Towards Viable Nanopore Sequencing by Slowing DNA Translocation

通过减缓 DNA 易位实现可行的纳米孔测序

• 批准号: 8570021
• 负责人: Anna Schibel
• 金额: $ 25万
• 依托单位: ELECTRONIC BIOSCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2015-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8570021
• 关键词: Address; Biochemical; Biological; Characteristics; DNA; DNA Binding; DNA Sequence; Development; Discrimination; Disease; Electronics; Engineering; Enzymes; Escherichia coli; Evaluation; Failure; Genetic; Genome; Goals; Government; Health; Hemolysin; Human; Individual; Lead; Lipids; Measurement; Medicine; Methods; Motor; Nature; Noise; Nucleotides; Performance; Persons; Phase; Polynucleotides; Preparation; Proteins; RNA; Reading; Reagent; Relative (related person); Residual state; Right-On; SS DNA BP; Sampling; Single-Stranded DNA; Site-Directed Mutagenesis; Solutions; Speed; Stretching; Structure; System; Techniques; Technology; Time; base; biological systems; cost; electrical measurement; genome sequencing; improved; innovation; insertion/deletion mutation; instrumentation; melting; nanopore; novel; pathogen; programs; public health relevance; silicon nitride; single molecule; solid state; success; tool

DESCRIPTION (provided by applicant): The aim of this project is to investigate a simple method for slowing down the rate of electrophoretic translocation of DNA through a nanopore. Unique modulations of current across nanopores produced by individual bases during such translocation forms the basis of DNA nanopore sequencing. The nanopore method requires very simple sample preparation with potentially no amplification steps and can achieve long read sequences with inexpensive hardware and biochemical reagents. Hence, nanopore technology holds a great deal of promise in the effort to achieve rapid, low cost sequencing. However, current high bandwidth methods are limited by the inability to accurately distinguish various bases due to the extremely rapid nature of freely translocating DNA. Methods proposed here maintain the simplicity of nanopore sequencing through biological and electronic innovations. Progress made through this proposal will potentially lead to an inexpensive and rapid benchtop DNA sequencing solution that will contribute significantly towards the goal of the $1000 genome.

描述（由申请人提供）：该项目的目的是研究一种简单的方法，以减慢DNA通过纳米孔的电泳易位速率。在此类易位期间，各个碱基产生的纳米孔中电流的独特调制构成了DNA纳米孔测序的基础。纳米孔方法需要非常简单的样品制备，并可能没有扩增步骤，并且可以使用廉价的硬件和生化试剂实现长读序列。因此，Nanopore技术在实现快速，低成本测序的努力方面具有很大的希望。但是，由于自由易位DNA的极快性质，当前的高带宽方法无法准确区分各种碱基。这里提出的方法通过生物学和电子创新保持纳米孔测序的简单性。通过该提案取得的进展可能会导致廉价且快速的台式DNA测序解决方案，这将对1000美元的基因组的目标产生重大贡献。