CENTER FOR THE ANALYSIS OF CELLULAR MECHANISMS AND SYSTEMS BIOLOGY

细胞机制和系统生物学分析中心

• 批准号: 7721041
• 负责人: EDWARD A DRATZ
• 金额: $ 78.36万
• 依托单位: MONTANA STATE UNIVERSITY - BOZEMAN
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-26 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721041
• 关键词: Advisory Committees; Area; Biological; Cells; Chemicals; Computer Retrieval of Information on Scientific Projects Database; Computer software; DNA Microarray Chip; DNA Microarray format; Data; Detection; Development; Disease; Faculty; Funding; Genomics; Goals; Grant; Institution; Mass Spectrum Analysis; Mentors; Metabolic; Methods; Microfluidics; Modeling; Modification; Molecular; Montana; Organic Synthesis; Post-Translational Protein Processing; Proteins; Proteomics; Reagent; Recovery; Research; Research Infrastructure; Research Personnel; Resolution; Resources; Seeds; Source; System; Systems Biology; Training; United States National Institutes of Health; Work; enzyme activity; improved; member; metabolomics; multidisciplinary; nano; pointed protein; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Description (provided by applicant): The goal of this application is to develop a multidisciplinary Center for the Analysis of Cellular Mechanisms and Systems Biology, to provide new infrastructure to advance understanding of cellular mechanisms, to better understand how to overcome disease, and to build a critical mass of faculty in this area. The Center will be unique in Montana and the five surrounding states, and will include a combination of capabilities for analysis of cellular mechanisms. This project will create a powerful Mass Spectrometry and Proteomics Core infrastructure, oriented toward training researchers. Most cellular mechanisms appear to be controlled by protein post-translational modifications and the MS and Proteomics Core will add: a high resolution Qtof MS for improved "bottom up" characterization of protein post-translational modifications, an FTMS for "top-down" characterization of modifications on intact proteins, a Hyperspectral imager for enhanced multicolor proteomic multiplexing to pin-point proteins that change with biological stimulation, and a protein electro-elution system for high efficiency recovery of pin-pointed proteins. Sensitive differential protein detection capabilities developed at MSU and high sensitivity microfluidic nano LC/MS/MS will be included in the Core. New software will integrate data from an existing DNA microarray Facility. A Proteomics Reagent Synthesis Core will prepare new fluorescent reagents for differential analysis of changes in enzyme activity and post-translational modifications that result from biological stimulation. We will build critical mass by first supporting the development of three young faculty and one senior faculty member, who is charting a new course in his research. Outstanding local and national mentors and External Advisory Committee members will guide the development of these faculty and the Center. Second, we will hire four new faculty in areas needed to build critical mass: a Chemical Biologist/Organic Synthesis expert, a Biological Mass Spectroscopist using advanced FTMS tools, a Systems Biologist working on metabolic modeling and metabolomics, and a Cell Biologist using global molecular tools. Outstanding MSU Institutional Support ($1,400,000), the significance and timeliness of the post-genomic analysis of cellular mechanisms, and the orientation toward training in advanced methods will seed new projects-and will result in significant scientific contributions and a sustainable Center.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 描述（由申请人提供）：本申请的目标是建立一个多学科的细胞机制和系统生物学分析中心，提供新的基础设施来促进对细胞机制的理解，更好地理解如何克服疾病，并建立该领域拥有足够数量的教师。该中心在蒙大拿州和周边五个州将是独一无二的，并将包括细胞机制分析功能的组合。该项目将创建一个强大的质谱和蛋白质组学核心基础设施，面向培训研究人员。大多数细胞机制似乎是由蛋白质翻译后修饰控制的，MS 和蛋白质组学核心将添加：高分辨率 Qtof MS，用于改进蛋白质翻译后修饰的“自下而上”表征，FTMS 用于“自上而下”表征对完整蛋白质进行修饰，用于增强多色蛋白质组多重分析以精确定位随生物刺激而变化的蛋白质的高光谱成像仪，以及用于高效回收精确蛋白质的蛋白质电洗脱系统。灵敏差速器 密歇根州立大学开发的蛋白质检测功能和高灵敏度微流控纳米 LC/MS/MS 将包含在核心中。新软件将整合现有 DNA 微阵列设施的数据。蛋白质组学试剂合成核心将制备新的荧光试剂，用于差异分析 由生物刺激引起的酶活性和翻译后修饰的变化。 我们将首先支持三名年轻教师和一名正在制定研究新课程的高级教师的发展，以建立起足够的规模。优秀的本地和国家导师以及外部咨询委员会成员将指导这些教师和中心的发展。其次，我们将在建立临界质量所需的领域聘请四名新教师：一名化学生物学家/有机合成专家、一名使用先进 FTMS 工具的生物质谱学家、一名致力于代谢建模和代谢组学的系统生物学家以及一名使用全球分子生物学的细胞生物学家。工具。杰出的密歇根州立大学机构支持（1,400,000 美元）、细胞机制后基因组分析的重要性和及时性，以及先进方法培​​训的方向将孕育新的项目，并将带来重大的科学贡献和可持续发展的中心。