Animal Core

动物核心

基本信息

批准号：
8588580
负责人：
JOHN F DE GROOT
金额：
$ 24.27万
依托单位：
UNIVERSITY OF TX MD ANDERSON CAN CTR
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-09-01 至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8588580
关键词：
Animal Experiments Animals Brain Brain Neoplasms Breeding Cell Line Cells Data Evaluation Experimental Designs Funding Genes Genetically Engineered Mouse Glial Fibrillary Acidic Protein Glioma Harvest Health Human Human Resources Immunocompetent Immunodeficient Mouse Implant Injection of therapeutic agent Intraperitoneal Injections Laboratory Technicians Leadership Malignant neoplasm of brain Mediating Mesenchymal Modeling Monitor Mus Oral Patients Principal Investigator Process Protocols documentation Reporting Research Infrastructure Research Personnel Resistance Services Solid Neoplasm Stem cells System Techniques Tetanus Helper Peptide Therapeutic TimeLine Tissues U251 Virus Work Writing Xenograft Model Xenograft procedure cell type experience glioma cell line human disease implantation interest meetings member mouse model nestin protein relating to nervous system research study tumor tumorigenesis

项目摘要

In next 5-year funding period, investigators in Brain Tumor SPORE will continue to utilize the expert services provided by the Animal Core. The majority of projects in the SPORE rely on intracranial (orthotopic) implantation of established, adherent glioma cell lines, which are molecularly well characterized. Improvements in modeling the human disease will be achieved through the use of orthotopic xenografts of human glioma stem cells (GSCs), which are an important component of solid tumors, better replicate the human disease, and may mediate treatment resistance. These patient-derived GSC cell lines (N>50 to date) have been molecularly characterized into proneural, mesenchymal, classical and neural subtypes. In addition, genetically engineered mouse models of glioma (GEMMs) such as the RCAS/Ntv-a system are utilized by SPORE investigators. Specific genes of interest important for tumorigenesis can be evaluated in this model in a cell-type specific manner. Other GEMMs available within the Animal Core include: hGFAP-Cre*;p5S '¿'^¿'';Pten '¿^^ and Nestin-CreERT2 clnk4a/Arf UL cPTEN UL GFAP-tta tet- EGFRviil. Importantly, tumors generated in immunocompetent mice allow for better evaluation of interactions between tumor and the native microenvironment. Finally, we will continue to maintain and provide assistance with the establishment of traditional xenograft models from standard glioma cell lines (e.g. U87, U251, LN229, etc.). The Specific Aims of the Animal Core are: Aim 1: Provide support for animal experiments using patient derived glioma stem cell lines (GSCs) as orthotopic xenografts in immunodeficient mice. Aim 2: Provide support for animal experiments using Genetically Engineered Mouse Models (GEMMs). Aim 3: Maintain working stocks of cell lines. Aim 4: Provide support for xenografts of standard glioma cell lines. RELEVANCE: DO NOT EXCEED THE SPACE PROVIDED. Three of the four Brain Turiior SPORE projects are expected to use significant numbers of mice for their experiments. The Brain Tumor Animal Core will provide expert oversight of the animal experiments. Experienced personnel will execute the experimental plans.

在下一个5年的资金期间，脑肿瘤孢子的调查人员将继续使用专家动物核心提供的服务。（原位）植入已建立的粘附神经胶质瘤细胞系，它们的分子很好特征是建模人类疾病的改进人神经胶质瘤干细胞（GSC）的异种，这是实体瘤的重要组成部分，更好复制人类疾病，可能介导这些患者衍生的GSC细胞系（迄今为止n> 50）已被分子表征为胸膜，间充质，经典和神经亚型。系统被孢子研究者使用。在此模型中以细胞类型的特定方式进行评估。包括：hgfap-cre*; '^¿ '; ^^和NESTIN-CREERT2 CLNK4A/ARF UL CPTEN UL GFAP-TTA TET- egfrviil。肿瘤与天然微环境之间的相互作用。在建立来自标准神经胶质瘤细胞的传统Xenograph模型方面提供帮助（例如U87，U251，LN229等）。目标1：使用患者衍生的神经胶质瘤干细胞系（GSC）为动物实验提供支持免疫缺陷小鼠中的原位定量异构移植物。 AIM 2：使用基因工程的小鼠Mousels（GEMM）为动物实验提供支持。 AIM 3：维护细胞系的工作库存。 AIM 4：为标准神经胶质瘤细胞系的Xen仪提供支持。相关性：不要删除提供的空间。预计四元小孢子项目中有三个将使用大量的小鼠实验。经过经验的人员将执行实验计划。