DERIVING OOCYTES FROM EMBRYONIC STEM CELLS (ESCS)

从胚胎干细胞 (ESCS) 中获取卵母细胞

• 批准号: 8357829
• 负责人: SHOUKHRAT M MITALIPOV
• 金额: $ 4.36万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357829
• 关键词: Assisted Reproductive Technology; Cell Line; Cell Lineage; Embryo; Female; Funding; Germ Cells; Goals; Grant; Knock-in Mouse; Monkeys; National Center for Research Resources; Oocytes; Pluripotent Stem Cells; Primates; Principal Investigator; Research; Research Infrastructure; Resources; Source; United States National Institutes of Health; cost; embryonic stem cell; induced pluripotent stem cell; promoter; somatic cell nuclear transfer

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The goal of this proposal is to evaluate the potential of monkey pluripotent stem cells to contribute to the female germ cell lineage, and to explore the feasibility of deriving functional oocytes suitable for use in assisted reproductive technologies. Our main hypothesis is that primate pluripotent stem cells derived from fertilized embryos, experimentally reprogrammed by somatic cell nuclear transfer or direct reprogramming (iPS) approaches are capable of forming female germ cells and gametes upon spontaneous or directed differentiation. We produced GFP knock-in monkey ESC line under the control of germ cell specific promoters: OCT4 and VASA. These cell lines should aid in identification of germ cells during differentiation of ESCs.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 该提案的目标是评估猴多能干细胞对雌性生殖细胞谱系做出贡献的潜力，并探索衍生适用于辅助生殖技术的功能性卵母细胞的可行性。 我们的主要假设是，源自受精胚胎的灵长类多能干细胞，通过体细胞核移植或直接重编程（iPS）方法进行实验重编程，能够在自发或定向分化时形成雌性生殖细胞和配子。我们在生殖细胞特异性启动子 OCT4 和 VASA 的控制下产生了 GFP 敲入猴 ESC 系。这些细胞系应该有助于 ESC 分化过程中生殖细胞的鉴定。