PROGESTERONE RECEPTORS & ACTION IN PRIMATE OVARY

黄体酮受体

• 批准号: 8173179
• 负责人: RICHARD L STOUFFER
• 金额: $ 7.61万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8173179
• 关键词: Binding Proteins; Cell physiology; Computer Retrieval of Information on Scientific Projects Database; Corticotropin-Releasing Hormone; Development; Enzymes; Fertility; Funding; Gene Proteins; Genes; Graafian Follicles; Grant; Hormonal; Institution; Longevity; Luteinizing Hormone; Luteolysis; Menstrual cycle; Molecular; Monkeys; Ovarian; Ovary; Ovulation; Peptide Hydrolases; Periodicity; Pituitary Hormones; Primates; Process; Progesterone; Progesterone Receptors; Proteins; Research; Research Personnel; Resources; Source; Steroids; Structure; System; Testing; Tissues; United States National Institutes of Health; Woman; corpus luteum; genome-wide; protein expression; receptor binding; receptor expression; research study; steroid hormone; urocortin

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The objective of this research is to understand the mechanisms whereby hormonal and local factors control the structure-function of the ovulatory follicle and corpus luteum in the ovary, and hence fertility, during the menstrual cycle in primates. Ongoing research is testing the hypothesis that the vital actions of the pituitary hormone, luteinizing hormone (LH) in promoting ovulation of the mature follicle and its subsequent conversion into the corpus luteum, and in controlling the functional lifespan of the corpus luteum, is either direct or indirect via local steroid action (e.g., via stimulation of progesterone [P] synthesis and P receptor expression). Because of similarities to ovarian function in women, monkeys are used in experiments where either LH and steroid hormones are depleted or steroids alone are depleted and replaced. The ovulatory follicle or corpus luteum is then removed to analyze molecular and cellular processes that are LH-dependent and either steroid/P-independent or -dependent. Gene and protein expression are analyzed for various processes, including: (1) protease enzymes that may be critical for tissue remodeling during ovulation, luteal development and luteal regression, and (2) local regulatory systems, e.g., the corticotropin-releasing hormone (CRH)/urocortins (UCN)-receptor-binding protein system. Current genome-wide analyses are identifying processes that are regulated by LH and/or steroid hormones in the follicle of corpus luteum. The activity of these processes is being manipulated, via gene knockdown or protein antagonist approaches, to elucidate their biologic relevance to primate ovarian function and cyclicity.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 本研究的目的是了解激素和局部因素在灵长类动物月经周期中控制卵巢排卵卵泡和黄体的结构功能，从而控制生育力的机制。正在进行的研究正在验证以下假设：垂体激素、黄体生成素 (LH) 在促进成熟卵泡排卵及其随后转化为黄体以及控制黄体功能寿命方面的重要作用是直接的或通过局部类固醇作用间接（例如，通过刺激孕酮 [P] 合成和 P 受体表达）。由于与女性卵巢功能相似，猴子被用于实验，其中 LH 和类固醇激素被耗尽，或者单独类固醇被耗尽并被替换。然后取出排卵卵泡或黄体，以分析 LH 依赖性以及类固醇/P 独立或依赖性的分子和细胞过程。分析各种过程的基因和蛋白质表达，包括：（1）可能对排卵、黄体发育和黄体退化期间的组织重塑至关重要的蛋白酶，以及（2）局部调节系统，例如促肾上腺皮质激素释放激素（CRH） )/尿皮质素 (UCN)-受体结合蛋白系统。目前的全基因组分析正在识别黄体卵泡中受 LH 和/或类固醇激素调节的过程。通过基因敲低或蛋白质拮抗剂方法来操纵这些过程的活性，以阐明它们与灵长类动物卵巢功能和周期性的生物学相关性。