Mouse Genetics Core

小鼠遗传学核心

基本信息

批准号：
8110617
负责人：
ALCINO J. SILVA
金额：
$ 23.35万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-04-01 至 2012-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8110617
关键词：
Back Brain Equilibrium Gene Mutation Genetic Genetic Screening Genotype Laboratories Mus Mutant Strains Mice Mutation Partner in relationship Phenotype Role Speed Techniques Testing congenic

项目摘要

All three projects in the Center require mutant mice derived at UCLA. The Mouse Genetics Core will reside in a SPF facility at UCLA and will facilitate the back-cross, mating and dissemination of the mutant mice required by all three Projects in the center. The facility will maintain the lines, genotype them and send them when needed to the participating laboratories. An important role for the facility will be to transfer mutations into stable genetic backgrounds that could then be retested and sent to the laboratories in the Center. Some of the mice that will be tested in the genetic screen proposed in Project 1 are not in stable, balanced genetic backgrounds. The Mouse Genetics Core will use speed congenics and other techniques to transfer selected mutations into stable and defined genetic backgrounds. The facility will also be responsible for the distribution of mice with conditional mutations of genes identified in the plasticity screens proposed. The integration of studies in the Center is completely dependent on the uniformity of the genetic background of the mice tested. Since phenotypes, including brain phenotypes, are sensitive to genetic background, it would be impossible to integrate findings from different laboratories in the Center, if each laboratory used mice with varying genetic background. Thus, providing mice of uniform genetic background is a crucial and key function of the Mouse Genetics Core.

该中心的所有三个项目都需要来自加州大学洛杉矶分校的突变小鼠。小鼠遗传学核心将驻留在加州大学洛杉矶分校的 SPF 设施，将促进突变小鼠的回交、交配和传播中心所有三个项目都需要。该设施将维护这些细胞系，对其进行基因分型并将其发送需要时向参与实验室提供。该设施的一个重要作用是转移突变进入稳定的遗传背景，然后可以重新测试并发送到中心的实验室。一些将在项目 1 中提出的基因筛选中进行测试的小鼠的遗传状况不稳定、不平衡背景。小鼠遗传学核心将使用速度同类基因和其他技术来转移选定的突变为稳定且明确的遗传背景。该设施还将负责在可塑性筛选中发现的具有条件性基因突变的小鼠的分布。这该中心的研究整合完全取决于遗传背景的一致性老鼠进行了测试。由于表型，包括大脑表型，对遗传背景很敏感，因此如果每个实验室都使用小鼠，则不可能整合该中心不同实验室的研究结果不同的遗传背景。因此，提供具有统一遗传背景的小鼠是至关重要的小鼠遗传学核心的功能。