CENTER FOR THE ANALYSIS OF CELLULAR MECHANISMS AND SYSTEMS BIOLOGY

细胞机制和系统生物学分析中心

• 批准号: 8359565
• 负责人: EDWARD A DRATZ
• 金额: $ 51.9万
• 依托单位: MONTANA STATE UNIVERSITY - BOZEMAN
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8359565
• 关键词: Advisory Committees; Area; Biological; Cells; Chemicals; Committee Members; Computer software; DNA Microarray Chip; Data; Detection; Development; Digestion; Disease; Faculty; Funding; Genomics; Goals; Grant; Mass Spectrum Analysis; Mentors; Metabolic; Methods; Microfluidics; Modeling; Molecular; Montana; National Center for Research Resources; Organic Synthesis; Post-Translational Protein Processing; Principal Investigator; Proteins; Proteomics; Reagent; Recovery; Research; Research Infrastructure; Research Personnel; Resources; Seeds; Source; System; Systems Biology; Training; United States National Institutes of Health; Work; cost; enzyme activity; improved; liquid chromatography mass spectrometry; mass spectrometer; member; metabolomics; multidisciplinary; nano; pointed protein; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The goal of this project is to develop a multidisciplinary Center for the Analysis of Cellular Mechanisms and Systems Biology, to provide new infrastructure to advance understanding of cellular mechanisms, to better understand how to overcome disease, and to build a critical mass of faculty in this area. The Center is unique in Montana and the five surrounding states, and will include a combination of capabilities for analysis of cellular mechanisms. This project will create a powerful Mass Spectrometry and Proteomics Core infrastructure, oriented toward training researchers. Most cellular mechanisms appear to be controlled by protein post-translational modifications and the MS and Proteomics Core will add new mass spectrometers for improved characterization of modifications of proteins, high sensitivity microfluidic nano LC/MS/MS, a Hyperspectral imager for enhanced multicolor proteomic multiplexing to pin-point proteins that change with biological stimulation, and a protein electro-elution and microfluidic digestion system for high efficiency recovery of pin-pointed proteins. Sensitive differential protein detection capabilities developed at MSU and high sensitivity microfluidic nano LC/MS/MS will be included in the Core. New software will integrate data from an existing DNA microarray Facility. A Proteomics Reagent Synthesis Core will prepare new fluorescent reagents for differential analysis of changes in enzyme activity and post-translational modifications that result from biological stimulation. Critical mass will be built by supporting the development of three junior faculty and one senior faculty member, who is charting a new course in his research. Outstanding local and national mentors and External Advisory Committee members will guide the development of these faculty and the Center. Over the period of the project we plan to hire four new faculty in areas needed to build critical mass: a Chemical Biologist/Organic Synthesis expert, a Biological Mass Spectroscopist using advanced MS tools, a Systems Biologist working on metabolic modeling and metabolomics, and a Cell Biologist using global molecular tools. Outstanding MSU Institutional Support, the significance and timeliness of the post-genomic analysis of cellular mechanisms, and the orientation toward training in advanced methods will seed new projects-and will result in significant scientific contributions and a sustainable Center.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 该项目的目标是建立一个多学科的细胞机制和系统生物学分析中心，提供新的基础设施来促进对细胞机制的理解，更好地了解如何克服疾病，并在这方面建立足够的师资队伍。区域。该中心在蒙大拿州和周边五个州是独一无二的，并将包括细胞机制分析功能的组合。该项目将创建一个强大的质谱和蛋白质组学核心基础设施，面向培训研究人员。大多数细胞机制似乎是由蛋白质翻译后修饰控制的，MS 和蛋白质组学核心将添加新的质谱仪以改进蛋白质修饰的表征、高灵敏度微流体纳米 LC/MS/MS、用于增强多色蛋白质组学多重分析的高光谱成像仪精确定位随生物刺激而变化的蛋白质，以及蛋白质电洗脱和微流体消化系统，以高效回收精确定位的蛋白质。 密歇根州立大学开发的灵敏差异蛋白检测功能和高灵敏度微流控纳米 LC/MS/MS 将包含在核心中。新软件将整合现有 DNA 微阵列设施的数据。蛋白质组学试剂合成核心将制备新的荧光试剂，用于差异分析生物刺激引起的酶活性变化和翻译后修饰。 将通过支持三名初级教师和一名正在制定研究新课程的高级教师的发展来建立临界质量。优秀的本地和国家导师以及外部咨询委员会成员将指导这些教师和中心的发展。在该项目期间，我们计划在建立临界质量所需的领域聘用四名新教职人员：一名化学生物学家/有机合成专家、一名使用先进 MS 工具的生物质谱学家、一名致力于代谢建模和代谢组学的系统生物学家以及一名使用全球分子工具的细胞生物学家。密歇根州立大学出色的机构支持、细胞机制后基因组分析的重要性和及时性，以及先进方法培​​训的方向，将孕育新的项目，并将带来重大的科学贡献和可持续发展的中心。