MicroRNA determinants of oral cancer detection

口腔癌检测的 MicroRNA 决定因素

基本信息

批准号：
8510964
负责人：
XIAOFENG ZHOU
金额：
$ 7.98万
依托单位：
UNIVERSITY OF ILLINOIS AT CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-04-01 至 2015-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8510964
关键词：
Biochemical Bioinformatics Biological Assay Biological Markers Biomedical Engineering Biometry Cancer Detection Cancer Patient Carcinoma Cessation of life Classification Clinical Code Collecting Cell Cytology Dentistry Detection Development Diagnostic Diagnostic tests Disease Early Diagnosis Engineering Epithelium Event Foundations Functional RNA Genes Genomics Hour Knowledge Lead Malignant Neoplasms Measures Methods MicroRNAs Modeling Molecular Molecular Genetics Nature Nucleic Acids Operative Surgical Procedures Oral Oral Medicine Oral Surgical Procedures Oral cavity Outcome Study Patients Pattern Persons Procedures Process Proteins RNA marker Radiation therapy Research Personnel Resources Role Sampling Science Screening for Oral Cancer Smoker Staging Technology Therapeutic Intervention Time Translational Research Universities Validation base cancer initiation cancer therapy cancer type candidate identification chemotherapy college high risk improved killings malignant mouth neoplasm minimally invasive mouth squamous cell carcinoma novel oral diagnostics oral lesion oral surgery specialty outcome forecast programs public health relevance tool tumor tumor progression tumorigenesis

项目摘要

DESCRIPTION (provided by applicant): Oral cancer is one of the most common cancers. In the US there are over 39,000 new cases of oral cancer each year. It causes approximately 8,000 deaths per year, killing roughly 1 person per hour. One of the major clinical problems of oral cancer is the late detection of the disease. Improvement in patient survival requires a better understanding of oral cancer initiation and progression, so that aggressive tumors can be detected early in the disease process and targeted therapeutic interventions can be deployed. While many studies have been devoted to investigate molecular events in tumorigenesis of oral cancer, most efforts are focused on protein coding genes. The knowledge on the role of non-coding genes (e.g., microRNA) in oral cancer is relatively limited. Our recent studies have demonstrated critical contributions of microRNA deregulation in oral cancer. However, the comprehensive microRNA alteration pattern associated with early stage oral cancer is still lacking, which hindered the potential utilization of microRNA as diagnostic biomarkers. Brush oral cytology is a minimally-invasive method that can be used to collect cells from suspect oral lesions for histological and biochemical analyze. Our recent results demonstrated that we can consistently measure RNA markers in the brush oral cytology samples. In this study, we will identify unique microRNA signatures that associated with early stage oral cancer from the brush oral cytology samples (Specific Aim 1). We will then test the diagnostic values of these microRNA markers in an independent sample set (Specific Aim 2). Statistical classification models will be developed to assess the diagnostic values of these microRNAs. This proposal combines minimally-invasive methods with the emerging genomic technologies to harness microRNA determinants in oral cancer. The outcomes from this study will provide strong rationale and scientific foundation for more extensive patient- based studies to identify and validate microRNA biomarkers for early detection of oral cancer.

描述（由申请人提供）：口腔癌是最常见的癌症之一。在美国，每年有超过 39,000 例新发口腔癌病例。它每年导致大约 8,000 人死亡，每小时大约杀死 1 人。口腔癌的主要临床问题之一是疾病的晚期发现。提高患者生存率需要更好的了解口腔癌的发生和进展，以便在疾病过程的早期发现侵袭性肿瘤，并采取有针对性的治疗干预措施。虽然许多研究致力于调查口腔癌肿瘤发生中的分子事件，但大多数努力都集中在蛋白质编码基因上。关于非编码基因（例如 microRNA）在口腔癌中的作用的知识相对有限。我们最近的研究证明了 microRNA 失调对口腔癌的关键作用。然而，与早期口腔癌相关的全面的microRNA改变模式仍然缺乏，这阻碍了microRNA作为诊断生物标志物的潜在利用。刷口腔细胞学是一种微创方法，可用于从可疑口腔病变中收集细胞进行组织学和生化分析。我们最近的结果表明，我们可以一致地测量刷口腔细胞学样本中的 RNA 标记。在这项研究中，我们将从刷口腔细胞学样本中识别与早期口腔癌相关的独特 microRNA 特征（具体目标 1）。然后，我们将在独立样本集中测试这些 microRNA 标记的诊断价值（具体目标 2）。将开发统计分类模型来评估这些 microRNA 的诊断价值。该提案将微创方法与新兴基因组技术相结合，以利用口腔癌中的 microRNA 决定因素。这项研究的结果将为更广泛的基于患者的研究提供强有力的理论依据和科学基础，以识别和验证用于早期检测口腔癌的 microRNA 生物标志物。