In Situ Sampling Tool for Assessing Bioavailability and Toxicity of Sediments

用于评估沉积物生物利用度和毒性的原位采样工具

• 批准号: 8514608
• 负责人: Nancy D Denslow
• 金额: $ 27.13万
• 依托单位: ARIZONA STATE UNIVERSITY-TEMPE CAMPUS
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-20 至 2016-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8514608
• 关键词: Address; Adsorption; Amendment; Analytical Chemistry; Arizona; Behavior; Bioavailable; Biological; Biological Assay; Biological Availability; Body Burden; Carbon; Chemical Structure; Chemicals; Computer-Aided Design; Consumption; DNA Microarray Chip; Data; Data Analyses; Detection; Devices; Dichlorodiphenyl Dichloroethylene; Dieldrin; Effectiveness; Environment; Environmental Pollutants; Equilibrium; Family; Farming environment; Filtration; Fishes; Florida; Funding; Gene Expression; Goals; Hazardous Waste Sites; Health; Home environment; Hour; Human; In Situ; Laboratories; Laboratory Study; Legal patent; Mass Spectrum Analysis; Measurement; Measures; Methods; Microarray Analysis; Minnows; Modeling; Monitor; Organism; Parents; Pathway interactions; Performance; Phase; Procedures; Process; Protocols documentation; Pump; Research Project Grants; Risk; Risk Assessment; Sampling; Series; Signal Recognition Particle; Site; Solid; Stainless Steel; Superfund; Surface; System; Techniques; Technology; Testing; Time; Toxic effect; Triclosan; Tubular formation; Universities; Water; Water Pollutants; Work; adverse outcome; aged; base; bioaccumulation; design; exposed human population; field study; fipronil; high throughput screening; mathematical model; novel; novel strategies; physical property; pollutant; programs; prototype; receptor; remediation; research study; response; simulation; superfund site; tandem mass spectrometry; theories; tool; toxicant; triclocarban; uptake; water flow; water sampling

ABSTRACT This project introduces a novel strategy for bioavailability determination of sediment-borne contaminants featuring a wide range of chemical structures, properties and physical-chemical behaviors. The project addresses the pressing need of Superfund stakeholders to determine in a convenient and reliable fashion both human health risks from contaminated sediments and the effectiveness of implemented remediation strategies. The in situ sampling/bioavailability determination (IS2B) tool is a novel, patent-pending device enabling simultaneous determination of contaminant levels in bulk water and pore water at hitherto unattainably low method detection limits (MDLs). When deployed, one half of the tubular IS2B device is buried in sediment and the other is exposed to bulk water. Integrated multi-channel pumps simultaneously draw bulk water and sediment pore water into the active sampling device and push it at, respectively, high and low desirable flow rates through an array of filters and adsorption media. Water samples either can be stored in the device or expelled into the bulk water at will. Due to unlimited access to pore and bulk water during deployment, the IS2B tool provides ultra-low MDLs for a broad spectrum of contaminants, ranging from fully water-soluble to highly sorptive and hydrophobic. This functionality distinguishes the device from presently available passive sampling strategies. Know-how from three previous SRP projects is being leveraged to test the working hypothesis that the IS2B technology can serve to reliably (i) sample bulk and pore water pollutants for analysis in the sub-ng/L range, (ii) inform on the bioavailability and bioactivity of sediment contaminants, (iii) inform on human exposures and associated health risks from fish consumption, and (iv) track the progress of sediment remediation activities. Laboratory and field studies will be conducted with contaminated sediments from Lake Apopka, home to one of Florida's various Superfund sites. To illustrate the breadth of IS2B applicability, this project concentrates on two traditional and three emerging sediment contaminants (p,p'-DDE, dieldrin versus fipronil, triclosan, triclocarban). Because there may be discrepancies between the bioaccumulation predicted via modeling from sampler data and actual bioaccumulation, bioavailability and bioaccumulation of pollutants will be assessed in lab experiments with freshly spiked and long-term aged contaminated sediments via determination of body burdens in two test organisms: Lumbriculus variegatus and Pimephales promelas. Biological responses to contaminant exposure will also be measured in fish using DNA microarray analysis to evaluate effects that may not be predicted solely based on body burden of parent compounds. Mathematical relationships between pollutant concentrations in bulk water, pore water, worms and fish will be formulated from theory and lab data, and applied to IS2B-derived data to predict risk and track the effectiveness of two remediation approaches, granular activated carbon (GAC) amendment and deep tilling of contaminated sediment.

抽象的 该项目介绍了一种新颖的策略，以确定沉积物 - 元素污染物的生物利用度 具有广泛的化学结构，特性和物理化学行为。项目 解决了超级基金利益相关者以方便和可靠的方式确定的紧迫需求 人类健康风险受到污染的沉积物以及实施补救策略的有效性。 原位采样/生物利用度确定（IS2B）工具是一种新颖的，正在申请专利的设备，可实现 同时测定迄今为止低落的大量水和孔隙水中污染物水平 方法检测极限（MDL）。部署时，管状IS2B设备的一半埋在沉积物中， 另一个暴露在散装水中。集成的多通道泵同时抽水， 沉积物孔水进入活动采样装置，并分别将其推到高和低期的流动 通过一系列过滤器和吸附介质的速率。水样可以存储在设备中或 随意驱逐到散装水中。由于部署期间无限获得毛孔和散装水， IS2B工具为各种污染物提供了超低MDL，从完全水溶液到 高度吸附和疏水。此功能将设备与目前可用的被动区分开 抽样策略。 先前三个SRP项目的专业知识正在利用以测试IS2B的工作假设 技术可以可靠地（i）样品散装和孔隙水污染物，以在子-NG/L范围内进行分析，（ii） 告知沉积物污染物的生物利用度和生物活性，（iii）告知人类暴露和 相关的鱼类消耗的健康风险，（iv）跟踪沉积物修复活动的进度。 实验室和现场研究将与来自阿波普湖（Lake Apopka）污染的沉积物进行，其中之一 佛罗里达州的各种超级基金网站。为了说明IS2B适用性的广度，该项目集中在 两种传统和三种新兴的沉积物污染物（P，P'-DDE，Dieldrin vessus vipronil，Triclosan， Triclocarban）。因为通过从建模中预测的生物积累之间可能存在差异 采样器数据和实际的生物积累，生物利用度和污染物的生物积累量将在 通过确定身体的新鲜峰值和长期污染的沉积物的实验实验 两种测试生物的负担：lumbriculus variegatus和pimephales promelas。生物学反应 还将使用DNA微阵列分析在鱼类中测量污染物暴露，以评估可能 不仅可以仅基于父母化合物的身体负担进行预测。数学关系 大量水，孔隙水，蠕虫和鱼类中的污染物浓度将从理论和实验室数据中提出 并应用于IS2B衍生的数据，以预测风险并跟踪两种补救方法的有效性， 颗粒状活化碳（GAC）修正案和受污染沉积物的深耕作。