INBRE: URI: CENTRALIZED RES CORE FACILITY

INBRE：URI：集中资源核心设施

基本信息

批准号：
8167602
负责人：
AFTAB J AHMED
金额：
$ 17.31万
依托单位：
UNIVERSITY OF RHODE ISLAND
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-05-01 至 2011-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8167602
关键词：
Aerosols Apoptosis Biological Assay Biological Markers Biomedical Research Cells Charge Chemicals Collaborations Communities Computer Retrieval of Information on Scientific Projects Database Consultations Core Facility Educational process of instructing Educational workshop Environment Equipment Faculty Fees Frequencies Funding Future G Protein-Coupled Receptor Genes Genes Grant Hand Institution Label Laboratories Manufacturer Name Nucleic Acids Participant Pharmacy facility Postdoctoral Fellow Printing Production Reader Recommendation Research Research Personnel Research Project Grants Research Training Resources Rhode Island Safety Services Source Students System Techniques Technology Time Toxic effect Toxicology Training Training Support United States National Institutes of Health Vision base college cost design detector experience frontier graduate student instrument instrumentation migration novel posters programs small molecule

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The RI-INBRE Centralized Research Core Facility is in its seventh year of providing research and training support to the Network participants and the Rhode Island biomedical research community. During the current grant period more than 57 research groups have used the Facility, with an average quarterly cumulative frequency of 1,200 usages. The Facility users consisted of 32 faculty, 31 postdoctoral fellows, 93 graduate students, and 20 undergraduate students. The Facility staff provided training and consultation to all new users and in order to better utilize various technologies in their research projects. In addition, the staff continued to participate in teaching various techniques in the undergraduate and graduate laboratory courses offered by the College of Pharmacy. These courses are designed to provide both theoretical and hands-on experience to students using the Facility's equipment. Throughout the year, several instructional seminars and training workshops were organized in collaboration with the instrument manufacturers. The topics included: "Discovery and characterization of novel biomarkers using SELDI-TOF technology"; "Xcelligence System a new frontier in label free, real time cell based assays for apoptosis, and GPCR invasion migration"; " QSTAR-MS/MS in a small molecule environment"; " Charged Aerosol Detector (CAD) a novel mass sensitive universal detector for the routine determination of any non-volatile chemical species; and "Profiling chemical toxicity using CDAS safety toxicology platform in the EPA ToxCast program". Based on the recommendations received from the RI-IBRE participants, new equipment acquired this year included the following: Quick Gene 810 nucleic acid isolation system; Polarstar Optima microplate reader' and Cellometer Vision automated cell counter. The SEDI-TOF system has become obsolete because BioRad has discontinued future production and upgrade. The Facility will be looking to replace this system with a new TOF-TOF machine with SELDI capability. A total of 170 posters were printed with non-RI-INBRE researchers paying a modest fee to cover the cost of consumables. The equipment and consultation were continued to be made available to the researchers at no cost. Overall, the usage of the Core Facility instrumentation and its consultative services has continued to increase.

该子项目是利用该技术的众多研究子项目之一资源由 NIH/NCRR 资助的中心拨款提供。子项目和研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金，因此可以在其他 CRISP 条目中表示。列出的机构是对于中心来说，它不一定是研究者的机构。 RI-INBRE 集中研究核心设施为网络参与者和罗德岛生物医学研究界提供研究和培训支持已进入第七个年头。在当前资助期内，超过 57 个研究小组使用了该设施，平均每季度累计使用次数为 1,200 次。该设施的用户包括 32 名教职人员、31 名博士后研究员、93 名研究生和 20 名本科生。设施工作人员为所有新用户提供培训和咨询，以便更好地在他们的研究项目中利用各种技术。此外，工作人员继续参与药学院提供的本科生和研究生实验室课程中的各种技术教学。这些课程旨在为使用该设施设备的学生提供理论和实践经验。全年与仪器制造商合作举办了多次教学研讨会和培训讲习班。主题包括：“利用 SELDI-TOF 技术发现和表征新型生物标志物”； “Xcelligence 系统是无标记、实时细胞凋亡和 GPCR 侵袭迁移检测的新前沿”； “小分子环境中的 QSTAR-MS/MS”； “带电气溶胶检测器 (CAD) 是一种新型质量敏感通用检测器，用于常规测定任何非挥发性化学物质；以及“使用 EPA ToxCast 计划中的 CDAS 安全毒理学平台分析化学毒性”。基于 RI 收到的建议-IBRE参与者，今年购买的新设备包括：Quick Gene 810核酸分离系统；Polarstar Optima酶标仪和Cellometer Vision自动细胞计数仪已成为SEDI-TOF系统。已过时，因为 BioRad 已停止未来的生产和升级，该机构将寻求用具有 SELDI 功能的新型 TOF-TOF 机器取代该系统。非 RI-INBRE 研究人员只需支付少量费用即可印刷 170 张海报。继续向研究人员免费提供设备和咨询。总体而言，核心设施仪器及其咨询服务的使用量持续增加。