communities; Computers; Contract Services; Data Analyses; Dental Schools; Development; Evaluation; Faculty; Funding; Genetic; Genetically Engineered Mouse; Human; Individual; Isoflurane; Joints; Learning; Malignant Neoplasms; Medical; Medicine; Minor; Modeling; Molecular; Mus; Osteoblasts; Osteoclasts; Osteoporosis; Pathway interactions; Pre-Clinical Model; Process; Public Health; Public Health Schools; Rodent; Site; Skeletal system; Stress; System; Techniques; Time; Validation; X-Ray Computed Tomography; base; bone; bone loss; data acquisition; improved; in vivo; instrument; interest; member; mouse model; preclinical study; public health relevance; skeletal; skeletal disorder

DESCRIPTION (provided by applicant): We are requesting funding for an In Vivo micro-computerized tomography (<CT) system from SCANCO Medical (Viva-CT 40) to be sited in the barrier animal facility at the Harvard School of Public Health (HSPH) for use by the biomedical research community at HSPH and the Harvard School of Dental Medicine (HSDM) in Boston. This system consists of the Viva-CT 40 scanner, two computers for data acquisition and analysis, and an isoflurane based anesthesia system. The In Vivo <CT will be primarily used to evaluate genetically engineered mice in established and developmental models of bone loss and accrual as well as arthritis. Bone and joints are remarkable biomaterials that model and remodel to grow, preserve structural integrity and adapt to stress. This dynamic process is controlled by bone forming osteoblasts, bone resorbing osteoclasts and cartilage matrix secreting chondrocytes. An imbalance between catabolic and anabolic activity within bone and joints contributes to some of the most common public health problems including osteoporosis, arthritis and cancer. Much has been learned about the molecular control of bone remodeling by the analysis of genetically engineered mouse models of these human conditions. Importantly, humans and rodents share the majority of pathways that control osteoblast, osteoclast and chondrocyte behavior. The relatively recent availability of In Vivo <CT machines has made it possible for biologists interested in the skeletal system to follow skeletal parameters in the same mouse over time. This technique could vastly improve the quality of the currently funded studies from the major (Glimcher, Olsen and Baron) and minor (Hotamisligil, Aliprantis, Jones, Lankse, and Li) users included in this proposal who each use mouse genetics to probe the skeletal system. The Viva CT 40 will be incorporated into the existing <CT facility at HSDM where we now routinely perform Ex Vivo <CT. A strong commitment from the administration at HSPH completes this application by assuring a long- term service contract for the instrument as well as support for technical assistance. In summary, the Viva CT 40 will allow us to longitudinally compare bone parameters in the same mouse over time thereby substantially reducing the number of animals needed for robust validation of preclinical studies and facilitating the timely completion of our PHS funded projects. Public Health Relevance: Ten faculty members at HSPH and HSDM who each use mouse genetics to probe the skeletal system are requesting funding for an In Vivo CAT scan system from SCANCO Medical (Viva-CT 40). This instrument allows the evaluation of individual genetically engineered mice in models of bone loss and accrual over time and will provide robust validation of preclinical models of human skeletal diseases such as osteoporosis and arthritis.

描述（由申请人提供）：我们正在向 SCANCO Medical (Viva-CT 40) 申请资助用于安装在哈佛大学公共卫生学院 (HSPH) 屏障动物设施中的体内微型计算机断层扫描 (<CT) 系统）供 HSPH 和波士顿哈佛大学牙科医学院 (HSDM) 的生物医学研究界使用。该系统由 Viva-CT 40 扫描仪、两台用于数据采集和分析的计算机以及基于异氟烷的麻醉系统组成。 In Vivo <CT 将主要用于在已建立和发育的骨质流失、骨质增生以及关节炎模型中评估基因工程小鼠。骨骼和关节是卓越的生物材料，可以建模和重塑以生长、保持结构完整性并适应压力。这一动态过程由骨形成成骨细胞、骨吸收破骨细胞和分泌软骨细胞的软骨基质控制。骨骼和关节内分解代谢和合成代谢活动之间的不平衡会导致一些最常见的公共卫生问题，包括骨质疏松症、关节炎和癌症。通过分析这些人类疾病的基因工程小鼠模型，我们对骨重塑的分子控制有了很多了解。重要的是，人类和啮齿动物共享大多数控制成骨细胞、破骨细胞和软骨细胞行为的途径。最近出现的体内 CT 机器使对骨骼系统感兴趣的生物学家能够随着时间的推移跟踪同一只小鼠的骨骼参数。这项技术可以极大地提高当前资助的研究质量，这些研究来自本提案中包括的主要用户（Glimcher、Olsen 和 Baron）和次要用户（Hotamisligil、Aliprantis、Jones、Lankse 和 Li），他们各自使用小鼠遗传学来探测骨骼系统。 Viva CT 40 将并入 HSDM 现有的 <CT 设施，我们现在在那里例行执行 Ex Vivo <CT。 HSPH 管理部门的坚定承诺通过确保仪器的长期服务合同以及技术援助支持来完成此申请。总之，Viva CT 40 将使我们能够纵向比较同一只小鼠随时间的骨骼参数，从而大大减少临床前研究可靠验证所需的动物数量，并促进我们 PHS 资助项目的及时完成。 公共健康相关性：HSPH 和 HSDM 的 10 名教职员工均使用小鼠遗传学来探测骨骼系统，他们正在向 SCANCO Medical (Viva-CT 40) 申请资助用于体内 CAT 扫描系统。该仪器可以在骨质流失和随时间增加的模型中评估个体基因工程小鼠，并将为骨质疏松症和关节炎等人类骨骼疾病的临床前模型提供强有力的验证。