PRIMATE PLURIPOTENT CELLS FOR AUTOLOGOUS TRANSPLANTATION

用于自体移植的灵长类多能细胞

• 批准号: 8173333
• 负责人: SHOUKHRAT M MITALIPOV
• 金额: $ 7.61万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8173333
• 关键词: Adult; Autologous Transplantation; Cells; Chimera organism; Computer Retrieval of Information on Scientific Projects Database; Embryo; Fibroblasts; Funding; Grant; Institution; Macaca mulatta; Monkeys; Primates; Research; Research Personnel; Resources; Skin; Somatic Cell; Source; Testing; United States National Institutes of Health; Validation; embryonic stem cell; induced pluripotent stem cell; pluripotency; somatic cell nuclear transfer

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The aim of this proposal is to investigate the potential of rhesus monkey pluripotent cells experimentally induced by reprogramming of adult somatic cells using two alternative approaches: somatic cell nuclear transfer (SCNT) and direct reprogramming. In this application, we will generate genetically identical SCNT-ES and iPS cells from adult monkey skin fibroblasts and compare them to each other and to ES cells produced from fertilized embryos using an array of extensive tests including the ability to generate chimeras, the ultimate validation of their pluripotency.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 该提案的目的是研究使用两种替代方法：体细胞核移植（SCNT）和直接重编程，通过对成年体细胞进行重编程来实验诱导恒河猴多能细胞的潜力。在此应用中，我们将从成年猴皮肤成纤维细胞中生成遗传相同的 SCNT-ES 和 iPS 细胞，并使用一系列广泛的测试（包括生成嵌合体的能力、最终验证）将它们相互比较以及与受精胚胎产生的 ES 细胞进行比较。他们的多能性。