Console for an 800/89 DNP Spectrometer

800/89 DNP 光谱仪控制台

• 批准号: 8448486
• 负责人: ROBERT Guy GRIFFIN
• 金额: $ 53.9万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-25 至 2014-06-24
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8448486
• 关键词: Amyloid Fibrils; Amyloid Proteins; Amyloidosis; Area; Bacteriorhodopsins; Biological; Boston; Communities; Dialysis procedure; Funding; Funding Applicant; Future; Gases; Grant; Investigation; Lipid Bilayers; M2 protein; Magic; Membrane; Membrane Proteins; North America; Nuclear; Phosphatidylinositols; Phosphotransferases; Proteins; Research; Research Project Grants; SH3 Domains; Sampling; Scientist; System; Techniques; Temperature; Testing; Vacuum; Vesicle; Voltage-Dependent Anion Channel; beta-2 Microglobulin; cold temperature; influenzavirus; innovative technologies; instrument; instrumentation; nanodisk; prototype; research study; solid state nuclear magnetic resonance; structural biology

DESCRIPTION (provided by applicant): Funding is requested for the purchase of three essential items that will be used with a 800/89 magnet: (1) a spectrometer console, (2) room temperature shims and (3) a magic angle spinning (MAS) probe with a 1.3 mm stator rotor system. The magnet was delivered in February and is presently at field. In the immediate future the spectrometer will be operated as a high field MAS NMR with low temperature capabilities. However, in the long term it will be converted to an 800 MHz/527 GHz DNP instrument. With funds from another grant we purchased a probe capable of performing experiments in the 90-300 K range and will be used to examine a variety of temperature dependent phenomena in cryoprotected membrane protein and amyloid protein samples. The probe has a vacuum jacketed dewar, sample eject capabilities, and includes a waveguide for dynamic nuclear polarization (DNP) experiments at 527 GHz. Research projects include the following investigations of membrane and amyloid proteins: (1) bacteriorhodopsin (bR) photocycle intermediates in native membranes and (2) nanodiscs, (3) the voltage dependent anion channel (VDAC) in lipid bilayers, (4) the M2 protein from influenza virus, (5) gas vesicles which form a proteinaceous membrane, and amyloid fibrils formed from (6) phosphatidyl-inositol-3-kinase SH3 domain (PI3- SH3), (7) beta-2-microglobulin associated with dialysis related amyloidosis, and (8) the CsgA protein, from Curli. In addition, this will be a prototype instrument performing DNP experiments at 800/527 and will (9) be used to test new biradical polarizing agents, (10) develop high field 17O MAS experiments and (11) new techniques for dipole recoupling at high ?r/2?. The 800/89 will be part of a cluster of instrumentation that serves a community of scientist performing high field biological NMR in the Boston area and North America. It will alleviate a pressing need for additional capabilities for low temperature and high field DNP/ MAS experiments. Further, the instrument will have a significant impact on research since it will employ a number of innovative technologies that are not available elsewhere. For example, the combination of the LT-MAS probe with the 800/89 will be one of the few widebore, low temperature solid state NMR instruments available worldwide and the addition of a 527 GHz gyrotron will make the system a unique DNP spectrometer in North America. It will therefore fill an important void in structural biology.

描述（由申请人提供）：要求购买三个必需物品，这些物品将与800/89磁铁一起使用：（1）光谱仪控制台，（2）室温垫片和（3）带有1.3 mm定子转子系统的神奇角度旋转（MAS）探针（MAS）探针。磁铁在2月交付，目前在现场。在不久的将来，光谱仪将作为具有低温功能的高场MAS NMR运行。但是，从长远来看，它将转换为800 MHz/527 GHz DNP仪器。借助另一份赠款的资金，我们购买了一个能够在90-300 K范围内执行实验的探针，并将用于检查冷冻保护膜蛋白和淀粉样蛋白样品中各种温度依赖性现象。该探针具有真空外套的露水，样品喷射能力，并包括用于动态核极化的波导（DNP）实验，在527 GHz处进行。 Research projects include the following investigations of membrane and amyloid proteins: (1) bacteriorhodopsin (bR) photocycle intermediates in native membranes and (2) nanodiscs, (3) the voltage dependent anion channel (VDAC) in lipid bilayers, (4) the M2 protein from influenza virus, (5) gas vesicles which form a proteinaceous membrane, and由（6）磷脂酰 - 肌醇-3-激酶SH3结构域（PI3-SH3），（7）与透析相关淀粉样变性相关的β-2-微球蛋白以及（8）CSGA蛋白，来自curli的蛋白。此外，这将是一种在800/527进行DNP实验的原型仪器，将（9）用于测试新的Biradical偏振剂，（10）开发高场17O MAS实验和（11）在高度？r/2上进行偶极子重耦合的新技术。 800/89将是为科学家社区服务的一组仪器的一部分 在波士顿地区和北美进行高田间生物NMR。它将减轻对低温和高场DNP/ MAS实验的额外功能的紧迫需求。此外，该工具将对研究产生重大影响，因为它将采用许多其他地方无法使用的创新技术。例如，LT-MAS探针与800/89的组合将是全球可用的少数宽温度，低温固态NMR仪器之一，并且添加527 GHz Gyrotron将使该系统成为北美独特的DNP光谱仪。因此，它将填充结构生物学的重要空隙。